Volume 23, Issue 6 (2021)                   JAST 2021, 23(6): 1355-1366 | Back to browse issues page

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Entezari A, Mehrvar M, Zakiaghl M. First Nearly Complete Genome Sequence of Onion Yellow Dwarf Virus Infecting Garlic in Iran. JAST. 2021; 23 (6) :1355-1366
URL: http://jast.modares.ac.ir/article-23-46720-en.html
1- Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Islamic Republic of Iran.
2- Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Islamic Republic of Iran. , mehrvar@um.ac.ir
Abstract:   (232 Views)
For the first time, the nearly complete genome sequence of onion yellow dwarf virus (OYDV) was found in Iran from garlic (Allium sativum L.) by deep RNA sequencing. Complete coding sequence of the Iranian isolate of OYDV (MN528769) consists of 10,212 nucleotides (nt), encodes a polyprotein with 3,403 amino acids (aa). Pairwise sequence comparisons showed that IR-Kh2 shares 74.84-97.39% identity at the nt level and 75.67-98% identity at the amino acids level, respectively with other OYDV isolates deposited in the GenBank previously. According to the phylogenetic analysis, the OYDV isolates were divided into two main groups based on the coding sequence of genome and the Iranian OYDV isolate cluster together with the Australian (MS/SW1), Spanish (SG1), Chinese (G78 and G37-2), and Indian (RR1) isolates. Furthermore, a genetic recombination analysis was also performed, in which a putative recombination event was detected in the nuclear inclusion body b (NIb) gene.
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Article Type: Original Research | Subject: Plant Protection/Plant virology
Received: 2020/10/10 | Accepted: 2021/06/29 | Published: 2021/10/27

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