Morpho-pomological and Chemical Diversity of Pomegranate Accessions Grown in Eastern Mediterranean Region of Turkey

Authors
O. Caliskan
S. Bayazit
Department of Horticulture, Faculty of Agriculture, University of Mustafa Kemal, 31034, Antakya, Hatay, Turkey.
Abstract
Selecting within local pomegranate accessions is the main method used to identify new cultivars. Total of 76 pomegranate accessions from Hatay, Turkey, were collected and their morpho-pomological and chemical characteristics were determined. The results showed that there was significant diversity among the accessions in terms of fruit quality parameters. Several accessions were notable for their various characteristics. For example, ‘Ekşi 5’, ‘Ekşiliknar’, ‘Kara Mehmet 1’, ‘Lifani 5’ and ‘Ekşi 3’ accessions could be used for extracted aril and juice as they had dark red arils and juice, good taste, and large arils. In addition, the sweet accessions ‘Tatlı 3’, ‘Tatlı 13’, and ‘Tatlı 16’ with soft seeds, rosy peel, and red aril colors were very promising for fresh consumption. Our study demonstrated that there was great morpho-pomological variability among the local pomegranates grown in eastern Mediterranean region of Turkey, making them a valuable genetic source for incorporation into potential breeding programs, especially for different fruit quality characteristics.

Keywords

1. Bell, C. and Hawthorne, S. 2008. Ellagic Acid, Pomegranate and Prostate Cancer Mini Review. J. Pharm. Pharmacol., 60:139-144.
2. Bellini, E. and Giordani, E. 1998. Descriptor List for Pomegranate (Punica granatum L.). Project on "Minor Fruit Tree Species Conservation": RESGEN29. Horticulture Department, University of Florence, Italy.
3. Berinyuy, J. E., Fontem, D. A., Focho, D. A. and Schippers, R. R. 2002. Morphological Diversity of Solanum scabrum Accessions in Cameroon. Plant Genet. Res. News., 131: 42-48.
4. Camara, M. M., Diez, C. and Torija, M. E. 1996. Free Sugar Determination by HPLC in Pineapple Product. Unters Z. Lebensm. Forsh, 202: 233-237.
5. Cheng, G. W. and Bren, P. J. 1991. Activity of Phenylalanine Ammonia Lyase (PAL) and Concentrations of Anthocyanins and Phenolics in Developing Strawberry Fruit. J. Am. Soc. Hort. Sci., 116: 865-869.
6. Cristofori, V., Caruso, D. Latini, G., Dell’agli, M., Cammilli, C., Rugini, E., Bignami, C. and Muleo, R. 2011. Fruit Quality of Italian Pomegranate (Punica granatum L.) Autochthonous Varieties. Eur. Food Res. Technol., 232: 397-403.
7. Drogoudi, P. D., Tsipouridis, C. and Michailidis, Z. 2005. Physical and Chemical Characteristics of Pomegranates. Hort. Sci., 40: 1200-1203.
8. Durgaç, C., Özgen, M., Şimşek, Ö., Aka Kaçar, Y. A., Kıyga, Y., Çelebi, S., Gündüz, K. and Serçe, S. 2008. Molecular and Pomological Diversity among Pomegranate (Punica granatum L.) Cultivars in Eastern Mediterranean Region of Turkey. Afr. J. Biotechnol., 7: 1294–1301.
9. Ercisli, S., Kafkas, E., Orhan, E., Kafkas, S., Doğan, Y. and Esitken, A. 2011. Genetic Characterization of Pomegranate (Punica granatum L.) Genotypes by AFLP Markers. Biol. Res., 44: 345-350.
10. Esquinas Alcázar, J. 2005. Protecting Crop Genetic Diversity for Food Security: Political, Ethical and Technical Challenges. Nat. Rev. Genet., 6: 946-953.
11. Ferrara, G., Cavoski, I., Pacifico, A., Tedone, L. and Mondelli, D. 2011. Morpho-pomological and Chemical Characterization of Pomegranates (Punica granatum L.) Genotypes in Apulia Region, Southeastern Italy. Sci. Hort., 130: 599-606.
12. Hasnaoui, N., Mars, M., Chibani, J. and Trifi, M. 2010a. Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L.) as Revealed by RAPD Fingerprints. Diversity, 2: 107-114.
13. Hasnaoui, N., Buonamici, A., Sebastiani, F., Mars, M., Trifi, M. and Vendramin, G. G. 2010b. Development and Characterization of SSR Markers for Pomegranate (Punica granatum L.) using an Enriched Library. Conser. Genet. Res., 2: 283-285.
14. Hasnaoui, N., Jbir, R., Mars, M., Trifi, M., Kamal-Eldin, A., Melgarejo, P. and Hernandez, F. 2011. Organic Acids, Sugars, and Anthocyanins Contents in Juices of Tunisian Pomegranate Fruits. Inter. J Food Proper., 14:741-757.
15. Holland, D. and Bar-Ya’kov, I. 2008. The pomegranate: New Interest in an Ancient Fruit. Chron. Hort., 48:12-15.
16. Holland, D., Hatib, K., and Bar-Ya’akov, I. 2009. Pomegranate: Botany, Horticulture, Breeding. Hortic. Rev., 35:127-191.
17. Kotwal, G. J. 2007. Genetic Diversity-independent Neutralization of Pandemic Viruses (e g. HIV), Potentially Pandemic (e.g. H5N1 Strain of Influenza) and Carcinogenic (e.g. HBV and HCV) Viruses and Possible Agents of Bioterrorism (Variola) by Enveloped Virus Neutralizing Compounds (EVNCs). Vaccine, 26:3055-3058.
18. Lansky, E. P. and Newman, R. A. 2007. Punica granatum (Pomegranate) and Its Potential for Prevention and Treatment of Inflammation and Cancer. J. Ethnopharm., 109:177-206.
19. Levin, G. M. 2006. Pomegranate Roads: A Soviet Botanist’s Exile from Eden. First Edition, Floreant Press, Forestville, California, USA, PP. 15-183.
20. Martínez, J. J., Melgarejo, P., Hernández, F., Salazar, D. M. and Martínez, R. 2006. Seed Characterization of Five New Pomegranate (Punica granatum L.) Varieties. Sci. Hort., 10: 241-246.
21. Melgarejo, P., Calín-Sánchez, Á., Vázquez-Araújo, L., Hernández, H., Martínez, J. J., Legua, P. and Carbonell-Barrachina, Á. A. 2011. Volatile Composition of Pomegranates from 9 Spanish Cultivars using Headspace Solid Phase Microextraction. J. Food Sci., 76: s114-s120.
22. Mena, P., García-Viguera, C., Navorro-Rico, J., Moreno, D., Bartual, J., Saura, D. and Martí, N. 2011. Phytochemical Characterization for Industrial use of Pomegranate (Punica granatum L.) Cultivars Grown in Spain. J. Sci. Food Agric., 91: 1893-1906.
23. Nemati, Z., Tehranifar, A., Farsi, M., Kakhki, A.M., Nemati, H. and Khayat, M. 2012. Evaluation of Genetic Diversity of Iranian Pomegranate Cultivars Using Fruit Morphological Characteristics and AFLP Markers. Not. Bot. Horti. Agrobo., 40: 261-268.
24. Noormohammadi, Z., Fasihee, A., Homaee-Rashidpoor, A., Sheidai, M., Baraki, S.G., Mazooji, A. and Tabatabaee-Ardakani, S.Z. 2012. Genetic Variation among Iranian Pomegranates (Punica granatum L.) using RAPD, ISSR and SSR Markers. Australian J. Crop Sci., 6(2):268-275.
25. Parvaresh, M., Talebi, M. and Sayed-Tabatabaei, B. E. 2012. Molecular Diversity and Genetic Relationship of Pomegranate (Punica granatum L.) Genotypes using Microsatellite Markers. Sci. Hort., 138: 244-252.
26. Pellegrini, N., Serafini, M., Colombi, B., Rio, D. D., Salvatore, S., Bianchi, M. and Brighenti, F. 2003. Total Antioxidant Capacity of Plant Foods, Beverages and Oils Consumed in Italy Assessed by Three Different In vitro Assays. J. Nutr., 133: 2812-2819
27. Reddy, M. K., Gupta, S. K., Jacob, M. R., Khan, S. L. and Ferreira, D. 2007. Antioxidant, Antimalarial and Antimicrobial Activities of Tannin Rich Fractions, Ellagitannins and Phenolic Acids from Punica granatum L. Planta Medica, 73: 461-467.
28. Rohlf, F. J. 1998 NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.0. Exeter Software, Setauket, NY, USA.
29. Sadeghi, N., Jannat, B., Oveisi, M. R., Hajimahmoodi, M. and Photovat, M. 2009. Antioxidant Activity of Iranian Pomegranate (Punica granatum L.) Seed Extracts. J. Agric. Sci. Tech., 11: 633-638.
30. Sarkhosh, A., Zamani, Z., Fatahi, R. and Ranjbar, H. 2009. Evaluation of Genetic Diversity among Iranian Soft-seed Pomegranate Accessions of Fruit Characteristics and RAPD Markers. Sci. Hort., 121: 313-319.
31. SAS Institute. 2005. STAT Guide for Personal Computers. Version 9.1.3. SAS Institute, Cary, North Carolina, USA.
32. Sepulveda, E., Saenz, C., Robert., P., Bartolome, B. and Gomez-Cordoves, C. 2010. Influence of the Genotype on the Anthocyanin Composition, Antioxidant Capacity and Colour of Chilean Pomegranate (Punica granatum L.) Juices. Chilean J. Agric. Res., 70: 50-57.
33. Slinkard, K. and Singleton, V. L. 1977. Total Phenol Analysis: Automation and Comparison with Manual Methods. Am. J. Enol.Vitic., 28 :49-55
34. Soriano, J. M., Zuriaga, E., Rubio, P., Llácer, G., Infante, R. and Badenes, M. L. 2011. Development and Characterization of Microsatellite Markers in Pomegranate (Punica granatum L.). Mol. Breed., 27: 119-128.
35. Stover, E. and Mercure, E.W. 2007. The Pomegranate: A New Look at the Fruit of Paradise. Hort. Sci., 42: 1088-1092.
36. Tehranifar, A., Zarei, M., Nemati, Z., Esfandiyari, B. and Vazifeshenas, M. R. 2010. Investigation of Physic-chemical Properties and Antioxidant Activity of Twenty Iranian Pomegranate (Punica granatum L.) cultivars. Sci. Hort., 126: 180-185.
37. TFFV. 2011. Turkish Fresh Fruit and Vegetables Portal. http://www.ffv.org.tr/home.aspx (accessed January 2012).
38. Tibet, H. and Onur, C. 1999. Adaptation of Pomegranate (Punica granatum L.) Cultivars in Antalya Region. Proceedings of 3rd National Horticultural Congress, 14-17 September, Ankara, Turkey, PP. 31-35.
39. Varasteh, F., Arzani, K., Zamani, Z. and Tabatabaei, S. Z. 2008. Physico-chemical Seasonal Changes of Pomegranate (Punıca granatum L.) Fruit 'Malas-e-Torsh-e-Saveh' in Iran. Acta Hort., 769: 255-258.
40. Varasteh, F., Arzani, K., Zamani, Z. and Mohseni, A., 2009. Evaluation of the Most Important Fruit Characteristics of Some Commercial Pomegranate (Punica granatum L.) Cultivars Grown in Iran. Acta Hort. 818: 103-108.
41. Varasteh, F., Arzani, K., Barzegar, M. and Zamani, Z. 2012. Changes in Anthocyanins in Arils of Chitosan-coated Pomegranate (Punica granatum L. cv. Rabbab-e-Neyriz) Fruit during Cold Storage. Food Chem., 130: 267-272
42. Zamani, Z., Sarkhosh, A., Fatahi, R. and Ebadi, A. 2007. Genetic Relationships among Pomegranate Genotypes Studied by Fruit Characteristics and RAPD Markers. J. Hort. Sci. Biotech., 82:11-18.
43. Zaouay, F. and Mars, M. 2011. Diversity among Tunisian Pomegranate (Punica granatum) Cultivars as Assessed by Pomological and Chemical Traits. Inter. J. Fruit Sci., 11: 151-166.
Journal of Agricultural Science and Technology
Volume 15, Issue 7
November and December 2013
Pages 1449-1460