Effect of Different Storage Treatments on Physiology and ‎Postharvest Performance in Cut Scapes of Three Iris Species

Authors
Plant Physiology and Biochemistry Research Laboratory, Department of Botany, University of Kashmir, ‎Srinagar- 190006, India.‎
Abstract
The effect of different storage temperatures on senescence and postharvest performance in cut scapes of three Iris species (Iris germanica, Iris reticulata and Iris kashmiriana) was studied with the aim to develop a cost-effective storage protocol so as to bring out the transportation of these cut flowers. The scapes were subjected to two different storage treatments-dry storage and wet storage. For dry storage, the scapes were wrapped in moistened filter papers and kept at different storage temperatures [RT (20±2oC), 10and 5oC] for 72 hours. For wet storage, the scapes were kept in buckets containing distilled water (DW) and kept at different storage temperatures [RT (20±2oC), 10oC and 5oC] for 72 hours. After 72 hours storage, the scapes were transferred to flasks containing either DW or sucrose 0.15 M (SUC). Storage of buds for 72 hours at 5oC, followed by transfer to DW and SUC improved longevity in all the three species. Cold storage treatment before transfer to holding solutions improved floral diameter, membrane integrity and maintained higher fresh and dry mass of flowers, sugar content, and soluble proteins. In all the three species studied, enhanced vase life was found associated with the decrease in the total phenolic content of the perianth tissue. In conclusion, our results suggest that wet and dry storage of premature scapes of Irisesfor 72 hours at 5oC, and placing them in sucrose improves the cut flower performance and can be used as effective postharvest storage treatments for these beautiful cut flowers.

Keywords


1. Bunya-Atichart, K., Ketsa, S. and van Doorn, W.G. 2004. Postharvest Physiology of Curcuma alismatifolia Flowers. Postharvest Biol. Technol., 34: 219-226.
2. Celikel, F. G. and Reid, M. S. 2002. Storage Temperature Affects the Quality of Cut Flowers from the Asteraceae. Hort. Sci., 37: 148-150.
3. Cevallos, J. C. and Reid, M. S. 2001. Effect of Dry and Wet Storage at Different Temperatures on the Vase Life of Cut Flowers. Hort. Technol., 11: 199-202.
4. Finger, F. L. and Barbosa, J. G. 2006. Postharvest Physiology of Cut Flowers. In: "Advances in Postharvest Technologies for Horticultural Crops", (Eds.): Benkeblia, N. and Shiomi, N.. Research signpost, Kerala, PP. 373-393.
5. Gul, F., Tahir I. and Sultan, S. M. 2007. Effect of Storage Temperature on Postharvest Performance of Amaryllis belladonna L. cv. Rosea Scapes. J. Plant Biol., 34: 43-47.
6. Gul, F., Tahir I. and Sultan, S. M. 2007. Effect of storage temperature on postharvest performance of Amaryllis belladonna L. cv. Rosea Scapes. J. Plant Biol., 34: 43-47.
7. Hoeberichts, F. A., van Doorn, W. G., Vorst, O., Hall, R. D. and van Wordragen, M. F. 2007. Sucrose Prevents Upregulation of Senescence-Associated Genes in Carnation Petals. J Exp. Bot., 58: 2873-2885.
8. Ichimura, K., Kohata, K. and Goto, R. 2000. Soluble Carbohydrates in Delphinium and Their Influence on Sepal Abscission in Cut Flowers. Physiol. Plantarum, 108: 307-313.
9. Ichimura, K., Kojima, K. and Goto, R. 1999. Effect of Temperature , 8-hydroxyquinoline Sulphate and Sucrose on the Vase Life of Cut Rose Flowers. Postharvest Biol. Technol., 15: 33-40.
10. Joyce, D. C., Meara, S. A., Hetherington, S. E. and Jones, P. 2000. Effects of Cold Storage on Cut Grevillea ‘Sylvia’ Inflorescences. Postharvest Biol. Technol., 18: 49-56.
11. Leonard, R. T., Nell, T. A., Suzuki, A., Barrett, J. E. and Clark, D. G. 2001. Evaluation of Long Term Transport of Colombian Grown Cut Flowers. Acta Hortic., 543: 293-297.
12. Lowry, O. H., Rosenbrough, N. J., Farr, A. L. and Randall, R. J, 1951. Protein Measurement with Folin Phenol Reagent. J. Biol. Chem., 193: 265-275.
13. Mwangi, M., Chatterjee, S. R. and Bhattacharjee, S. K. 2003. Changes in the Biochemical Constituents of “Golden Gate” Cut Rose Petals as Affected by Precooling with Ice Cold Water Spray, Pulsing and Packaging. J. Plant Biol., 30: 95-97.
14. Nelson, N. 1944. A Photometric Adaptation of the Somogyi’s Method for the Determination of Glucose. J. Biol. Chem., 153: 375-380.
15. Pun, U. K. and Ichimura, K., 2003. Role of Sugars in Senescence and Biosynthesis of Ethylene in Cut Flowers; JARQ, 4: 219-224.
16. Rudnicki, R. M., Nowak, J. and Goszczynska, D. M. 1991. Cold Storage and Transportation Conditions for Cut Flowers Cuttings and Potted Plants. Acta Hortic., 298: 225-236.
17. Shahri, W. and Tahir, I. 2011. An Effective Storage Protocol for Improving the Postharvest Performance in Cut Spikes of Consolida ajacis Nieuwl cv. Violet Blue. Sci. Hortic., 129: 154-158.
18. Shahri, W., Tahir, I. and Gul, F. 2009. Effect of Dry and Wet Storage at Cool Temperatures on Postharvest Performance of Consolida ajacis cv. Violet Blue Spikes. J. Plant Biol., 36: 5-9.
19. Shahri, W., Tahir, I., Islam, S. T. and Ahmad, M. 2010. Response of Some Ornamental Flowers of Family Ranunculaceae to Sucrose Feeding. Afr. J. Plant Sci., 4: 346-352.
20. Swain, T. and Hillis, W. E. 1959. The Phenolic Constituents of Prunus domestica L. the Quantitative Analysis of Phenolic Constituents. J. Sci. Fd. Agr., 10: 63-68.
21. van Doorn, W. G. and deWitte, Y. 1991. Effect of Dry Storage on Bacterial Counts in Stems of Cut Rose Flowers. Physiol. Plantarum, 31: 15-22.