Seed Germination, Plant Establishment, and Yield of Sugar Beet Genotypes under Salinity Stress

Authors
S. Khayamim 1
R. Tavkol Afshari 2
S. Y. Sadeghian 1
K. Poustini 2
F. Roozbeh 3
Z. Abbasi 4
1 Sugar Beet Seed Research Institute, Mahdasht ave, Karaj, Islamic Republic of Iran.
2 Department of Agronomy and Plant Breeding, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Islamic Republic of Iran.
3 Sugar Beet Seed Research Instiute, Mahdasht ave, Karaj
4 Isfahan Agricultural Research and Natural Resources Institute, Isfahan, Islamic Republic of Iran.
Abstract
It is well known that sugar beet is sensitive to salinity stress at the germination stage. Three separate experiments were conducted to study the effects of salinity on seed germination, plant establishment, and yield of sugar beet genotypes for screening purposes. These included: (a) A laboratory study using four water salinity levels (with EC values < 0.1 as the control, 16, 20, and 24 dS m-1) with 20 sugar beet genotypes, which were evaluated in a factorial completely randomized design with four replications, and seedling characteristics were measured; (b) A greenhouse experiment where the same statistical design as the lab study was used for seed germination and establishment of 19 sugar beet materials, with irrigation water EC= 3 and 16 dS m-1; and (c) A field experiment that was carried out to study the response of nine selected genotypes to irrigation waters with EC= 4 and EC= 16 dS m-1, using a split plot design with three replications. Interaction effects of salinity and genotypes were statistically significant (α= 0.01) for percentage of germination, abnormal seedling, and root and hypocotyls lengths. Indeed, sugar beet germination decreased to 35% and dead seedlings increased to 80 % under salinity stress (EC= 16 dS m-1) in the greenhouse. Genotypes were ranked from tolerant to susceptible. The results of field experiment were consistent with that obtained in the greenhouse. It can be concluded that salt stress decreased seed germination and, later on, crop establishment by increasing dead seedlings; consequently, sugar beet yield decreased. It seems that establishment is more susceptible to salinity than germination. Root length and abnormal seedling are good indexes for screening sugar beet genotypes for salinity tolerance at the primary growth stages.

Keywords

1. Abdollahian, N. M., Sheikholeslami, R. and Babaee B. 2005. Technological Quantitative and Qualitative Expression and Explanation of Sugar Beet. Sugar Beet, 21: 101-104. (Persian with English Abstract)
2. AboKassem, E. ED. M. 2007. Effects of Salinity: Calcium Interaction on Growth and Nucleic Acid Metabolism in Five Spices of Chenopodiaceae. Turk. J. Bot., 31: 125-134.
3. Abrol, I. P., Yadav J. S. P. and Massoud F. I. 1988. Crops in Saline Soils. In: “Salt-Affected Soils and Their Management”. FAO Soils Bulletin, 39. [Online]. Available at: http://www.fao.org/docrep/x5871e/x5871e00.htm#Contents.
4. Al-Taisan, W. A. 2010. Comparative Effects of Drought and Salt Stresses on Germination and Seedling Growth of Pennisetum divisum (Gmel.) Henr. Amer. J. Appl. Sci., 7(5): 640-646.
5. Apostolides, G. and Goulas, C. 1998. Seed Crop Environment and Processing Effects on Sugar Beet (Beta vulgaris L.) Certified by Hybrid Variety Seed Quality. Seed Sci.Tech., 26: 223-235.
6. Ashraf, M. and Harris, P. J. C. 2004. Potential Biochemical Indicators of Salinity Tolerance in Plants. Plant Sci., 166: 3-16.
7. Dadkhah, A. 2011. Effect of Salinity on Growth and Leaf Photosynthesis of Two Sugar Beet (Beta vulagaris L.) Cultivars. J. Agr. Sci. Tech., 13: 1001-1012.
8. Dadkhah, A. and H. Grrifiths. 2006. The Effect of Salinity on Growth, Inorganic Ions and Dry Matter Partitioning in Sugar Beet Cultivars. J. Agr. Sci. Tech., 8: 199-210.
9. Doorenbaos, J. and Kassam, A. N. 1979. Yield Response to Water: Paper 33. FAO Irrigation and Drainage.
10. Duan, D., Liu, X., Ajmal Khan, M. and Gul, B. 2004. Effect of Salt and Water Stress on the Germination of Chenopodium glaucum L. Seed. Pak. J. Bot., 36(4): 793-800.
11. Durrant, M. J., Draycott, A. P. and Payne, P. A. 1974. Some Effect of Sodium Chloride on Germination and Seedling Growth of Sugar Beet. Annal. Bot., 38: 1045-1051.
12. Ebrahimian, H. R. and Ranji, Z. 2004 a. Preparing Tolerant Variety to Salt Stress with Diurnal Halfsib Selection: Final Report. Sugar Beet Seed Institute (SBSI), Karaj. (In Persian with English Abstract).
13. Ebrahimian, H. R. and Ranji, Z. 2004 b. Comparison of Salt Tolerant Variety 7233- 29*MST with Current Variety Sown in Esfahan: Final Report. Sugar Beet Seed Institute (SBSI), Karaj. (In Persian with English Abstract)
14. Ebrahimian, H. R., Ranji, Z., Rezaee, M. A. and Abbasi, Z. 2008. Screening Sugar Beet Genotypes under Salinity Stress in the Green House and Field Conditions. J. Sugar Beet, 24(1): 1-21. (in Persian with English Abstract)
15. Fotuhi, K., Mesbah, M., Sadeghian, S. Y., Ranji, Z. and Orazizadeh, M. R. 2006. Assessment Salt Tolerance in Sugar Beet Lines. J. Sugar Beet, 22(2): 1-18. (in Persian with English Abstract)
16. Habibi, D. 1993. Selection of Sugar Beet Tolerant Progenies to Drought and Salt Stress in Germination Phase. MSc. Thesis, Science and Research Branch, Islamic Azad University. (in Persian with English Abstract)
17. International Seed Testing Association. 1985. International Rules for Seed Testing: Rules 1985. Seed Sci. Tech., 13: 299-355
18. Jafarzadeh, A. A. and Aliasgharzadeh, N. 2007. Salinity and Salt Composition Effects on Seed Germination and Root Length of Four Sugar Beet Cultivars. Bio. Brati., 62(5): 562-564.
19. Jamil, M., Lee, D. B., Jung, K. Y., Ashraf, M. S., Lee, C. and Rha, E. S. 2006. Effect of Salt (NaCl) Stress on Germination and Early Seedling Growth of Four Vegetables Species. J. Cent. Europ. Agric., 7(2): 273-282.
20. Khayamim, S. 2010. Study of Physiological Parameters of Sugar Beet Lines in Salt Stress and Normal Conditions. PhD. Thesis, University of Tehran. (in Persian with English Abstract)
21. Mesbah, M., Yavari, N., Motahari, A. R., Arjmand, N. and Alimoradi, I. 1991. Results of Salt Tolerance Assessment in Different Sugar Beet Lines: Final Report. Sugar Beet Seed Institute (SBSI). (In Persian with English Abstract).
22. Mohammadian, R., Ghasemi Golozani, K., Moghaddam, M. and Sadeghian, S. Y. 1995. Effects of Seed Exhausted in Germination, Seedling and Establishment of Seven Sugar Beet Lines under Salt Stress. MSc. Thesis, Tabriz University. (in Persian with English Abstract)
23. Okcu, G., Kaya, M. D. and Atak, M. 2005. Effects of Salt and Drought Stresses on Germination and Seedling Growth of Pea (Pisum sativum L.). Turk J. Agric. Fores., 29: 237-242
24. Radaee, Z. 2009. Effects of Drought Stress in Farm and It’s Relation with Stress in Poly Ethylene Glycole for Screening Sugar Beet Genotypes. MSc. Thesis, University of Tehran. (in Persian with English Abstract)
25. Ramoliya, P. J. and Pandey A. N. 2002. Effect of Increasing Salt Concentration on Emergence Growth and Survival of Seedling of Salvadora oleoides. J. Arid Env., 51: 121-132.
26. Sadat Noori, S. A. and McNeilly, T. 2000. Assessment of Variability in Salt Tolerance Based on Seedling Growth in Triticum durum Desf. Genet. Resourc. Crop Evol., 47: 285-291.
27. Sadeghian, S. Y. and Khodaii, A. 1998. Diallel Cross Analysis of Seed Germination Traits in Sugar Beet. Euphytica, 103: 259-263.
28. Sadeghian, S. Y. and Lexander, K. 1993. Fatty Acid Composition of Plasma Membrane in Relation to Bolting in Sugar Beet. Flowering News, 16: 39-43
29. Sadeghian, S. Y. and Yavari, N. 2004. Effect of Water Deficit Stress on Germination and Early Seedling Growth in Sugar Beet. J. Agron. Crop Sci., 190: 138-144.
30. Shonjani, S. 2002. Salt Sensitivity of Rice, Maize, Sugar Beet and Cotton during Germination and Early Vegetative Growth. Inaugural Dissertation, Institute of Plant Nutrition, Justus Leibig University, 164 PP.
31. Yavari, N., Mesbah, M. and Ghaffari Jahromi, A. 2005. A Study on Advancing Treatment on Sugar Beet Embryo Culture in Salt Stress Conditions In vitro. J. Sugar Beet, 21(1): 87-97. (in Persian with English Abstract).
Journal of Agricultural Science and Technology
Volume 16, Issue 4
July and August 2014
Pages 779-790