Phenolic Content, Antiradical, Antioxidant, and Antibacterial Properties of Hawthorn (Crataegus elbursensis) Seed and Pulp Extract

Authors
S. Salmanian
A. R. Sadeghi Mahoonak
M. Alami
M. Ghorbani
Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Islamic Republic of Iran.
Abstract
The hawthorn fruits have been used as food and medicine for centuries. In the present study, pulp and seed extract of Crataegus elbursensis Rech. F. fruits belonging to the family Rosaceae and native of northern part of Iran were evaluated for the polyphenol contents, antiradical, antioxidant, and antibacterial activities. The total phenolic, flavonoid, and anthocyanin contents of methanolic pulp extract were found to be more than those of methanolic seed extract. The DPPH radical scavenging, iron (III) reducing capacity, and total antioxidant activity of the extracts depended on concentration. A 200 μg ml-1 of C. elbursensis pulp and seed extract and butylated hydroxytoluene (BHT) exhibited 82.13, 83.47, and 85.44% inhibition, respectively. However, effect of the extracts in the total antioxidant activity and reducing power were not significantly as good as BHT. In addition, the results showed that both pulp and seed extract had inhibitory activity against the four bacteria tested, with the pulp extract showing more activity than the seed extract. Also, phenolic acids were identified by RP-HPLC and chlorogenic acid was the predominant phenolics in the samples. In conclusion, our results showed that C. elbursensis pulp and seed extract had strong antioxidant and antibacterial activities, which were correlated with its high level of polyphenols.

Keywords

1. Ait-Ouazzou, A., Lorán, S., Arakrak, A., Laglaoui, A., Rota, C., Herrera, A., Pagán, R. and Conchello, P. 2012. Evaluation of the Chemical Composition and Antimicrobial Activity of Mentha pulegium, Juniperus phoenicea, and Cyperus longus Essential Oils from Morocco. Food Res. Int., 45: 313–319.
2. Arslan, R., Bor, Z., Bektas, N., Meriçli, A. H. and Ozturk, Y. 2011. Antithrombotic Effects of Ethanol Extract of Crataegus orientalis in the Carrageenan-induced Mice Tail Thrombosis Model. Throm. Res., 127: 210-213.
3. Arabshahi, S. and Urooj, A. 2007. Antioxidant Properties of Various Solvent Extracts of Mulberry (Morus indica L.) Leaves. Food Chem., 102:1233-1240.
4. Bajpai, V. K., Al-Reza, S. M., Choi, U. K., Lee, J. H. and Kang, S. C. 2009. Chemical Composition, Antibacterial and Antioxidant Activities of Leaf Essential Oil and Extracts of Metasequioa glyptostroboides Miki ex Hu. Food Chem. Toxicol., 47: 1876–1883.
5. Cai, Y. Z., Sun, M., Xing, J., Luo, Q. and Corke, H. 2006. Structure-radical Scavenging Activity Relationships of Phenolic Compounds from Traditional Chinese Medicinal Plants. Life Sci., 78: 2872-2888.
6. Chang, C., Yang, M., Wen, H. and Chern, J. 2002. Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Food Drug Anal., 10: 178-182.
7. Cui, T.; Li, J.Z., Kayahara, H., Ma, L., Wu, L.X. and Nakamura, K. 2006. Quantifica tion of the Polyphenols and Triterpene Acids in Chinese Hawthorn Fruit by High-Performance Liquid Chromatography. J. Agric. Food Chem., 54, 4574–4581.
8. Ebrahimzadeh, M. A. and Bahramian, F. 2009. Antioxidant Activity of Crataegus pentagyna subsp. elburensis Fruits Extracts Used in Traditional Medicine in Iran. Pak. J. Biol. Sci., 12(5): 413-419.
9. Froehlicher, T., Hennebelle, T., Martin-Nizard, F., Cleenewerck, P., Hilbert, J. L., Trotin, F. and Grec, S. 2009. Phenolic Profiles and Antioxidative Effects of Hawthorn Cell Suspensions, Fresh Fruits, and Medicinal Dried Parts. Food Chem., 115: 897–903.
10. Gutiérrez-Larraínzar, M., Rúa, J., Caro, Castro, C. D., Arriaga, D. D., García-Armesto, M. R. and Valle, P.D. 2012. Evaluation of Antimicrobial and Antioxidant Activities of Natural Phenolic Compounds against Foodborne Pathogens and Spoilage Bacteria. Food Control., 26: 555-563.
11. Jurikova, T., Sochor, J., Rop, O., Mlcek, J., Balla, S., Szekeres, L., Adam, V. and Kizek, R. 2012. Review Polyphenolic Profile and Biological Activity of Chinese Hawthorn (Crataegus pinnatifida BUNGE) Fruits. Molecules, 17: 14490-14509.
12. Kalemba, D. and Kunicka, A. 2003. Antibacterial and antifungal properties of essential oils. Curr. Med. Chem., 10(10): 813–829.
13. Kil, H. Y., Seong, E. S., Ghimire, B. K., Chung, I. M., Kwon, S. S., Goh, E. J., Heo, K., Kim, M. J., Lim, J. D., Lee, D. and Yu, C. Y. 2009. Antioxidant and Antimicrobial Activities of Crude Sorghum Extract. Food Chem., 115: 1234–1239.
14. Koba, K., Matsuoka, A., Osada, K. and Huang, Y. S. 2007. Effect of Loquat (Eriobotrya japonica) Extracts on LDL Oxidation. Food Chem., 104: 308–316.
15. Kwok, C. Y., Wong, C. N. Y., Mabel, Y. C. Y., Yu, P. H. F., Au, A. L. S., Poon, C. C. W., Seto, S. W., Lam, T. Y., Kwan, Y. W. and Chan, S. W. 2010. Consumption of Dried Fruit of Crataegus pinnatifida (Hawthorn) Suppresses High-cholesterol Diet-induced Hypercholesterolemia in Rats. J. Funct. Foods, 2: 179 – 186.
16. Lin, J. Y. and Tang, C. Y. 2007. Determination of Total Phenolic and Flavonoid Contents in Selected Fruits and Vegetables, as well as Their Stimulatory Effects on Mouse Splenocyte Proliferation. Food Chem., 101: 140–147.
17. Liu, P., Yang, B. and Kallio, H. 2010 a. Characterization of Phenolic Compounds in Chinese Hawthorn (Crataegus pinnatifida Bge. var. major) Fruit by High Performance Liquid Chromatography–electrospray Ionization Mass Spectrometry. Food Chem., 121: 1188–1197.
18. Liu, P. Z., Kallio, H., Lu, D. G., Zhou, C. S., Ou, S. Y. and Yang, B. R. 2010 b. Acids, Sugars, and Sugar Alcohols in Chinese Hawthorn (Crataegus spp.) Fruits. J. Agric. Food Chem., 58: 1012–1019.
19. Meot-Duros, L., Floch, G. L. and Magné, C. 2008. Radical Scavenging, Antioxidant and Antimicrobial Activities of Halophytic Species. J. Ethnopharmacol., 116: 258–262.
20. Muanda, F. N., Soulimani, R., Diop, B. and Dicko, A. 2011. Study on Chemical Composition and Biological Activities of Essential Oil and Extracts from stevia rebaudiana Bertoni Leaves. LWT - Food Sci. Technol., 44: 1865-1872.
21. Negi, P. S. 2012. Review Plant Extracts for the Control of Bacterial Growth: Efficacy, Stability and Safety Issues for Food Application. Int. J. Food Microbiol., 156: 7–17.
22. NCCLS: National Committee for Clinical Laboratory Standards. 2001. Performance Standards for Anti-microbial Susceptibility Testing: Eleventh Informational Supplement. Document M 100-S11. National Committee for Clinical Laboratory Standard, Wayne, PA, USA.
23. Pande, G. and Akoh, C. C. 2010. Organic Acids, Antioxidant Capacity, Phenolic Content and Lipid Characterization of Georgia-grown Underutilized Fruit Crops. Food Chem., 120: 1067–1075.
24. Petti, S. and Scully, C. 2009. Polyphenols, Oral Health and Disease: A Review. J. Dent., 37: 413–423.
25. Prieto, P., Pineda, M. and Aguilar, M. 1999. Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E. Anal. Biochem., 269:337–341.
26. Rabiei, Kh., Bekhradnia, S., Nabavi, S.M., Nabavi, S. F., and Ebrahimzadeh, M. A. 2012. Antioxidant Activity of Polyphenol and Ultrasonic Extracts from Fruits of Crataegus pentagyna subsp. elburensis. Nat. Prod. Res., 26(24): 2353–2357.
27. Sadeghi, N., Jannat, B., Oveisi, M. R., Hajimahmoodi, M. and Photovat, M. 2009. Antioxidant Activity of Iranian Pomegranate (Punica granatum L.) Seed Extracts. J. Agr. Sci. Tech., 11: 633-638.
28. Shimada, K., Fujikawa, K., Yahara, K. and Nakamura, T. 1992. Antioxidative Properties of Xanthin on Autoxidation of Soybean Oil in Cyclodextrin Emulsion. J. Agr. Food Chem., 40: 945-948.
29. Shukla, Sh., Mehta, A., Bajpai, V. K. and Shukla, S. 2009. In vitro Antioxidant Activity and Total Phenolic Content of Ethanolic Leaf Extract of Stevia rebaudiana Bert. Food Chem. Toxicol., 47: 2338–2343.
30. Silici, S., Sagdic, O. and Ekici, L. 2010. Total Phenolic Content, Antiradical, Antioxidant and Antimicrobial Activities of Rhododendron Honeys. Food Chem., 121: 238–243.
31. Slinkard, K. and Singleton, V. L. 1977. Total Phenol Analysis; Automation and Comparison with Manual Methods. Am. J. Enol. Viticult., 28: 49–55.
32. Sun, L., Zhang, J., Lu, X., Zhang, L. and Zhang, Y. 2011. Evaluation to the Antioxidant Activity of Total Flavonoids Extract from Persimmon (Diospyros kaki L.) Leaves. Food Chem. Toxicol., 49: 2689-2696.
33. Tajkarimi, M. M., Ibrahim, S.A. Cliver, D. O. 2010. Review Antimicrobial Herb and Spice Compounds in Food. Food Control, 21: 1199–1218.
34. Urbonaviciute, A., Jakstas, V., Kornygova, O., Janulis, V. and Maruska, A. 2006. Capillary Electrophoretic Analysis of Flavonoids in Single-styled Hawthorn (Crataegus monogyna Jacq.) Ethanolic Extracts. J. Chromatogr. A, 1112: 339–344.
35. Weerakkody, N. S., Caffin, N., Turner, M. S. and Dykes, G. A. 2010. In vitro Antimicrobial Activity of Less-utilized Spice and Herb Extracts against Selected Food-borne Bacteria. Food Control, 21: 1408–1414.
36. Yasoubi, P., Barzegar, M., Sahari, M. A. and Azizi, M. H. 2007. Total Phenolic Contents and Antioxidant Activity of Pomegranate (Punica granatum L.) Peel Extracts. J. Agric. Sci. Technol., 9: 35-42.
37. Yildirim, A., Mavi, A. and Kara, A. A. 2001. Determination of Antioxidant and Antimicrobial Activities of Rumex crispus L. Extracts. J. Agr. Food Chem., 49: 4083-4089.
Journal of Agricultural Science and Technology
Volume 16, Issue 2
March and April 2014
Pages 343-354