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Effect of entomopathogenic nematode and fungi on mortality and development of Spodoptera frugiperda (J.E. Smith) larvae
Adel A. Al-Ayat1 , Ahmed M. Gharib1 , Mahmoud M.M. Hassuba1 , Ayman A.M. Atta2 , Hassan A. Mesbah3 , Hassan Gad * 4
1- Plant Protection Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
2- plant Protection Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
3- Department of Agricultural Botany (Genetics), Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
4- Plant Protection Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt , hassangad1985@azhar.edu.eg
Abstract:   (352 Views)
The present study was conducted to evaluate the efficacy of entomopathogenic nematode, Steinernema carpocapsae Weiser and three entomopathogenic fungi (Metarhizium anisopliae (Metschn.) Sorokin, Trichoderma harzianum Rifai, and T. viride Pers.) against Spodoptera frugiperda (J.E. Smith) second and fourth larval instars. The results showed that S. carpocapsae caused a pronounced mortality to second and fourth larval instars of S. frugiperda using a leaf dipping method at 4 days post inoculation (dpi) with LC50 values 52.03 and 4.11 infective juveniles (IJs)/ml, respectively.  On the other hand, the three tested entomopathogenic fungi caused a strong toxicity on larval instars of S. frugiperda. The fungus, T. harzianum, displayed the highest insecticidal activity on second larval instar (LC50=1.1×107 spores/ml) and M. anisopliae on fourth larval instar (LC50=1.5×107 spores/ml) after 10 dpi. Our results showed that S. carpocapsae completely inhibited pupation and adult emergence from treated larvae at 250 IJs/ml. The lethal effect of entomopathogenic nematode and fungi against S. frugiperda larval instars indicates that these biological control agents could be useful candidates in integrated pest management programs for this invasive insect.
 
Keywords: Fall armyworm, Steinernema carpocapsae, Metarhizium anisopliae, Trichoderma harzianum
Full-Text [PDF 362 kb]   (221 Downloads)    
Article Type: Original Research | Subject: Ecology and Biological Control
Received: 2023/09/17 | Accepted: 2024/01/11 | Published: 2024/03/31
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