Aflatoxins in Dried Figs in Turkey: A Comparative Survey on the Exported and Locally Consumed Dried Figs for Assessment of Exposure

Authors
Department of Food Engineering, Faculty of Agriculture, Adnan Menderes University, 09100, Aydin, Turkey
Abstract
Dried figs collected from various exporting companies (2,461 samples) and local stores (219 samples) were analyzed using reverse-phase high-performance liquid chromatography (RP-HPLC) to determine and compare the incidence of aflatoxins, the effectiveness of the regulation limits and monitoring system to select non-compliant samples and the mean daily aflatoxin exposures. The incidence rates of aflatoxin contamination were higher in the domestic samples (47.5%) than in the samples intended for export (23.6%). According to the European regulation limits (2 ng g-1 for aflatoxin B1 and 4 ng g-1 for total aflatoxin; sum of B1+B2+G1+G2), non-compliant samples were 6 and 24.2% of the dried figs for export and domestic market, respectively. The respective rates of 2.2 and 16.4% were obtained with the national limit. The dietary intakes of aflatoxins through the consumption of dried figs were calculated as 1.27 and 0.2 ng kg-1 body weight×day for domestic market and export, respectively. Dried figs contaminated with high levels of aflatoxins can lead to acute and chronic human toxicities. Reducing contamination and exposure to an acceptable level by the implementation of strict periodic monitoring and application of effective new prevention measures might also help to decrease the significant health and economic risks in exported and domestic commodities.

Keywords


1. Aegean Exporters’ Association. 2009. Available from: http://www.egeliihracatcilar.com/asp/content.asp?ms=1&id=0
2. Bircan, C. 2009. Comparison of Homogenization Techniques and Incidence of Aflatoxin Contamination in DriedFigs for Export. Food Addit. Contam., 2(B): 171-177.
3. Boudra, H., Le Bars, J., Le Bars, P. and Dupuy, J. 1994. Time of Aspergillus flavus Infection and Aflatoxin Formation in Ripening of Figs. Mycopathol., 127: 29-33.
4. Boyacioglu, D. and Gonul, M. 1990. Survey of Aflatoxin Contamination of Dried Figs Grown in Turkey in 1986. Food Addit. Contam., 7: 235-237.
5. Buchanan, J. R., Sommer, N. F. and Fortlage, R. J. 1975. Aspergillus flavus Infection and Aflatoxin Production in Fig Fruits. Appl. Microbiol., 30: 238-241.
6. Castegnaro, M. and McGregor, D. 1998. Carcinogenic Risk Assessment of Mycotoxins. Rev. Med. Vet-Toulouse, 149: 671-678.
7. Chu, F. S. 1997. Mode of Action of Mycotoxins and Related Compounds. Adv. Appl. Microbiol., 40: 352-357.
8. Coffey, R., Cummins, E. and Ward, S. 2009. Exposure Assessment of Mycotoxins in Dairy Milk. Food Control, 20: 239-249.
9. Del Pozo, A. C. and Lopez, P. 2007. Management of Hepatocellular carcinoma. Clin. Liver Dis., 11: 305-321.
10. Doster, M. A., Michailides, T. J. and Morgan, D. P. 1996. Aspergillus Species and Mycotoxins in Figs from California Orchards. Plant Dis., 80: 484-489.
11. European Commission, Commission Regulation (EC) no 1881. 2006. Setting Maximum Levels for Certain Contaminants in Foodstuffs. Offic. J. European :union:., 364(L): 5-24.
12. European Commission, Commission Regulation (EC) No. 2174. 2003. Maximum Levels for Aflatoxin B1 and Aflatoxin Total in Certain Foodstuffs. Offic. J. European :union:., 326:12-15.
13. Fooladi, M. H. and Farahnaky, A. 2003. Aflatoxin Removal from Pistachio Nuts by Natural Natrolite. J. Food Sci., 68: 1225-1228.
14. Galvano, F., Galofaro, V. and Galvano, G. 1996. Occurrence and Stability of Aflatoxin M1 in Milk and Milk Products: A Worldwide Review. J. Food Prot., 59: 1079-1090.
15. Gunal, N. 2008. Turk Dunyasinda Incir Kulturu. Turkish Studies-international Periodical for the Languages, Literature and History of Turkish or Turkic, 3: 561-581.
16. Iamanaka, B. T., Menezes, H. C., Vicente, E., Leite, R. S. F. and Taniwaki, M. H. 2007. Aflatoxigenic Fungi and Aflatoxins Occurrence in Sultanas and Dried Figs Commercialized in Brazil. Food Control, 18: 454-457.
17. International Agency for Research on Cancer (IARC). 1993. Evaluation of Carcinogenic Risks of Chemical to Humans. "Some Naturally-occurring Substances. Food Items and Constituents". Heterocyclic Aromatic Amines and Mycotoxins Monographs, Lyon, France, Vol. 56.
18. Lafont, P., Siriwardana, M. and Lafont, J. 1989. Genotoxicity of Hydroxy-aflatoxins M1 and M2. Microbiol. Aliment. Nutr., 7: 1-8.
19. Luchese R. H. and Harrigan, W. F. 1993. Biosynthesis of Aflatoxin: the Role of Nutritional Factors. J. Appl. Bacteriol., 72: 5-14.
20. Mahoney, N. E. and Rodriguez, S. B. 1996. Aflatoxin Variability in Pistachios. Appl. Environ. Microbiol., 2: 1197-1202.
21. Pariza, W. M. 1996. Toxic Substances, in Food Chemistry. (Ed.): Fennema O. R., and Dekker, M.. New York.825-840
22. Payne, G. A. and Hagler, W. M. 1983. Effect of Specific Amino Acids on Growth and Aflatoxin Production by Aspergillus parasiticus and Aspergillus flavus in Defined Media. Appl. Environ. Microbiol., 46: 805-812.
23. Pildain, M. B., Vaamonde, G. and Cabrala, D. 2004. Analysis of Population Structure of Aspergilus flavus from Peanut Based on Vegetative Compatibility, Geographic Origin, Mycotoxin and Sclerotia Production. Int. J. Food Microbiol., 93: 31-40.
24. Rapid Alert System for Food and Feed (Rasff). 2009. Weekly Reports for 2003, 2004, 2005, 2006, 2007, 2008. Available from: http://ec.europa.eu/food/food/rapidalert/index_en.htm.
25. Reddy, T. V., Viswanathan, L. and Venkitasubramanian, T. A. 1971. High Aflatoxin Production on a Chemically Defined Medium. Appl. Microbiol., 22: 393-396.
26. Senyuva, H. Z., Gilbert, J. and Ulken, U. 2007. Aflatoxins in Turkish Dried Figs Intended for Export to the European :union:. J. Food Prot., 70: 1029-1032.
27. Shephard, S. G. 2008. Risk Assessment of Aflatoxins in Food in Africa. Food Addit. Contam., 25: 1246-1256.
28. Stenier, W, Rieker, R. H. and Battaglia, R. 1988. Aflatoxin Contamination in Dried Figs: Distribution and Association with Fluorescence. J. Agric. Food Chem., 36: 88-91.
29. Sun, C. A. and Chen, C. J. 2003. Aflatoxin-induced Hepatocarcinogenesis: Epidemiological Evidence and Mechanistic Considerations. J. Med. Sci., 23: 311-318.
30. Turk Gida Kodeksi Teblig, Gida Maddelerindeki Bulaşanlarin Maksimum Limitleri Hakkinda Tebliğde Değişiklik Yapilmasi Hakkinda Tebliğ (Tebliğ no: 2009/22). 2009. Resmi Gazete, 16.02.2009, Sayi: 27143.
31. Van Egmond, H. P. 1989. Current Situation on Regulations for Mycotoxins. Overview of Tolerances and Status of Standard Methods for Sampling and Analysis. Food Addit. Contam., 6: 139-188.
32. Williams, H.J., Phillips, D. T., Jolly, E. P., Stiles, K.J., Jolly, M. C. and Aggarwal, D. 2004. Human Aflatoxicosis in Developing Countries: A Review of Toxicology, Exposure, Potential Health Consequences, and Interventions. Am. J. Clin. Nutr., 80: 1106-1122.