Improvement of Sperm Quality Indices of Benni Fish (Barbus sharpeyi) by Application of LHRHA2 and Metoclopramide

Authors
1 Faculty of Marine Sciences, Tarbiat Modares University, P. O. BOX: 46414, Noor, Islamic Republic of Iran.
2 IFRO, South Iran Aquaculture Research Center, P. O. BOX: 866/61645, Ahvaz, Islamic Republic of Iran.
Abstract
The main objective of this study was assessment of hormonal treatments effects on sperm quality improvement in Barbus sharpeyi. Results showed differences in effects of treatments with LHRHa2, LHRHa2 combined with a dopamine antagonist (MET), and CPE (Carp Pituitary Extract) on parameters of sperm volume, motility, spermatocrit, biochemical indices of seminal plasma and sperm efficiency on artificial propagation, incubation survival, and deformities. Biochemical analysis of seminal plasma showed that the majority of parameters were affected by different hormonal treatments. In conclusion, the highest sperm quality of Benni fish could be achieved by application of LHRHA2 (10 µg kg-1+MET), LHRHA2 (5 µg kg-1+MET) and LHRHA2 (5 µg kg-1) at 8, 12, and 16 hours PHS, respectively.

Keywords


1. Agarwal, N. K. and Raghuvanshi, S. K. 2009. Spermatocrit and Sperm Density in Snowtrout (Schizothorax richardsonii): Correlation and Variation during the Breeding Season. Aquacult., 291: 61-64.
2. Al Mukhtar, M. A., Saleh, J. H., Jaber, A. A. and Hatam, A. 2009. Artificial Propagation and Fingerlings Production of Barbus sharpeyi (Gunther 1874) in Basrah during the Spring of 2006. Iraqi J. Agric., 14: 87-193.
3. Alavi, S. M. H., Cosson, J., Karami, M., Mojazi Amiri, B. and Akhoundzadeh, M. A. 2004. Spermatozoa motility in the Persian Sturgeon, Acipenser persicus: effects of pH, dilution rate, ions and osmolality. Reproduction, 128: 819-828.
4. Alavi, S. M. H., Jorfi, E., Hatef, A. and Mortazavi, S. A. S. 2010. Sperm Motility and Seminal Plasma Ccharacteristics in Barbus sharpeyi (Gu¨nther, 1874). Aquacult. Res., 41: 688-694.
5. Alavi, S. M. H., Rodina, M., Viveiros, A. T. M., Cosson, J., Gela, D., Boryshpolets, S.and Linhart, O. 2009. Effects of Osmolality on Sperm Morphology, Motility and Flagellar Wave Parameters in Northern Pike (Esox lucius L.). Theriogenol., 72: 32-43.
6. Albert, J. B. 2008. An Overview of 30 Years of International Research in Some Selected Fields of the Reproductive Physiology of Fish. Cybium. Suppl., 32: 7-13.
7. Arabaci, M., Cagirgan, H. and Sari, M. 2001. Induction of Spawning in Common Carp (Cyprinus carpio) using LHRHa ([D-Ser (tBu)6, Pro9-NEt]-LHRH) combined with Haloperidol: Effects of Different Treatment Time and Determination of Latency Period Dependence on Temperature. Turkish J. Fish. Aquat. Sci., 1: 1-5.
8. Aramli, M. S., Nazari R. M., Kalbassi M. R. and Aramli S. 2013. Effects of Short-term Storage on the Motility, Oxidative Stress, and ATP Content of Persian Sturgeon (Acipenser persicus) Sperm. Anim. Reprod. Sci. 143(1-4):112-7.
9. Aramli, M. S., Kalbassi M. R. and Nazari R. M. 2013. Sex Steroid Levels of Persian Sturgeon, Acipenser persicus, Borodin, 1897, Males in Negative and Positive Responding to LH-RH-analogue. J. Appl. Ichthyol. DOI: 10.1111/jai.12347
10. Aramli, M. S., Kalbassi M. R. and Nazari R. M. 2013. Comparative Study of Sex Steroid Levels of Persian Sturgeon, Acipenser persicus Males in Responding Negative and Positive to LHRH-A2 Hormone. J. Aquac. Res. Development. 4:177-182
11. Aramli, M. S., Kalbassi M. R. and Nazari R. M. 2013. Study of Sperm Concentration, Seminal Plasma Composition and their Physiological Correlation in the Persian Sturgeon, Acipenser persicus. Reprod. Domest. Anim. DOI: 10.1111/rda.12207
12. Bonnet, E., Fostier, A. and Bobe, J. 2007. Characterization of Rainbow Trout Egg Quality: A Case Study Using Four Different Breeding Protocols, with Emphasis on the Incidence of Embryonic Malformations. Theriogenol., 67: 786-794.
13. Bozkurt, Y., Ogrctmen, F., Secer, F. S. and Ercin, U. 2009. Relationship between Seminal Plasma Composition and Spermatological Parameters in Scaly Carp (Cyprinus carpio). J. Anim. Vet. Adv., 8: 2745-2749.
14. Bozkurt, Y., Ogretmen, F., Kokcu, O. and Ercin, U. 2011. Relationships between Seminal Plasma Composition and Sperm Quality Parameters of the Salmo trutta macrostigma (Dumeril, 1858) Semen: With Emphasis on Sperm Motility. Czech J. Anim. Sci., 56: 355–364.
15. Bozkurt, Y., Secer, S., Bukan, N., Akcay, E. and Tekin, N. 2006. Relationship between Body Condition, Sperm Quality Parameters and Fertilization Success in Rainbow Trout (Salmo trutta fario) Sperm. Pak. J. Biol. Sci., 9: 940-944.
16. Caille, N., Rodina, M., Kocour, M., Gela, D., Hans, F. M., and Linhart, O. 2006. Quantity, Motility and Fertility of Tench Tinca tinca (L.) Sperm in Relation to LHRH Analogue and Carp Pituitary Treatments. Aquacult. Int., 14: 75–87.
17. Cejko, B. I., Kowalski, R. K., Kucharczyk, D., Targońska, K., Krejszeff, S., Żarski, D. and Glogowski, J. 2010. Influence of the Length of Time after Hormonal Stimulation on Selected Parameters of Milt of Ide Leuciscus idus L. Aquacult. Res., 41: 804–813.
18. Cejko, B. I., Kowalski, R. K., Zarski, D., Targonska, K., Chwaluczyk, R., Kucharczyk, D., Glogowski, J. 2012. The Influence of the Length of Time after Hormonal Treatment with [(d-Ala6, Pro9 NEt)-mGnRH+Metoclopramide] i.e. Ovopel on Barbel Barbus barbus (L.) Milt Quality and Quantity Indicators. J. Appl. Ichthyol., 28: 249–253.
19. Cejko, B. I., Kucharczyk, D., Targonska, K., Kubiak, D., Sarosiek, B. and Glogowski, J. 2008. Quality Parameters and Selected Biochemical Markers of ASP, Aspius Aspius (L.), Semen Obtained after Hormonal Stimulation with Ovaprim or Ovopel. Arch. Pol. Fish., 16: 179-188.
20. Cejko, B., Kowalski, R. K., Kucharczyk, D., Zarski, D., Targonska, K. and Glogowski, J. 2011. Effect of Time after Hormonal Stimulation on Semen Quality Indicators of Common Carp, Cyprinus carpio (Actinopterygii: Cypriniformes: Cyprinidae). ACTA Ichthyol. Pisc., 41: 75-80.
21. Ciereszko, A., Dabrowski, K. 1994. Relationship between Biochemical Constituents of Fish Semen and Fertility: The Effect of Short-term Storage. Fish Physiol. Biochem., 12: 357-367.
22. Golpour, A. and Imanpoor, M. R. 2010. Relationships between Compositions of Seminal Plasma and Blood Plasma Parameters in Caspian Roach (Rutilus rutilus caspicus) during the Reproductive Season. Res. J. Fish. Hydrobiol., 5:105-110.
23. Hajirezaee, S., Mojazi Amiri, B. and Mirvaghefi, A. 2010. Fish Milt Quality and Major Factors Influencing the Milt Quality Parameters: A Review. Afr. J. Biotechnol., 9: 9148-9154.
24. Hashim, A. L., Hussain, A. N. A., Wazir, A. R., Hamid, W. A. and Balasem, A. 2006. The New Eden Master Plan for Integrated Water Resource Management in the Marshland Areas.–Agriculture: Overview of Present Conditions and Current Use of the Water in the Marshland Area. New Eden Group, Italy-Iraq, 4(1): 256.
25. Horváth, A., Miskolczi, E., Mihalffy, S., Osz, K., Szabo, K. and Urbanyi, B. 2007. Cryopreservation of Common Carp (Cyprinus carpio) Sperm in 1.2 and 5 ml Straws and Occurrence of Haploids among Larvae Produced with Cryopreserved Sperm. Cryobiol., 54: 251–257.
26. Hwang, P. C. and Idler, D. R. 1969. A Study of Major Cations, Osmotic Pressure, and pH in Seminal Components of Atlantic Salmon. J. Fish. Res. Bd . Can., 26: 413-419.
27. Jezierska, B., Lugowska, K., Witeska, M. and Sarnowski, P. 2000. Malformation of Newly Hatched Common Carp Larvae. Electronic Journal of Polish Agricultural Universities. Available via http://www.ejpau.media.pl/volume3/issue2/fisheries/art-01.html.
28. Kahkesh, F. B., Yooneszadeh Feshalami, M., Amiri, F. and Nickpey, M. 2010. Effect of Ovaprim, Ovatide, HCG, LHRH-A2, LHRHA2+CPE and Carp Pituitary in Benni (Barbus sharpeyi) Artificial Breed. Glob. Vet., 5: 209-214.
29. Kalbassi M. R. and lorestani R. 2013. Analysis of Saline Activator Solution Effects on Sperm Quality Indices of Barbus sharpeyi by Image J Software. Iran. J. Fish. Sci. 12: 357-377.
30. Krejai, R. and Palikova, M. 2006. Potassium Dichromate as a Reference Substance for Embryonic Tests of Toxicity in the Common Carp (Cyprinus carpio L.). ACTA Vet. Brno., 75: 259–263.
31. Lahnsteiner, F., Berger, B., Weismann, T. and Patzner, R. 1995. Fine Structure and Motility of Spermatozoa and Composition of the Seminal Plasma in the Perch. J. Fish. Biol., 47: 492-508.
32. Lahnsteiner, F., Berger, B., Weismann, T., Patzner, R. A. 1996. Motility of Spermatozoa of Alburnus alburnus (Cyprinidae) and Its Relationship to Seminal Plasma Composition and Sperm Metabolism. Fish Physiol. Biochem., 15:167-179.
33. Li, W. S., Lin, H. R. and Wong, A. O. 2002. Effects of Gonadotropin-Releasing Hormone on Growth Hormone Secretion and Gene Expression in Common Carp Pituitary. Comp. Biochem. Physiol., 132: 335–341.
34. Lin, F., Ciereszko, A. and Dabrowski, K. 1996. Sperm Production and Cryopreservation in Muskellunge after Carp Pituitary Extract and Human Chorionic Gonadotropin Injection. Progressive Fish Cult., 58: 32-37.
35. Linhart, O., Alavi, S. M. H., Rodina, M., Gela, D. and Cosson, J. 2008. Comparison of Sperm Velocity, Motility and Fertilizing Ability between Firstly and Secondly Activated Spermatozoa of Common Carp (Cyprinus carpio). J. Appl. Ichthyol., 24: 386-396.
36. Mabudi, H., Jamili, S., Majd, N. E., Vosoughi, G., Fatemi, M. R. and Rashed, S. 2011. The Effects of Ghrelin on Ovary Histology in Barbus sharpeyi. J. Anim. Physiol. Anim. Nutr., 95: 599-602.
37. Mis, J., Bieniarz, K., Epler, P., Sokolowska-Mikolajczyk, M. and Chyb, J. 1995. Incubation of Fertilized Common Carp (Cyprinus carpio L.) Eggs in Different Concentrations of Copper. Pol. Arch. Hydrobiol., 42: 269-276.
38. Morisawa, M. 1985. Initiation Mechanism of Sperm Motility at Spawning in Teleosts. Zool. Sci., 2: 605-615.
39. Morisawa, M., Suzuki, K. and Morisawa, S. 1983. Effects of Potassium and Osmolality on Spermatozoan Motility of Salmonid Fishes. J. Exp. Biol., 107: 105-113.
40. Mylonas, C. C., Fostier, A. and Zanuy, S. 2010. Broodstock Management and Hormonal Manipulations of Fish Reproduction. Gen. Comp. Endocrinol., 165: 516-534.
41. Ottesen, O. H. and Babiak, I. 2007. Parental Effects on Fertilization and Hatching Success and Development of Atlantic Halibut (Hippoglossus hippoglossus L.) Embryos and Larvae. Theriogenol., 68:1219–1227.
42. Pyka, J., Bartel, R., Szczerbowski, J. A. and Epler, P. 2001. Reproduction of Gattan (Barbus xanthopterus Heckel), Shabbout (Barbus grypus Heckel) and Bunni (Barbus sharpeyi Gunther) and Rearing Stocking Material of Theses Species. Arch. Pol. Fish., 9: 235-246.
43. Tan-Fermin, J. D., Miura, T., Adachi, S. and Yamauchi, K. 1999. Seminal Plasma Composition, Sperm Motility and Milt Dilution in the Asian Catfish Clarias macrocephalus (Gunther). Aquacult., 171: 323- 338.
44. Verma, D. K, Routray, P., Dash, C., Dasgupta S., Jena, J. K. 2009. Physical and Biochemical Characteristics of Semen and Ultrastructure of Spermatozoa in Six Carp Species. Turk. J. Fish. Aquat. Sci., 9: 67-76.
45. Von Westernhagen, H., Hoar, W. S. and Randall, D. J. 1988. Sublethal Effects of Pollutants on Fish Eggs and Larvae. Fish Physiol., 11: 253-346.
46. Wilson-Leedy, J. G., Kanuga, M. K. and Ingermann, R. L. 2009. Influence of Osmolality and Ions on the Activation and Characteristics of Zebrafish Sperm Motility. Theriogenol., 71:1054–1062.