First Record of Three Known Species of the Family Tylenchidae Örley, 1880 (Nematoda: Tylenchina) from Iran with New Morphological and Molecular Data

Authors
Y. Panahandeh
E. Pourjam
F. Aliramaji
M. R. Atighi
M. Pedram
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
Abstract
Three species belonging to three genera of the family Tylenchidae, namely, Cephalenchus leptus, Eutylenchus excretorius, and Lelenchus leptosoma were discovered as first reports for Iran's nematode fauna. They were characterized by morphological, morphometric and molecular phylogenetic studies. The two latter genera are new for Iran. Iranian population of Cephalenchus leptus is characterized by lateral field with six lines in females, stylet length of 16-20 µm and tail length of 153-290 µm with pointed tip. The recovered population of Eutylenchus excretorius is characterized by 848-1,038 µm long females’ body, stylet length of 20.0-21.5 µm, having advulval flaps and absence of male. The Iranian population of Lelenchus leptosoma is defined by its narrow slender body, flattened lip region, sinuous amphidial opening and absence of lateral field. The phylogenetic relationships of the three recovered species were studied using the partial sequences of 28S rDNA D2/D3 segment and revealed the genera Cephalenchus and Eutylenchus forming a monophyletic clade, while, Lelenchus was placed inside the clade of currently sequenced species of Malenchus in Bayesian tree.

Keywords

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Journal of Agricultural Science and Technology
Volume 17, Issue 7
November and December 2015
Pages 1903-1918