An Integrated Approach Towards In vivo Control of Mushroom Weeds vis-à-vis Yield

Authors
M. R. Chakraborty 1
S. Ojha 1
R. N. Medda 1
N. C. Chatterjee 2
1 Department of Botany, Bankura Christian College, Bankura-722101, West Bengal, India.
2 Department of Botany, UGC Center for Advanced Study, The University of Burdwan, Burdwan – 713 104, West Bengal, India.
Abstract
Different competitor weed fungi Rhizopus stolonifer, Penicillium glabrum, Fusarium oxysporum and Coprinopsis kimurae were found to have associated with the fruit beds as constant contaminants of Pleurotus sajor-caju and Lentinula edodes. Several antagonists, plant extracts and chemicals were tested against the weed fungi to screen the most effective ones and to have an integrated combination for better management of the disease. Among the chemicals used, Baynate and Bavistin at the dose of 0.5% provided highly significant inhibition of all the competitors. Different plant extracts were treated against the weed fungi and it was found that garlic (Allium sativum) preparation exhibited the best result followed by Cycas revoluta and Azadirachta indica. Among the antagonists, Trichoderma viride was proved to be the most efficient one. Out of the various compatible combinations, the T. viride, garlicextract and Bavistin combination was found to be the most potent integrated combination providing more than 97% growth inhibition of the weed fungi in vitro. Under in vivo condition, biological efficiency of the combination of T. viride, T. hamatum, bavistin and garlic preparation was found to be significantly higher than untreated beds of both edible mushroom species.

Keywords

1. Amadioha, A. C. 2002. Fungitoxic Effect of Extracts of Azadirachta indica against Cochliobolus miyabeanus Causing Brown Spot Disease of Rich. Arch. Phytopatn. Pflanz., 35: 37-42.
2. Brahmachari, G. 2004. Neem, an Omnipotent Plant: A Retrospection. Chem. Biochem., 5: 408-421.
3. Chakraborty, M. R., Dutta, S., Ojha, S. and Chatterjee, N. C. 2004. Antagonistic Potential of Biocontrol Agents Against Botryodiplodia theobrome Causing Die-back of Bottle Brush (Callistemon citrinus). Acta Botanica Hungarica, 46(3-4): 279-286.
4. Chakraborty, M. R. and Chatterjee, N. C. 2008. Control of Fusariam Wilt of Solanum melongena by Trichoderma spp. Biologia Plantarum, 52(3): 582-586.
5. Cliquet, S. and Scheffer, R. J. 1996. Biological Control of Damping off Caused by Phytrium ultimum and Rhizoctonia solani using Trichoderma spp. Applied as Industrial Film Coating on Seeds. Europ. J. Pl. Pathol., 102(3): 247-255.
6. Jongman, M., Khonga, M., Khare, K. B. and Musyana, T. 2010. Effect of Seasonal Variation and Supplementation on Yield of Oyster Mushroom Cultivated on Indigenous Grasses in Botswana. Mushroom Res., 19(2): 54-61.
7. Mehrotra, R. S. and Aggarwal, A. 2010. Management of Plant Diseases. In: "Plant Pathology". 11th Reprint, Tata McGraw Hill Education Private Ltd., New Delhi, PP. 228-290.
8. Mishra, S. K. and Singh, R. P. 2006. Management of Dry Bubble Disease of Button Mushroom Using Botanicals and Bacterial Biocides. Indian J. Mush., 24(1): 31-39.
9. Mishra, S. K. and Singh, R. P. 2010. Interaction of Bioformulations and Pathogenic Fungi over Case Run and Yield of Agaricus bisporus (Lange) Imbach. Mushroom Res., 19 (1): 16-21.
10. Mondal, G., Srivastava, K. D. and Aggarwal, R. 1995. Antagonistic Effect of Trichoderma spp. On Ustilago segetum var. tritici and Their Compatibilities with Fungicides and Biocides. Indian Phytopath., 48(4): 466-470.
11. Pani, B. K. and Patra, A. K. 1997. Utilization of Some Phytoextracts for Control of Sclerotium rolfsii during Paddy Straw Mushroom Cultivation: A New Approach. Mushroom Res., 6(1): 37-42.
12. Patil, P. L. and Jadhav, S. W. 1994. Studies on Substrate Supplementation for Higher Yield of Oyster Mushroom. Mushroom Res., 3(1): 54.
13. Reyes, R. G., Lopez, L. L. M. A., Kalaw, S., Kikukawa, T. and Eguchi, F. 2009. Coprinus comatus, a Newly Domesticated Wild Nutricentical Mushroom in the Philippines. J. Agric. Technol., 5(2), 299-316.
14. Sangeetha, A., Kumar, P. S., Eswaramurthy, S. and Neelamegam, R. 2011. Effect of Different Leaf Extracts for Controlling Microflora Associated with Pleurotus citrinopileatus Cultivation. Mushroom Res., 20(1): 51-54.
15. Sharma, S. R. 1997. Plant Protection Operations in Mushroom Production. In: "Advances in Mushroom Biology and Production", (Eds.): Rai R. D., Dhar, B. L. and Verma R. N.. Mushroom Society of India, Solan, PP. 209-223.
16. Sharma, V. P. and Kumar, R. 2008. Management of Sepedonium Yellow Mould through Chemicals. Mushroom Res., 17(1): 19-23.
17. Singh, R. K. and Divedi, R. S. 1990. Fungicidal Properties of Neem and Blue Green against Sclerotium rolfsii Sacc. A Foot Rot Pathogen of Barley. Acta Bot. Indica. 18: 26-262.
18. Singh, A., Sharma, V. P., Kumar, S., Varshney, A and Singh, R. 2010. Prevalence of Competitor and Parasitic Moulds during Milky and White Button Mushroom Cultivation in Haryana. Mushroom Res., 19(1): 45-49.
19. Tewari, A. K. 2005. Pretreatment of Compost and Casing with Fungicides for the Management of Agaricus bisporus Moulds. Mushroom Res., 14(2): 72-75.
20. Vijoy, B. and Sohi, H.S. 1989. Studies on Fungal Competitors of Pleurotus sajor-caju (Fr.) Sig. Mushroom J. Tropics, 9: 29-35.
21. Wilson, C. L., Chalutz, A., Droby, E., Stevens, S., John, C. and Khan, Y. K. 1994. Potential of Induced Resistant to Control, Post Harvest Diseases of Fruit and Vegetables. Plant Dis., 78: 837-844.
Journal of Agricultural Science and Technology
Volume 15, Issue 4
July and August 2013
Pages 819-828