Evaluation of Pakistani Elite Wheat Germplasm for T1BL.1RS Chromosome Translocation

Authors
R. Tahir 1
H. Bux 2
A. G. Kazi 3
A. Rasheed 4
A. A. Napar 4
S. U. Ajmal 1
A. Mujeeb-Kazi 5
1 Pir Mehr Ali Shah (PMAS)-Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan.
2 Institute of Plant Sciences, University of Sindh Jamshoro-76080, Sindh, Pakistan.
3 Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Science and Technology (NUST) Islamabad 44000, Islamabad, Pakistan.
4 Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i.Azam University, Islamabad, Pakistan.
5 National Institute of Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
Abstract
Rye (Secale cereale) chromosome 1RS harbors multiple genes including Lr26, Sr31, Yr9 and Pm8 conferring disease resistance and tolerance to abiotic stresses. The introgression of the rye 1R chromosome short arm has enormously contributed to increase of genetic diversity in wheat. Utilization of such translocations in breeding programs demands identification of wheat germplasm possessing the wheat-alien chromosome translocation. This study was designed to screen a set of 102 Pakistani wheat cultivars and candidate lines to identify the rye T1BL.1RS translocation, using cytological, biochemical, and molecular techniques. Results revealed that 12 out of the 40 wheat cultivars were found to have this alien introgression. In the National Uniform Wheat Yield Trials (NUWYT) group, 10 of 23 entries of the rainfed category were identified as carrying 1BL.1RS translocation, while 4 out of 39 genotypes were present in the irrigated category of both NUWYT crop seasons. The valuable information generated can be useful in the crop improvement programs for the production of germplasm possessing T1BL.1RS translocation, in order to enhance the genetic variability in local wheat cultivars and, also, avoid the preponderance of T1BL.1RS candidates.

Keywords

1. Baum, M. and Appels, R. 1991. The Cytogenetic and Molecular Architecture of Chromosome 1R - One of the Most Widely Utilized Sources of Alien Chromatin in Wheat Cultivars. Chromosoma, 101: 1-10.
2. Bennett, F.G.A. 1984. Resistance to Powdery Mildew in Wheat: A Review of Its Use in Agriculture and Breeding Programs. Plant Pathol., 33: 279-300.
3. Berzonsky, W. A. and Francki, M.G. 1999. Biochemical, Molecular, and Cytogenetic Technologies for Characterizing 1RS in Wheat: A Review. Euphytica, 108: 1-19.
4. Bibi, A., Rasheed, A., Kazi, A.G., Mahmood, T., Ajmal, S. and Mujeeb-Kazi, A. 2012. High-molecular-weight (HMW) Glutenin Subunit Composition of the Elite-II Synthetic Hexaploid Wheat Subset (Triticum turgidum × Aegilops tauschii; 2n= 6x= 42; AABBDD). Plant Genetic Resource, 10(1):1-4.
5. Carver, B. F. and Rayburn A. L. 1994. Comparison of Related Wheat Stocks Possessing 1B or 1RS.1BL Chromosomes: Agronomic Performance. Crop Sci., 34: 1505-1510.
6. Friebe, B., Jiang J., Raupp W. J., McIntosh, R. A. and Gill, B. S. 1996. Characterization of Wheat-Alien Translocations Conferring Resistance to Diseases and Pests: Current Status. Euphytica, 91: 59–87.
7. He, Z. H., Liu, L., Xia, X. C., Liu, J. J. and Pena, R. J. 2005. Composition of HMW and LMW Glutenin Subunits and Their Effects on Dough Properties, Pan Bread, and Noodle Quality of Chinese Bread Wheats. Cereal Chem., 42: 345-350.
8. Heun, M. and Fischbeck, G. 1987. Identification of Wheat Powdery Mildew Resistance Genes by Analyzing Host-Pathogen Interactions. Plant Breed., 98: 124-129.
9. Hoffmann, B. 2008. Alteration of Drought Tolerance of Winter Wheat Caused by Translocation of Rye Chromosome Segment 1RS. Cereal Res. Comm., 36: 269-278.
10. Jahan, Q., Ter-Kuile, N., Hashmi, N., Aslam, M., Vahidy, A. A. and Mujeeb-Kazi, A. 1990. The Status of the 1B/1R Translocation Chromosome in Some Released Wheat Cultivars and the 1989 Candidate Cultivars of Pakistan. Pak. J. Bot., 22: 1-10.
11. Jiang, J., Friebe, B. and Gill B. S. 1994. Chromosome Painting of Amigo Wheat. Theor. Appl. Genet., 89: 811-813.
12. Ko, J. M., Seo, B. B., Suh, D. Y., Do, G. S., Park, D. S. and Kwack, Y. H. 2002. Production of a New Wheat Line Possessing the 1BL.1RS Wheat-rye Translocation Derived from Korean Rye Cultivar Paldanghomil. Theor Appl Genet., 104(2-3):171-176.
13. Koebner, R. M. D. and Shepherd, K. W. 1986. Controlled Introgression to Wheat of Genes from Rye Chromosome Arm 1RS by Induction of Allosyndesis. Theor. Appl. Genet., 73: 197-208.
14. Koebner, R. M. D. 1995. Generation of PCR Markers for the Detection of RyeChromatin in a Wheat Background. Theor. Appl. Genet., 90: 740-745.
15. Kumar, S., Kumar, N., Balyan, H. S. and Gupta, P. K. 2003. 1BL.1RS Translocation in Some Indian Bread Wheat Genotypes and Strategies for Its Use in Future Wheat Breeding. Caryologia, 56:1, 23-30.
16. Kumlay, A. M., Baenziger, P. S. K., Gill, K. S., Shelton, D. R., Graybosch, R. A., Lukaszewski, A. J. and Wesenberg, D. M. 2003. Understanding the Effect of Rye Chromatin in Bread Wheat. Crop Sci., 43: 1643-1651.
17. Landjeva, S., Korzun, V., Tsanev, V., Vladova, R. and Ganeva, G. 2006. Distribution of the Wheat-rye Translocation 1RS.1BL among Bread Wheat Cultivars of Bulgaria. Plant Breed., 125: 102-104.
18. Lutz, J., Limpert, E., Bartos, P. and Zeller, F. J. 1992. Identification of Powdery Mildew Resistance Genes in Common Wheat (Triticum aestivum L.) I. Czechoslovakian Cultivars. Plant Breed., 108: 33-39.
19. Marais, G. F., Horn, M. and Du, Toit F. 1994. Intergeneric Transfer (Rye to Wheat) of a Gene (s) for Russian Wheat Aphid Resistance. Plant Breed., 113: 265-271.
20. Martin, T. J., Harvey, T. L. and Livers, R. W. 1976. Resistance to Wheat Streak Mosaic Virus and Its Vector, Aceriatulipae. Phytopath., 66: 346-349.
21. Mcintosh, R. A., Hart, G. E. and Gale, M. D. 1993. Catalogue of Gene Symbols for Wheat Glutenin and the Characterization of 1R (1B) Substitution and 1BL/1RS Translocation Lines. Euphytica, 33: 3-8.
22. Merker, A. 1982. “Veery”: A CIMMYT Spring Wheat with 1B/1R Chromosome Translocation. Cereal Res. Commun., 10: 105-106.
23. Mirzaghaderi, G., Hassani, H. S. and Karimzadeh, G. 2010. C-banded Karyotype of Thinopyrum bessarabicum and Identification of Its Chromosomes in Wheat Background. Genet. Resour. Crop Evol., 57: 319-324.
24. Mirzaghaderi, G., Zeinali, G., Rafiepour, M. and Karimzadeh, G. 2011. Wheat-rye Translocation in Iranian Bread Wheats and Their Ion Distribution in Response to Salinity Stress. J. Agr. Sci. Tech. (JAST), 13: 1163-1172.
25. Mettin, D., Blüthne,r W. D. and Schlegel, R. 1973. Additional Evidence on Spontaneous 1B/1R Wheat-Rye Substitutions and Translocations. Proc. 4th Int. Wheat Genet. Symp., University of Missouri, Columbia, Missouri, USA, PP.179-184.
26. Moreno-Sevilla, B., Baenziger, P. S., Peterson, C. J., Graybosch, R. A., Mcvey, D. V. 1995. The 1BL/1RS Translocation: Agronomic Performance of F3-derived Lines from a Winter Wheat Cross. Crop Sci., 35: 1051-1055.
27. Mujeeb-Kazi, A. 1982. CIMMYT Report on Wheat Improvement. CIMMYT, Mexico, PP. 78-87.
28. Mujeeb-Kazi, A. and Miranda, J. L. 1985. Enhanced Resolution of Somatic Chromosome Constrictions as an Aid to Identifying Intergenetic Hybrids among Some Triticeae. Cytologia, 50: 701-709.
29. Mujeeb-Kazi, A. and Rajaram, S. 2002. Transferring Alien Genes from Related Species and Genera for Wheat Improvement. Bread Wheat Improvement and Production. FAO, Rome, PP. 199-215.
30. Mujeeb-Kazi, A, William, M. D. H. M. and Islam-Faridi, M. N. 1996. Homozygous 1B and 1BL/1RS Chromosome Substitutions in Triticum aestivum and T. turgidum Cultivars. Cytologia, 61: 147-154.
31. Mujeeb-Kazi, A, Jahan, Q. and Vahidy, A. A. 1994. Application of a Somatic and Meiotic Cytological Technique to Diverse Plant Genera and Species in the Triticeae. Pak. J. Bot., 26: 353-366.
32. Mujeeb-Kazi, A., A. G. Kazi, I. Dundas, A. Rasheed, P. Chen, M. Kishi, D. Bonnett, R. R. C. Wang, H. Bux and S. Farrukh. 2013. Genetic Diversity for Wheat Improvement as a Conduit to Food Security. Adv. Agron., 122, 179-257.
33. Rajaram, S, Mann, C. E., Ortiz-Ferrara, G. and Mujeeb-Kazi, A. 1983. Adaptation, Stability and High Yield Potential of Certain 1B/1R CIMMYT Wheats. Proc. Sixth Intl. Wheat Genet. Symp., Kyoto, Japan, PP. 613-621.
34. Rabinovich, S. V. 1998. Importance of Wheat-rye Translocations for Breeding Modern Cultivars of Triticum aestivum L. Euphytica 100: 323-340.
35. Rayburn, A. L. and Carver, B. F. 1988. Cytological Identification of 1B/1R Wheat-rye Translocation in Winter Wheat Breeding Lines. Euphytica, 38: 327-340.
36. Schlegel, R. and Meinel, A. 1994. A Quantitative Trait Locus (QTL) on Chromosome arm 1RS of Rye and Its Effect on Yield Performance of Hexaploid Wheat. Cereal Res. Commun., 22: 7-13.
37. Singh, N. K., Shepherd, K. W. and Mcintosh, R. A. 1990. Linkage Mapping of Genes for Resistance to Leaf, Stem and Stripe Rusts and ω-secalins on the Short Arm of Rye Chromosome 1R. Theor. Appl. Genet., 80: 609-616.
38. Tabibzadeh, N., Karimzadeh, G. and Naghavi, M. R. 2013. Distribution of 1AL.1RS and 1BL.1RS Wheat-rye Translocations in Iranian Wheat, Using PCR Based Markers and SDS PAGE. Cereal Res. Commun., 41(3): 1-10.
39. Trethowan, R. M. and Mujeeb-Kazi, A. 2008. Novel Germplasm Resources for Improving Environmental Stress Tolerance of Hexaploid Wheat. Crop Sci., 48: 1255-1265.
40. Villareal, R. L., Mujeeb-Kazi, A., Rajaram, S. and Del Toro, E. 1994. Associated Effects of Chromosome 1B/1R Translocation on Agronomic Traits in Hexaploid Wheat. Breed. Sci., 4: 7-11.
41. Villareal, R. L., Rajaram, S., Mujeeb-Kazi, A., Del Toro, E. 1991. The Effect of Chromosome1B/1R Translocation on the Yield Potential of Certain Spring Wheats (Triticum aestivum L.). Plant Breed., 106: 77-81.
42. Villareal, R. L., Bañuelos, O. and Mujeeb-Kazi, A. 1997. Agronomic Performance of Related Durum Wheat (Triticum turgidum L.) Stocks Possessing the Chromosome Substitution T1BL.1RS. Crop Sci., 37: 1735-1740.
43. Villareal, R. L., Bañuelos, O., Mujeeb-Kazi, A. and Rajaram, S. 1998. Agronomic Performance of Chromosomes 1B and T1BL.1RS Near-isolines in the Spring Bread Wheat Seri M82. Euphytica, 103: 195-202.
44. Weining, B. and Langridge, P. 1991. Identification and Mapping Polymorphism in Cereals Based on PCR. Theor. Appl. Genet., 82: 209-216.
45. Weng, Y., Azhaguvel, P., Devkota, R. N. and Rudd, J. C. 2007. PCR-based Markers for Detection of Different Sources of 1AL.1RS and 1BL.1RS Wheat-rye Translocations in Wheat Background. Plant Breed., 126: 482- 486.
46. William, M. D. H. M. and Mujeeb-Kazi, A. 1993. Rapid Detection of 1B, 1BL/1RS Heterozygotes in the Development of Homozygous 1BL/1RS Translocation Stocks of Triticum turgidum (2n= 4x= 28). Genome, 36: 1088-1091.
47. Zeller, F. J. and Hsam, S. L. K. 1984. Broadening the Genetic Variability of Cultivated Wheat by Utilizing Rye Chromatin. In: “Proc. Sixth Intl. Wheat. Genet. Symp.”, (Ed.): Sakamoto, S.. Kyoto University, Japan., PP. 161-173.
48. Zeller, F. 1973. 1B/1R Wheat-rye Chromosome Substitutions and Translocations. Proc. Fourth Intl. Wheat Genet. Symp., Columbia, Missouri, USA., PP. 209-222.
49. Zuniga, J., Soto, B. and Campos, H. 2008. Using a Gel-free PCR-ELISA for the Molecular Identification of Wheat Genotypes Carrying Wheat Rye Translocations. Plant Breed., 127: 15-19.
Journal of Agricultural Science and Technology
Volume 16, Issue 2
March and April 2014
Pages 421-432