Optimization of Steam Treatment Conditions for Improving the Nutritive Value of Date Leaves

Authors
M. Zahedifar 1
N. Karimi 2
H. Fazaeli 1
S. A. Mirhadi 1
1 Animal Science Research Institute of Iran, Department of Animal Nutrition, Karaj, Islamic Republic of Iran.
2 Islamic Azad University, Pishva Branch
Abstract
The aim of this study was to investigate the effect of steam treatment on nutritive value of date (Phoenix dactylifera) leaves. Date leaves were chopped and mixed with water or sulfuric acid solution to contain 50% moisture with or without 1% sulfuric acid. Steam treatment of the date leaves was carried out using three levels of steam pressure (14, 17 and 20 bar), three reaction times (120, 180, and 240 seconds) and two levels of acid (0 and 1 percent). The treated samples were analyzed for chemical composition including: cell wall components, ash, total extractable phenolics, water soluble sugars, and reducing sugars. Dry matter loss (DML), enzymic hydrolysis, and in vitro gas production of the samples were also measured. Results showed that steam treatment significantly affected (P< 0.05) cell wall components. An increasing trend was observed in DML by increasing harshness of treatment conditions. The lowest DML (12.7 g kg-1) was observed in the auto-hydrolyzed (steam treatment without addition of exogenous acid) sample treated at 14 bar pressure and 120 seconds reaction time and the highest DML (78.8 g kg-1) was observed in the acid-hydrolyzed (addition of 10 g kg-1acid prior to treatment) samples treated at 20 bar pressure and 180 and 240 seconds reaction times. Steam treatment significantly (P< 0.05) decreased neutral detergent fiber (NDF) content but increased acid detergent lignin (ADL). Maximum changes in hemicellulose and water soluble sugars were observed in acid-hydrolyzed samples, in which hemicellulose decreased from 264.6 g kg-1 in control to 72.2 g kg-1 in the sample treated at 20 bar and 240 seconds and water soluble sugars increased from 14.0 g kg-1 in the control to 101.8 g kg-1 in the sample treated at 17 bar and 240 seconds. Enzymic hydrolysis of date leaves was improved after steam treatment and higher improvement was observed in acid-hydrolyzed samples. Gas production was significantly increased (P< 0.05) in all incubation times after steam treatment. The maximum increase in metabolizable energy (ME) estimated by gas production was from acid-hydrolyzed sample treated at 20 bar and 240 seconds. In auto-hydrolyzed samples, the biggest increase in ME was observed in the sample treated at 20 bar and 180 seconds. The results suggest that steam treatment could be used for upgrading the nutritive value of date leaves in the regions where date is grown and animals are encountered with severe feed shortage.

Keywords

1. AOAC. 2000. Official Methods of Analysis. 17th Edition, Association of Official Analytical Chemists, Gaithersburg, MD.
2. Arhab, R., Macheboeuf, D., Doreau, M. and Bousseboua, H. 2006. Nutritive Value of Date Palm Leaves and Aristida pungens Estimated by Chemical, In vitro and In situ Methods. Tropical Subtropical Agroecosystems, 6: 167-175.
3. Bacon, J. S. D. and Gordon, A. H. 1980. The Effect of Various Deacetylation Procedures on the Nylon Bag Digestibility of Barley Straw and of Grass Cell Walls Recovered from Sheep Faeces. J. Agric. Sci. Camb., 94: 361 367.
4. Bacon, J. S. D., Gordon, A. H. and Morris, E. J. 1975. Acetyl Group in Cell Wall Preparations from Higher Plants. Biochem., J. 149: 485 487.
5. Baugh, K. D. and McCarty, P. L. 1988. Thermochemical Pretreatment of Lignocellulose to Enhance Methane Fermentation. 1. Monosaccharide and Furfurals Hydrothermal Decomposition and Product Formation Rates. Biotechnol. Bioeng. J., 31: 50-61.
6. Belal, I., Al-Jasser, M., Mustafa, I. and Al-Dosari, M. 1999. Evaluation of Date-Feed Ingredients Mixes. Anim. Feed Sci. Tech., 81: 297-298.
7. Blummel, M. and Becker, K. 1997. The Degradability Characteristics of Fifty-four Roughages and Roughage Neutral-Detergent Fibers as Described by In vitro Gas Production and Their Relationship to Voluntary Feed Intake. British J. Nutrition., 77: 757-768.
8. Bukhaev, V. T., Al- Haydari, S. O., Abbas, M. F. and Maysera, M. S. 1985. Chemical and Biological Studies on Date Palm Parts and By- Products for Use as Feed-Stuffs for Ruminants. Zanco. 3(4): 7-16.
9. Carrasco, J. E., Saiz, M. C., Navarro, A., Soriano, P., Saef, F. and Martinez, M. 1994. Effect of Dilute Acid and Steam Explosion Pretreatments on the Cellulose Structure and Kinetics of Cellulosic Fraction Hydrolysis by Dilute Acids in Lignocellulosic Materials. Appl. Biochem. Biotechnol., 45/46: 23-34.
10. Castro, F. B. 1994. The Use of Steam Treatment to Upgrade Lignocellulosic Materials for Animal Feed. PhD. Thesis, University of Aberdeen, Scotland, UK.
11. Castro, F. B., Hotton, P. M. and E. R. Ørskov 1993a. The Potential of Dilute-acid Hydrolysis as a Treatment for Improving the Nutritional Quality of Industrial Lignocellulosic By-products. Anim. Feed Sci. Tech., 42: 39-54.
12. Castro, F. B., Hotton, P. M. and Ørskov E. R. 1993. Effect of Dilute-acid Hydrolysis Treatment on the Physico-chemical Features and Bio-utilization of Wheat Straw. Anim. Feed Sci. Tech., 42: 55-67.
13. Chaji, M. Mohammadabadi, T., Mamouei, M. and Tabatabaei, S. 2010a. The Effect of Processing with High Steam and Sodium Hydroxide on Nutritive Value of Sugarcane Pith by In vitro Gas Production. J. Anim. Veterinary Advances, 9(6): 1015-1018
14. Chaji, M., Mohammadabadi T. and Aghaei, A. 2011. Fermenting Cell Walls of Processed Sugarcane Pith by Ruminal Bacteria, Protozoa and Fungi. International J. Agriculture Biology, 13: 283–286
15. Chaji, M., Naserian A.,Valizadeh, R. Mohammadabadi, T. 2013. Physico-chemical Properties of Steam Treated Sugarcane Pith and Some Roughages and Their Role in Ruminants Nutrition. Iranian J. Anim. Sci. Research, 4(4): 298-309.
16. Chaji, M., Naserian, A., Valizadeh, R., Mohammadabadi, T. and Mirzadeh, K. 2010b. Potential Use of High Temperature and Low Temperature Steam Treatment, Sodium Hydroxide and an Enzyme Mixture for Improving the Nutritional Value of Sugarcane Pith. South African J. Anim. Sci., 40: 22–31
17. Chua, M. G. S. and Wayman, M. 1979. Characterization of Autohydrolysis Aspen (P. tremuloides) Lignins. Part 1. Composition and Molecular Weight Distribution of Extracted Autohydrolysis Lignin. Can. J. Chem., 57: 1141-1149.
18. Cunningham, R. L. and Carr, M. E. 1984. Pretreatments of Wheat Straw for Separation into Major Components. Biotechnol. Bioeng. Symp., 14: 95-103.
19. Dekker, R. F. H. and Wallis, A. F. A. 1983. Enzymic Saccharification of Sugar Cane Bagasse Pretreated by Autohydrolysis Steam Explosion. Biotechnol. Bioeng., 25: 3027 3048.
20. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. 1956. Colorimetric Method for Determination of Sugars and Related Substances. Anal. Chem., 28: 350-356.
21. Grohmann, K., Torget, R., and Himmeel, M. 1985. Optimization of Dilute Acid Pretreatment of Biomass. Biotechnol. Bioeng. Symp., 15: 59-80.
22. Hodge, J. E. 1953. Dehydrated Foods. Chemistry of Browning Reactions in Model Systems. Agric. Food. Chem., 1: 928-943.
23. Julkunen Tiitto, R. 1985. Phenolic Constituents in the Leaves of Northern Willows: Methods for the Analysis of Certain Phenolics. J. Sci. Food Agric., 33: 213 217.
24. Kardooni, A. 2012. Utilization of Uurea Treated Date Palm Leaves in Fattening of Buffalo Calves: Final Report. Khuzestan Research Center for Agricultural and Natural Resources, Iran.
25. Kawamoto, H., Mohamad, W. Z. and Oshio, O. 2001. Effect of Processing Methods on Digestibility and Voluntary Intake of Oil Palm Leaves in Cattle., Bulletin of the National Grassland Research Institute, 59: 10-17
26. Liu, J. X., Ørskov, E. R. and Chen, X. B. 1999. Optimization of Steam Treatment as a Method for Upgrading Rice Straw as Feeds. Anim. Feed. Sci. Technol., 76: 345-357.
27. Liu, J. X. and Ørskov E. R. 2000. Cellulase Treatment of Untreated and Steam pre-treated Rice Straw Effect on In vitro Fermentation Characteristic. Anim. Feed Sci. Techol., 88: 189–200
28. Makkar, H. P. S., Borrowy, N. K. and Becker, K. (1992) Quantification of Polyphenols in Animal Feedstuffs. Proceeding of the 15th International conference of group polyphenol, Lisboa, Portugal (13-17th July).
29. Medjekal, S., Arhab, R. and Bousseboua, H. 2011. Nutritive Value Assessment of Some Desert By-products by Gas Production and Rumen Fermentation. In vitro Livestock Research for Rural Development, 23(3).
30. Menke, K. H. and Steingass, H. 1987. Estimation of the Energetic Feed Value Obtained from Chemical Analysis and In vitro Gas Production Using Rumen Fluid. An. Res. Develop. Separate Print., 28: 7-55.
31. Mosier, N. S., Ladisch, C. M. and Ladisch, M. R. 2002. Characterization of Acid Catalytic Domains for Cellulose Hydrolysis and Glucose Degradation. Biotechnol. Bioeng., 20: 610-618.
32. Nelson, N. 1944. A Photometric Adaptation of the Somogyi Method for the Determination of Glucose. J. Biol. Chem., 153: 375- 380.
33. Oji, U. I. and Mowat, D. N. 1978. Nutritive Value of Steam Treated Corn Stover. Can. J. Anim. Sci., 58:177-181.
34. Oji, U. I. and Mowat, D. N. 1979. Nutritive Value of Thermoammoniated and Steam-Treated Maize Stover. I. Intake Digestibility and Nitrogen Retention. Anim. Feed. Sci. Technol., 4: 177-186
35. Ørskov, E.R. and McDonald, I. 1979. The Estimation of Protein Degradability in the Rumen from Incubation Measurements Weighted According to Rate of Passage. J. Agric. Sci., Camb., 92: 499–503.
36. Pascual, J. J., Fernandez, C., Diaz, J. R., Garces, C. and Rubert-Aleman, J. 2000. Voluntary Intake and In vivo Digestibility of Different Date-palm Fractions by Murciano-Granadina (Capra hircus). J. Arid Environ., 45: 183-189.
37. Pate, F. M. 1982. Value of Treating Bagasse with Steam under Pressure for Cattle Feed. Trop. Agric., 59: 293-297.
38. Popoff, T. and Theander, O. 1972. Formation of Aromatic Compounds from Carbohydrates. Carb. Res., 22: 135-149.
39. Puri, V. P. 1984. Effect of Crystallinity and Degree of Polymerization of Cellulose on Enzymatic Saccharification. Biotechnol. Bioeng., 26: 1219-1222.
40. Robertson, J. B. and Van Soest, P. J. 1981. The Detergent System of Analysis. In: “The Analysis of Dietary Fibre in Food”, (Eds.): James, W. P. T. and Theander, O.. Marcel Dekker, NY, 9: 123–158.
41. Sadeghi, M. H., Dashtizadeh, M., Kabirifard, A. M. and Zahedifar, M. 2001. Effect of Different Levels of Urea, Molasses and Duration of Treatment on Nutritive Value and Digestibility of Date Palm Branches: Final Report of Project. Agricultural and Natural Resources Research Center of Bushehr, Iran.
42. Ternud, I. E., Theander, O., Torneport, L. and Vallander, L. 1989. Changes in Chemical Composition of Steam-exploded Wheat Straw during Enzymic Treatment. Enz. Microb. Technol., 11: 500-506.
43. Theander, O. and Nelson, D. A. 1978. Aqueous, High-temperature Transformation of Carbohydrates Relative to Utilization of Biomass. Adv. Carb. Chem. Biochem., 46: 127-157.
44. Tipson, R. S. and Horton, D. 1988. Aqueous, High temperature Transformation of Carbohydrates Relative to Utilization of Biomass. Adv. Carb. Chem. Biochem., 46: 273 326.
45. Updegraff, D. M. 1969. Semi-micro Determination of Cellulose in Biological Materials. Anal. Biochem., 32: 420- 424.
46. Van Soest, P. J. 1982. Nutritional Ecology of the Ruminants. O and B Books Inc. Corvallis, OR, USA, 374 PP.
47. Van Soest, P. J. 1994. Nutrition Ecology of the Ruminants. 2nd Edition, Cornell University Press. PP. 156-176.
48. Van Soest, P. J., Robertson, J. B. and Lewis, B. A. 1991. Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition. J. Dairy Sci., 74: 3583-3597.
49. Wayman, M. and Chua, M. G. S. 1979. Characterization of Autohydrolysis Aspen (P. tremuloides) Lignins. Part 2. Alkaline Nitrobenzene Oxidation Studies of Extracted Autohydrolysis Lignin. Can. J. Chem., 57: 2599-2602.
50. Zahedifar, M. 1996. Novel Uses of Lignin and Hemicellulosic Sugars from Acid-Hydrolyzed Lignocellulosic Materials. PhD. Thesis, University of Aberdeen, Aberdeen. Scotland.
51. Zahedifar, M., Fazaeli, H., Norouzian, H. and Abbasi, A. 2004. Effect of Steam Pressure and Reaction Time on Chemical Composition and Bioavailability of Sugarcane Bagasse to Rumen Microbes. Proceedings of BSAS Annual Meeting, UK, 200 PP.
52. Zahedifar, M., Fazaeli, H., Nouruzian, H., and Abbasi, A. 2008. The Use of Steam Treatment for Improving the Nutritive Value of Roughages. First Phase: Determination of Optimum Conditions for Treating Sugar Cane Bagasse: Final Report of Research Project. Animal Science research Institute. Karaj, Iran.
Journal of Agricultural Science and Technology
Volume 16, Issue 4
July and August 2014
Pages 811-825