Analysis of Morphological Variability in Wild Cherry (Prunus avium L.) Genetic Resources from Central Serbia

Authors
V. Rakonjac 1
E. Mratinić 2
R. Jovković 3
M. Fotirić Akšić 2
1 University of Belgrade, Faculty of Agriculture, Departman of Genetic, Plant Breeding and Seed Science, Nemanjina 6, 11080 Belgare-Zemun, Serbia.
2 University of Belgrade, Faculty of Agriculture, Departman of Fruit Science, Nemanjina 6, 11080 Belgare-Zemun, Serbia.
3 Institute for Forestry, Kneza Višeslava 3, 11000 Belgrade, Serbia.
Abstract
In thirty-three wild cherry (Prunus avium L.) accessions from Central Serbia, tree, leaf, fruit, and stone morphological characters were evaluated during three consecutive years. The goal was to detect relationships between the genotypes and to identify the most useful traits for discrimination among them. The study revealed a high variability in the set of the evaluated wild cherry accessions and considerable differences were found among them in all studied attributes. The majority of important correlations were determined among variables representing fruit and leaf size, and variables related to color. Cluster analysis distinguished wild cherry accessions into two distinct groups. In PCA, fruit and leaf traits such as leaf length and width, and fruit height, width, and weight, and skin flesh and juice color were predominant in the first two components, indicating that they were useful for the assessment of wild cherry germplasm characterization. These results indicate that these accessions must be conserved as valuable genetic resources to enrich the cherry gene pool and can be used for improving breeding efficiency of important horticultural traits worldwide.

Keywords

1. Banković, S., Medarević, M., Pantić, D., Petrović N. and Šljukić B., Obradović S. 2009. The Growing Stock of the Republic of Serbia - State and Problems. Bull. Fac. For., 100: 7-30.
2. Brown, S. K. 1988. Assessment of Fruit Firmness in Selected Sour Cherry Genotypes. HortSci., 23: 882–884.
3. Budan, S., Petre, L. and Gradinariu, G. 2009. Evaluation of Some Native Sweet Cherry Genotypes Collected ex situ into Romanian National Germplasm. Acta Hortic., 814: 157-160.
4. Demirsoy, H. and Demirsoy, L. 2004. A Study on the Relationships between Some Fruit Characteristics in Cherries. Fruits, 59: 219–223.
5. Dicenta F. and Garcia J. E. 1992. Phenotypical Correlations among Some Traits in Almond. J. Genet. Breed., 46: 241–246.
6. Frascaria, N., Santi, F. and Gouyon, P.H. 1993. Genetic Differentiation within and among Populations of Chestnut (Castanea sativa Mill.) and Wild Cherry (Prunus avium L.). Heredity, 70: 634–641.
7. Gregoriusa, H. R., Kownatzkic, D. and Höltken A. M. 2011. Spatial Patterns of Mating Relations in Wild Cherry (Prunus avium L.). Perspectives in Plant Ecology. Evol. Syst., 13: 36–44.
8. Hjalmarlsson, I. and Ortiz, R. 2000. In situ and ex situ Assessment of Morphological and Fruit Variation in Scandinavian Sweet Cherry. Sci. Hortic., 85: 37-49.
9. Holtken, A. M. and Gregorius, H. R. 2006. Detecting Local Establishment Strategies of Wild Cherry (Prunus avium L.). BMC Ecol., 6-13. http://dx.doi.org/10.1186/1472-6785-6-13
10. Iezzoni, A.F. 2008. Cherries. In: "Temperate Fruit Crop Breeding: Germplasm to Genomics", (Ed.): Hancock, J. F.. Springer Verlag, Germany, PP. 151–175.
11. Iezzoni, A. F. and Mulinix C. A. 1992. Yield Components among Sour Cherry Seedlings. J. Am. Soc. Hortic. Sci., 117: 380–383.
12. Jolivet, C., Höltken, A.M., Liesebach, H., Steiner, W. and Degen B. 2011. Spatial Genetic Structure in Wild Cherry (Prunus avium L.): I. Variation among Natural Populations of Different Density. Tree Genet. Genomes, 7: 271–283.
13. Karlidag, H., Ercisli, S., Sengul,,M. and Tosun M. 2009. Physico-chemical Diversity in Fruits of Wild-growing Sweet Cherries (Prunus avium L.). Biotechnol. Biotech. Eq., 23: 1325-1329.
14. Khadivi-Khub, A., Zamani, Z. and Fatahi, M. R. 2012. Multivariate Analysis of Prunus subgen. Cerasus Germplasm in Iran Using Morphological Variables. Genet. Resour. Crop Ev., 59: 909–926.
15. Krahl, K. H., Lansari, A. and Iezzoni, A. F. 1991. Morphological Variation within a Sour Cherry Collection. Euphytica, 52: 47–55.
16. Lacis, G., Kaufmane, E., Trajkovski, V. and Rashal, I. 2009. Morphological Variability and Genetic Diversity within Latvian and Swedish Sweet Cherry Collections. Acta Univ. Lat., 753: 19–32.
17. Lacis, G., Trajkovski, V. and Rashal I. 2010. Phenotypical Variability and Genetic Diversity within Accessions of the Swedish Sour Cherry (Prunus cerasus L.) Genetic Resources Collection. Biologija, 56: 1–8.
18. Li, M. M., Cai, Y. L., Qian, Z. Q. and Zhao, G. F. 2009. Genetic Diversity and Differentiation in Chinese Sour Cherry Prunus pseudocerasus Lindl., and Its Implications for Conservation. Genet. Resour. Crop Ev., 56: 455–464.
19. Mariette, S., Lefranc, M., Legrand, P., Taneyhill, D., Frascaria-Lacoste, N. and Machon, N., 1997. Genetic Variability in Wild Cherry Populations in France. TAQ, 94: 904-908.
20. Martínez-Calvo, J., Gisbert, A. D., Alamar, M. C., Hernandorena, R., Romero, C., Llacer, G. and Badenes, M. L. 2008. Study of a Germplasm Collection of Loquat (Eriobotrya japonica Lindl.) by Multivariate Analysis. Genet. Resour. Crop Ev., 55: 695–703.
21. Moreno, J. and Trujillo, I., 2005. Genetic Characterization and Relatedness among Cherry Cultivars in a Germplasm Bank by Randomly Amplified Polymorphic DNA Analysis. Agr. Conspec. Sci., 70: 105-111.
22. Mratinić E., Popovski, B., Miloševic, T. and Popovska, M. 2011. Analysis of Morphological and Pomological Characteristics of Apricot Germplasm in FYR Macedonia. J. Agr. Sci. Tech., 13: 1121-1134.
23. Mattos, A. L., Amorim, P. E., Amorim, O. B. V., Cohen, O. K., Lodo, S. A. C. and Silva, S. O. 2010. Agronomical and Molecular Characterization of Banana Germplasm. Pesq. Agrop. Bras., 45: 146-154.
24. Pérez-Sánchez, R., Gómez-Sánchez, M. A. and Morales-Corts, R. 2008. Agromorphological Characterization of Traditional Spanish Sweet Cherry (Prunus avium L.), Sour Cherry (Prunus cerasus L.) and Duke Cherry (Prunus x gondouinii Rehd.) Cultivars. Span. J. Agric. Res., 6: 42-55.
25. Pérez-Sánchez, R., Gómez-Sánchez, M. A. and Morales-Corts, M. R. 2010. Description and Quality Evaluation of Sweet Cherries Cultured in Spain. J. Food Quality, 33: 490–506.
26. Radičević, S., Cerović, R., Mitrović, O. and Glišić, I. 2008. Pomological Characteristics and Biochemical Fruit Composition of Some Canadian Sweet Cherry Cultivars. Acta Hortic., 795: 283-286.
27. Rakonjac, V., Fotirić Akšić, M., Nikolić, D., Milatović, D. and Čolić, S. 2010. Morphological Characterization of ‘Oblačinska’ Sour Cherry by Multivariate Analysis. Sci. Hortic., 125: 679–684.
28. Reim, S., Proft, A., Heinz, S. and Hofer, M. 2012. Diversity of the European Indigenous Wild Apple Malus sylvestris (L.) Mill. in the East Ore Mountains (Osterzgebirge), Germany: I. Morphological Characterization. Genet. Resour. Crop Ev., 59: 1101–1114.
29. Ruisa, S., 1998. Genetic Resources of Latvian Sweet Cherries and Their Use. Acta Hortic., 468: 153-160.
30. Schmidt, H., Vittrup-Christensen, J., Watkins, R. and Smith, R.A. 1985. Cherry Descriptors. CEC Secretariat, Brussels, IBPGR Secretariat, Rome, PP. 1-33
31. Stojecová, R. and Kupka, I., 2009. Growth of Wild Cherry (Prunus avium L.) in a Mixture with Other Species in a Demonstration Forest. J. For. Sci., 55: 264–269.
32. Tehrani, G. and Brown, S. K. 1992. Pollen-incompatibility and Self Fertility in Sweet Cherry. Plant Breed. Rev., 9: 368–370.
33. Theiler-Hedricth, R. 1990. Relationships between Fruit Weight and Diameter in Cherries. SZOW, 126: 590–598.
34. UPOV. 2002. Guidelines for the Conduct of Tests for Distinctness, Uniformity and Stability. European Plum (Prunus domestica L.). TG/41/5, Genova, Italy, PP. 1–31.
35. Usenik, V., Fabčič, J. and Štampar, F. 2008. Sugars, Organic Acids, Phenolic Composition and Antioxidant Activity of Sweet Cherry (Prunus avium L.). Food Chem., 107: 185-192.
36. Vaughan, S. P., Cottrell, J.E., Moodley, D. J., Connolly, T. and Russell K. 2007. Clonal Structure and Recruitment in British Wild Cherry (Prunus avium L.). Forest Ecol. Manag., 242: 419–430.
37. Webster, A. D. 1996. The Taxonomic Classification of Sweet and Sour Cherries and a Brief History of Their Cultivation. In: "Cherries: Crop Physiology, Production and Uses", (Eds.): Webster A. D. and Looney N. E.. CABI, Wallingford CT, PP. 3–24.
38. Whiting, M. D., Lang, G. and Ophardt, D. 2005. Rootstock and Training System Affect Sweet Cherry Growth, Yield and Fruit Quality. HortSci., 40: 582-586.
39. Zeinali, H., Tabaei-Aghdaei, S. R. and Arzani, A. 2009. A Study of Morphological Variations and Their Relationship with Flower Yield and Yield Components in Rosa damascena. J. Agric. Sci. Technol., 11: 439-448.
Journal of Agricultural Science and Technology
Volume 16, Issue 1
January and February 2014
Pages 151-162