Effects of Different Concentrations of α-naphthaleneacetic Acid and 6-benzylaminopurine on Shoot Regeneration of Vinca minor L.

Raouf Fard, F.; Moieni, A.; Omidbaigi, R.

Effects of Different Concentrations of α-naphthaleneacetic Acid and 6-benzylaminopurine on Shoot Regeneration of Vinca minor L.

Authors

F. Raouf Fard ¹

A. Moieni ¹

R. Omidbaigi ²

¹ Department of Horticulture, College of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

² Department of Plant Breeding, College of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

Abstract

Periwinkle (Vinca minor L.) is an evergreen perennial herbaceous plant that belongs to the family Apocynaceae. The aerial part of this plant contains certain alkaloids such as vincamine, isovincamine and vincine. The most important of these is vincamine that re-duces blood pressure and promotes memory and the ability for mental concentration. Mi-cropropagation could be a good alternative for the mass propagation of Vinca minor. For direct shoot regeneration, single node explants of field-grown Vinca minor cv. Budakalasz were aseptically cultured on a medium consisting of WPM salts, MS vitamins, 3% su-crose, 0.8% agar and 25 different combinations of BAP and NAA. After one month, ex-plants were subcultured to the same medium. The number of shoots (taller than 3 mm), average shoot length, height of the longest shoot, callus amount, number of roots, and av-erage root length per explant were measured two months after culture. The maximum shoot regeneration (5.6 shoots per explant) was obtained using 7.21 mg l-1 BAP and 0.0186 mg l-1 NAA.

Keywords

Shoot regeneration

Callogenesis

In vitro

Vinca minor L