Comparison of Energy Reserves in Prediapause and Diapausing Adult Sunn Pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae)

Authors
A. Amiri 1
A. R. Bandani 2
1 Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
2 Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Islamic Republic of Iran.
Abstract
In this study, the energy reserves of prediapause and diapausing adult bugs were examined using colorimetric biochemical techniques to determine carbohydrates, lipids, glycogen, and protein content. To this end, 45-day-old bugs were obtained from three different sources: laboratory colony, cold-stored insects, or natural habitat. The results showed that prediapause males and females had significantly lower lipids than laboratory cultured bugs, those were collected in natural habitat, and cold-treated diapausing insects. In contrast to lipids, carbohydrate and glycogen contents were significantly higher in prediapause males and females than in diapausing insects. Glycogen content in laboratory-reared females and males were significantly higher than in their counterparts from natural habitat. In conclusion, Sunn pest energy reserves change from prediapause to diapause phases. Prediapause Sunn pest bugs accumulate lipids for their metabolic needs during diapause and for post-diapause functions that include dispersal and reproduction.

Keywords

1. Adkisson, P. L., Bell, R. A. and Wellso, S. G. 1963. Environmental Factors Controlling the Induction of Diapause in the Pink Bollworm, Pectinophara gossypiella (Saunders). J. Insect Physiol., 9: 299-310.
2. Allahyari, M., Bandani, A. R. and Rezaei, M. H. 2010. Subcellular Fractionation of Midgut Cells of the Sunn Pest Eurygaster integriceps (Hemiptera: Scutelleridae): Enzyme Markers of Microvillar and Perimicrovillar Membranes. J. Insect Physiol., 56(7): 710-717.
3. Bailey, E. 1975. Biochemistry of Insect Flight. Part 2. Fuel Supply. In: "Insect Biochemistry and Function", (Eds.): Candy, D. J. and Kilby, B. A.. Chapman and Hall, London, PP. 89-176.
4. Bandani, A. R., Kazzazi, M. and Mehrabadi, M. 2009. Purification and Characterization of Midgut Alpha-amylases of Eurygaster integriceps. J. Entomol. Sci., 12: 25-32.
5. Bashan, M., Akbas, H. and Yurdakoc, K. 2002. Phospholipid and Triacylglycerol Fatty Acid Composition of Major Life Stages of Sunn Pest, Eurygaster integriceps (Heteroptera: Scutelleridae). Comp. Biochem. Physiol., 132: 375-380.
6. Beenakkers, A. M., Van Der Host, T. H. D. J. and Van Marrewijk, W. J. A. 1984. Insect Flight Muscle Metabolism. Insect Biochem., 14(3): 243-260.
7. Bowen, M. F. 1992. Patterns of Sugar Feeding in Diapausing and Nondiapausing Culex pipiens (Diptera: Culicidae) Females. J. Med. Entomol., 29: 843-849.
8. Bradford, M. M. 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Proteins Utilizing the Principle of Protein Dye Binding. Anal. Biochem., 72: 248-254.
9. Briegel, H. 1989. Inability of Diapausing Culex pipiens (Diptera: Culicidae) to Use Blood for Producing Lipid Reserves for Overwintering Survival. J. Med. Entomol., 26: 318-326.
10. Briegel, H. 1990. Metabolic Relationship between Female Body Size, Reserves, and Fecundity of Aedes aegypti. J. Insect Physiol., 36: 165-172.
11. Critchley, B. R. 1998. Literature Review of Sunn Pest Eurygaster integriceps Put. (Hemiptera: Scutelleridae). Crop Protection, 4: 271-287.
12. Danks, H. V. 1987. Insect Dormancy: An Ecological Perspective. Biological Survey of Canada, Ottawa, 439 PP.
13. Danks, H. V. 2000. Dehydration in Dormant Insects. J. Insect Physiol., 46: 837-852.
14. De Kort C. A. D. 1990. 35 Years of Diapauses Research with the Colorado Potato Beetle. Entomol. Exp. Appl., 56: 1-13.
15. Ellers, J. and Van Alphen, J. M. 2002. A Trade-off between Diapause Duration and Fitness in Female Parasitoids. Ecol. Entomol., 27: 279-284.
16. Emerson, K. J., Bradshaw, W. E. and Holzapfel, C. M. 2009. Complications of Complexity: Integrating Environmental, Genetic and Hormonal Control of Insect Diapause. TIG, 25(5): 217-225.
17. Hahn, D. A. and Denlinger, D. L. 2007. Meeting the Energetic Demands of Insect Diapause: Nutrient Storage and Utilization. J. Insect Physiol., 53: 760-773.
18. Hahn, D. A. and Denlinger, D. L. 2011. Energetics of Insect Diapause. Annu. Rev. Entomol., 56: 103–21.
19. Hines, W. J. W. and Smith, M. J. H. 1963. Some Aspects of the Intermediary Metabolism in the Desert Locust, Schistocerca gregaria Forskal. J. Insect Physiol., 9: 463-468.
20. Inagaki, S. and Yamashita, O. 1986. Metabolic Shift from Lipogenesis to Glycogenesis in the Last Instar Larval Fat Body of the Silkworm, Bombyx mori. Insect Biochem., 16: 327- 31.
21. Mitchell, C. J. and Briegel, H. 1989. Inability of Diapausing Culex pipiens (Diptera: Culicidae) to Use Blood for Producing Lipid Reserves for Overwinter Survival. J. Med. Entomol., 26: 318–326.
22. Niaqi E. N., Olembo, N. K. and Pearson, D. J. 1992. Proline Transport by Tsetse Fly Glossina morsitans Flight Muscle Mitochondria. Comp. Biochem. Physiol. B., 102(3): 579-584.
23. Paulian, F. and Popov, C. 1980. Sunn Pest or Cereal Pest, In: "Wheat", (Ed.): Hafliger, E.. Ciba-Geigy, Basel, PP. 69–74.
24. Radjabi G. H. 2000. Ecology of Cereal's Sunn Pests in Iran. Agricultural Research Education and Extension Organisation Press, Iran, 343 PP.
25. Rambabu, J. P. and Rao, M. B. 1994. Effect of Organochlorine and Three Organophosphate Pesticides on Glucose, Glycogen, Lipid and Protein Contents in Tissues of the Freshwater Snail Bellamya dissimilis (Muller). Bull. Environ. Contam. Toxicol., 53: 142-148.
26. Sancho, E., Ferrando, M. D., Fernandez, C. and Andreu, E. 1998. Liver Energy Metabolism of Anguilla anguilla after Exposure to Fenitrothion. Ecotoxicol. Environ. Saf., 41: 168-175.
27. Shinyaeva, L. I. 1980. Spermatogenesis in the Noxious Pentatomid (Eurygaster integriceps) during the Period of Prediapause and Diapause Formation. Zool. Zh., 59(7): 1025-1032.
28. Steele, J. E. 1985. Control of Metabolic Processes. In: "Comprehensive Journal of Insect Physiology, Biochemistry, and Pharmacology", (Eds.): Kekurt G. A. and Gilbert, L. I.. Pergamon Press, Oxford, 8: 99-146.
29. Storey, K. B. 1997. Organic Solutes in Freezing Tolerance. Comp. Biochem. Physiol., 117(A): 319-326.
30. Venkatesh, K. and Morrison, P. E. 1980. Studies of Weight Changes and Amount of Food Ingested by the Stable Fly, Stomoxys calcitrans (Diptera: Muscidae). Can. Entomol., 112: 141-49.
31. Withers, P.C. 1992. Comparative Animal Physiology. Saunders College Pub., New York, 949 PP.
32. Worland, M. R., Grubor-Lajsic, G. and Montiel, P. O. 1998. Partial Desiccation Induced by Sub-zero Temperatures as a Component of the Survival Strategy of the Arctic Collembolan Onychiurus arcticus (Tullberg). J. Insect Physiol., 44: 211-219.
33. Yuval, B., Kaspi, R., Shloush, S. and Warburg, M. S. 1998. Nutritional Reserves Regulate Male Participation in Mediterranean Fruit Fly Leks. Ecol. Entomol., 23: 211-215.
34. Zhou, G., Pennington, J. E. and Wells, M. A. 2004. Utilization of Pre-existing Energy Stores of Female Aedes aegypti Mosquitoes during the First Gonotrophic Cycle. Insect Biochem. Mol. Biol., 34: 919-925.
Journal of Agricultural Science and Technology
Volume 15, Issue 3
May and June 2013
Pages 435-444