Abundance and Diversity of AM Fungi across a Gradient of Land Use Intensity and Their Seasonal Variations in Niligiri Biosphere of the Western Ghats, India

Authors
R. Lakshmipathy 1
A. N. Balakrishna 2
D. J. Bagyaraj 2
1 Post Harvest Technology Center, Agricultural College Campus, Bapatla-522 101, India.
2 Department of Agricultural Microbiology, University of Agricultural Sciences, GKVK, Bangalore-560 065, India.
Abstract
The impact of land use intensity on the abundance and diversity of arbuscular mycorrhizal fungi (AMF) was investigated at six land use types viz., natural forest, grassland, acacia plantations, cardamom plantations, coffee plantations and paddy fields in the Niligiri Biosphere of the Western Ghats in South India. There was no significant difference in AMF root colonization in different land use types during pre-monsoon but there was a significant difference in AMF root infection ratings between different land use types during post-monsoon season, where it was higher in natural forests and grasslands. The AMF spore density and infective propagules were significantly higher in grasslands and acacia plantations compared to all other land use types during both seasons. Except for paddy fields, the spore density and number of infective propagules were higher in post-monsoon season compared to pre-monsoon season in other land use types. The numbers of AMF species identified were 56 during pre-monsoon and 67 species during post-monsoon season suggesting seasonal variations in diversity. During both seasons Glomus fasciculatum was recorded in maximum number of sampling points across the landscape followed by G. geosporum during pre-monsoon and G. mosseae during post-monsoon season. The species diversity was highest in natural forests and grasslands as compared to other land use types in both seasons. The species richness index for AMF was highest in natural forests and least in paddy fields during both study periods. The sand content, bulk density, total N, organic C, alkaline and acid phosphatases positively correlated with AMF activity while clay, silt, K, total P and available P were negatively correlated.

Keywords

1. Anderson, J. M. and Ingram, J. S. I. 1993. Tropical Soil Biology and Fertility: A Handbook of Methods. 2nd Edition, CAB International, Wallingford, 55pp.
2. Attitchabi, J., Coyne, D., Hountondji, F., Lawouin, L., Wiemken, A. and Oehl, F. 2008, Arbuscular Mycorrhizal Fungi in the ‘Yam Belt’ of West Africa. African J. Biotechnol., 56: 256-268.
3. Augé, R. M. 2004. Water Relations, Drought and Vesicular-arbuscular Mycorrhizal Symbiosis. Mycorrhiza, 11: 3–42.
4. Bagyaraj, D. J. and Manjunath, A. 1980. Selection of Suitable Host for Mass Production of VA Mycorrhizal Inoculation. Plant Soil, 55: 495-498.
5. Bagyaraj, D. J. and Varma, A. 1995. Interactions between Arbuscular Mycorrhizal Fungi and Plants: Their Importance in Sustainable Agriculture in Arid and Semiarid Tropics. Adv. Microbiol. Ecol., 14: 119-142.
6. Bagyaraj, D. J. and Sturmer, S. L. 2008. Arbuscular Mycorrhizal Fungi (AMF). In: "A Handbook of Tropical Soil Biology: Sampling and Characterization of Belowground Biodiversity", (Eds.): Moreira, F. M. S., Huising, J. E. and Bignell, D. E.. Earthscan Publication, London, PP. 131- 147.
7. Balakrishna, A. N., Lakshmipathy, R., Sudhir, K. and Bagyaraj, D. J. 2001. Effect of Long Term Application of Inorganic Fertilizers on Native VA Mycorrhizal Fungi and Soil Microbial Biomass in a Finger Millet-maize-fallow Rotation. J.Soil Biol.Ecol., 21: 1-6.
8. Bhardwaj, S., Dudeja, S. S. and Khurana, A. L. 1997. Distribution of Vesicular-arbuscular Mycorrhizal Fungi in the Natural Ecosystem. Folia-Microbiol., 42: 589-594.
9. Blaszkowski, J. 1994. Comparative Studies on the Occurrence of Arbuscular Fungi and Mycorrhizae (Glomales) in Cultivated and Uncultivated Soils of Poland. Acta Mycol., 28: 93-140.
10. Boddington, C. L. and Dodd, J. C. 2000. The Effect of Agricultural Practices on the Development of Indigenous Arbuscular Mycorrhizal Fungi. II. Studies in Experimental Microcosms. Plant Soil, 218: 145-157.
11. Byra Reddy, M. S., Krishna Naik, L., Bhaskar,V. and Bagyaraj, D. J. 1994. Status of Mycorrhizal Association in Some Tropical Tree Species. J. Soil Biol. Ecol., 14: 51-54.
12. Carpenter, F. L., Palacios, M. S., Gonzalez, Q. E. and Schroeder, M. 2001. Land Use and Erosion of a Costa Rican Ultisol Affect Soil Chemistry, Mycorrhizal Fungi and Early Regeneration. For. Ecol. Manage., 144: 1-17.
13. Doss, D. D. and Bagyaraj, D. J. 2001. Ecosystem Dynamics of Mycorrhizae. In: "Innovative Approaches in Microbiology", (Eds.): Maheswari, D. K. and Dubey, R. C.. Bishen Singh Mahendra Pal Singh Publication, Dehra Dun, India, PP. 115-129
14. Eivazi, F. and Tabatabai, M. A. 1977. Phosphatases in Soils. Soil. Biol. Biochem., 9: 167-172.
15. Fischer, C. R., Janos, D. P., Perry, D. A. Linderman, R. G. and Sollins, P. 1994. Mycorrhiza Inoculum Potentials in Tropical Secondary Sucession. Biotropica, 26: 369-377.
16. Gemma, J. N. and Koske, R. E. 1988. Seasonal Variation in Spore Abundance and Dormancy of Gigaspora gigantea and in Mycorrhizal Inoculum Potential of a Dune Soil. Mycologia 80: 211-216.
17. Gerdemann, J. W. and Nicolson, T. H. 1963. Spores of Mycorrhizal Endogone Species Extracted from the Soil by Wet Sieving and Decanting. Trans. Br. Mycol. Soc., 46: 235-244.
18. Giovannetti, M. and Mosse, B. 1980. An Evaluation of Techniques to Measure Vesicular Arbuscular Infection in Roots. New Phytol., 84: 489-500.
19. Harinikumar, K. M. and Bagyaraj, D. J. 1988. The Effect of Season on VA Mycorrhiza of Leucaena and Mango in Semi-arid Tropic. Arid Soil Res. Rehabil., 7: 139-143.
20. Harinikumar, K. M., Bagyaraj, D. J. and Mallesha, B. C. 1990. Effect of Intercropping and Organic Soil Amendments on Native VA Mycorrhiza in Semi-arid Tropics. Arid Soil Res. Rehabil., 4: 193-197.
21. Harinikumar, K. M. and Bagyaraj, D. J. 1989. Effect of Cropping Sequences, Fertilizers and Farm Yard Manure on VA Mycorrhizal Fungi. Biol. Fert. Soils, 7: 173-175.
22. Hayman, D. S. 1978. Mycorrhizal Population of Sown Pastures and Native Vegetation in Otago, New Zealand. NZ. J. Agric. Res., 21: 271-276.
23. Hayman, D. S. 1980. VA Mycorrhiza and Crop Production. Nature, 287: 487-488.
24. Helgason, T., Merryweather, J. W., Young, J. P. and Fitter, A. H. 2007. Specificity and Resilience in the Arbuscular Mycorrhizal Fungi of a Natural Woodland Community. J. Ecol., 95: 623–630.
25. Hibbett, D. S., Binder, M., Bischoff, J. F., Blackwell, M. and Cannon, P. F. 2007. A Higher Level Phylogenetic Classification of the Fungi. Mycol. Res., 111: 509-514.
26. Husband, R., Herre, E. A., Turner, S. L. Gallery, R. and Young, J. P. W. 2002. Molecular Diversity of Arbuscular Mycorrhizal Fungi and Pattern of Host Association over Time and Space in a Tropical Forest. Molecular Ecol., 11: 2669-2678.
27. Jackson, M. L. 1973. Soil Chemical Analysis. Prentice Hall Pvt. Ltd., New Delhi, India, PP.105-110.
28. Janos, D. P. and Read, D. J. 1992. Heterogenity and Scale in Tropical Vesicular-arbuscular Mycorrhiza Formation. In: "Mycorrhiza in Ecosystems", (Eds.): Fitter, A. H. and Alexander, I. J.. CAB international US, London, PP. 276-282.
29. Janos, D. P. 1980. Mycorrhizae Influence Tropical Succession. Biotropica, 12: 56-64.
30. Johnson, N. C. and Wedin, D. A. 1997. Soil Carbon, Nutrients, and Mycorrhizae during Conversion of Dry Tropical Forest to Grassland. Ecol. Applications, 7: 171-182.
31. Clark, R. B. and Zeto, S. K. 2000. Mineral Acquisition by Arbuscular Mycorrhizal Plants. J. Plant. Nutr., 23: 867–902.
32. Koide, R. T. 1991. Nutrient Supply, Nutrient Demand and Plant Response to Mycorrhizal infection. New Phytol. 117: 365-386.
33. Kormanik, P. P., Bryan, W. C. and Schultz, R. C. 1980. Increasing Endomycorrhizal Fungus Inoculum in Forest Nursery Soil with Cover Crops. South. J. Appl. For., 4: 151-153.
34. Krebs, C. J. 1989, Ecological Methodology. Harper Collins Publishers, 58 PP.
35. Krishna, K. R. and Bagyaraj, D. J. 1982. Effect of Vesicular-arbuscular Mycorrhiza and Soluble Phosphate on Abelmoscus esculentus (L.) Moench. Plant Soil, 64: 209-213.
36. Kruckelman, H.W. 1975. Effects of Fertilizers, Soils, Soil Tillage and Plant Species on the Frequency of Endogone Chlamadospores and Mycorrhizal Infection in Arable Soils. In: "Endomycorrhizas", (Eds.): Sanders, F. E., Mosse, B. and Tinker, P. B.. Academic Press. London, PP. 511-525.
37. Lakshmipathy, R., Sumana, D. A., Balakrishna, A. N., Bagyaraj, D. J. and Kumar, D. P. 2004. Evaluation, Grafting Success and Field Establishment of Cashew Rootstock as Influenced by VAM Fungi. Ind. J. Expt. Biol., 42: 1132-1135
38. Lakshmipathy. R., Balakrishna, G. and Bagyaraj, D. J. 2003. VA Mycorhizal Colonization Pattern in RET Medicinal Plants (Mammea suriga, Saraca asoca, Garcinia spp., and Embelia ribes and Calamus sp.) in Different Parts of Karnataka. Asian J. Microbiol. Biotech. Env. Sci., 5: 505-508.
39. Lakshmipathy. R., Balakrishna G., Chandrika, K. and Bagyaraj, D. J. 2002. VA Mycorrhizal Colonization Pattern in Hemidesmus indicus and Dioscorea bulbifera in Different Parts of Karnataka. Giobios, 29: 209-212.
40. Lovelock, C. E., Andersen, K. and Morton, J. B. 2003. Arbuscular Mycorrhizal Communities in Tropical Forests Are Affected by Host Tree Species and Environment. Oceologia, 132: 268-279.
41. Mallesha, B. C. and Bagyaraj, D. J. 1991. Season Favouring Sporulation of VA Mycorrhizal Fungi in Cardamom Plantations. Soil Boil. Ecol., 11: 75-78.
42. Mendez, A. B., Scervine, J. M. and Fodeas, A. M. 2001. Arbuscular Mycorrhizal Population Associated with Natural and Cultivated Vegetation on a Site of Buenos Aires Province, Argentina. Biol. Fertil. Soils, 33: 373-381.
43. Mervin, H. D. and Peech, M. 1951. Exchangebility of Soil Potassium in the Sand, Silt and Clay Fractions as Influenced by the Nature of the Complementary Exchangeable Cation. Proc. Soil Sci. Soc. Amer., 15: 125-129.
44. Mohan, C. 2003. Mycorrhizae in Forest Plantations: Association, Diversity and Exploitation in Planting Stock Improvement: KFRI Research Report-252. Peechi.
45. Molina, R. J., Trappe, J. M. and Strickler, G. S. 1978. Mycorrhizal Fungi Associated with Festuca in the Western United States and Canada. Can. J. Bot., 56: 1691-1695.
46. Morton, J. B. and Benny, G. L. 1990. Revised Classification of Arbuscular Mycorrhizal Fungi (Zygomycetes): A New Order, Glomales, Two New Families, Acaulosporaceae and Gigasporaceae with an Emendation of Glomaceae. Mycotaxon, 37: 471-491.
47. Morton, J. B. 1990. Species and Clones of Arbuscular Mycorrhizal Fungi (Glomales zygomycetes): Their Role in Macro and Micro–evolutionary Process. Mycotaxon, 37: 493–515.
48. Muthukumar, T. and Udayan, K. 2000. Arbuscular Mycorrhizas of Plants Prowing in the Western Ghats Region, Southern India. Mycorrhiza, 9: 297-313.
49. Muthukumar, T. K. V. and Manian, S. 1993. Vesicular Arbuscular Mycorrhizal Fungi in Western Ghats. Ind. Bot., 10: 79-83.
50. Myers, N., Mittermeir, R. A., Mittermeir, C. G., da Gustavo, A. B. and Kent, J. 2000. Biodiversity Hotspots for Conservation Priorities. Nature, 403: 853-858.
51. Oehl, F., Sieverding, E., Ineichen, L., Mader, P., Boller, T. and Wienmken, A. 2003. Impact of Land Use Intensity on the Species Diversity of Arbuscular Myocrrhial Fungi in Agroecosystems of Central Europe. Appl. Env. Microbiol., 69: 2816-2824.
52. Oehl, F., Sieverding, E., Goto, T. B., Palenzeula, J., Ferrol, N., da Silva, G. A. and de Souza, F. A. 2009. The Fairytale of the Different Morphological Identification of AMF: Recent Advances in the Area. In: "Beyond the Roots". Belo Horizonte, Brazil, 2009, 17 PP.
53. Palenzuela, J., Ferrol, N., Boller, T., Azcon-Aguilar, C. and Oehl, E. 2008, Otospora bareai: A New Fungal Species in the Glomeromycetes from a Dolomitic Shrubland in the Natural Park of Sierra de Baza (Granada, Spain). Mycologia, 100: 296-305.
54. Pande, M. and Tarafdar, J. C. 2004. Arbuscular Mycorrhizal Fungal Diversity in Neem Based Agroforestry Systems in Rajasthan. App. Soil Ecol., 26: 233-241.
55. Patrícia, L.L, Stürmer S. L. and Siqueira, J. O. 2009. Occurrence and Diversity of Arbuscular Mycorrhizal Fungi in Trap Cultures from Soils under Different Land Use Systems in the Amazon, Brazil. Brazilian J. Microbiol., 40: 111-121.
56. Phillips, J. M. and Hayman, D. S. 1970. Improved Procedures for Clearing and Staining Parasites and Vesicular Arbuscular Mycorrhizal Fungi for Rapid Assessment of Infection. Trans. Br. Mycol. Soc., 55: 158-161.
57. Picone, C. M. 2000. Diversity and Abundance of Arbuscular Mycorrhizal Fungus Spores in Tropical Forest and Pasture. Biotropica, 32: 734-750.
58. Pirozynski, K. A. and Dalphe, Y. 1989. Geological History of the Glomaceae with Particular Reference to Mycorrhizal Symbiosis. Symbiosis, 7: 1–36.
59. Porter, W. M. 1979. The Most Probable Number Method for Enumerating Infective Propagules of Vesicular Arbuscular Mycorrhizal Fungi in Soil. Aus. J. Soil Res., 17: 515-519.
60. Porter, W. M., Robson, A. D. and Abbott, L. K. 1987. Field Survey of the Distribution of Vesicular-arbuscular Mycorrhizal Fungi in Relation to Soil pH. J. App. Ecol., 24: 659-662.
61. Sanders, I. R. and Fitter, A. H. 1992. Evidence for Differential Responses between Host-fungus Combinations of Vesicular-arbuscular Mycorrhizas from a Grassland. Mycol. Res., 96: 415-419.
62. Santhaguru, K., Ponmalar, S. B. G. and Karunakaran, R. 1995. Vesicular-arbuscular Mycorrhizae in Tree-legumes and Its Rhizospheric Soils in Alagar Hills. Ind. For., 121: 817-823.
63. Schenck, N. C. and Kinloch, R. A. 1980. Incidence of Mycorrhizal Fungi on Six Field Crops in Monoculture on a Newly Cleared Woodland Site. Mycologia, 72: 229-443.
64. Schenk, N. C. and Perez, Y. 1990. Manual for Identification of VA Mycorrhizal Fungi. 3rd Edition, Synergistic Publications, Gainesville, Florida, USA, P. 1-142.
65. Shi, Z., Y., Wang, F. and WeI Y. L. 2007. Natural Forest and Forest Plantation Affect Diversity of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Diptorocarpaceae. American-Eurasian J. Agric. Environ. Sci., 2: 411-416.
66. Sieverding, E. and Leihner, D. 1984. Effect of Herbicides on Population Dynamics of VA Mycorrhiza with Cassava. Angew Botanic. 58: 283-294.
67. Sieverding, E. 1991. Vesicular Arbuscular Mycorrhiza Management in Tropical Agro-ecosystems. Technical Corporation, Federal Republic of Germany, Eschbom, PP. 307-311.
68. Sieverding, E. and Oehl, F. 2006. Revision of Entrophospora and Description of Kuklospora and Intraspora: Two New Genera in the Arbuscular Mycorrhizal Glomeromycetes. J. App. Bot. Food Quality, 80: 69-81.
69. Snedecor, S. W. and Cochram, W. G. 1968. Statistical Methods. Oxford and IBH Publication, New Delhi, 124 PP.
70. Tommerup, I. C. and Sivasithamparam, K. 1990. Zygospores and Asexual Spores of Gigaspora decipiens: An Arbuscular Mycorrhizal Fungus. Mycol. Res., 94: 897-900.
71. Vasanthakrishna, M., Muthanna, M. B. and Bagyaraj, D. J. 1994. Succession of Vesicular Arbuscular Mycorrhizal Fungi Associated with Casuarina equisetifolia L. Ann. For., 2: 123-126.
72. Walker, C., Vestberg, M., Demircik, F., Stockinger, H., Saito, M., Sawaki, H., Nishmura, I. and Schufler, A. 2007a. Molecular Phylogeny and New Taxa in the Archaeosporales. Mycol. Res. 111: 3-49.
73. Walker, C., Vestberg, M., Demircik, F., Stockinger, H., Saito, M., Sawaki, H., Nishmura, I. and Schufler, A. 2007b. Molecular Phylogeny and New Taxa in the Archaeosporales. Mycol. Res. 111: 137-153.
Journal of Agricultural Science and Technology
Volume 14, Issue 4
July and August 2012
Pages 903-918