Volume 22, Issue 3 (2020)                   JAST 2020, 22(3): 863-874 | Back to browse issues page

‎ 20.1001.1.16807073.2020.22.3.17.7

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Singh S, Ram S, Narwal S. Identification of Genome Specific Sequence Motifs in α-Gliadins and Wheat Accessions with Less Celiac Disease Epitopes. JAST 2020; 22 (3) :863-874
URL: http://jast.modares.ac.ir/article-23-27824-en.html
Identification of Genome Specific Sequence Motifs in α-Gliadins and Wheat Accessions with Less Celiac Disease Epitopes
S. Singh * 1, S. Ram2 , S. Narwal2
1- Lovely Professional University, Phagwara-144411, Punjab, India. , sanjaydbtster@gmail.com
2- Indian Institute of Wheat & Barley Research (IIWBR), Karnal-132001 (Haryana), India.
Abstract:   (2304 Views)
Among gliadins, α-gliadins are important active proteins in triggering celiac disease in human beings owing to the presence of toxic epitopes. A set of 177 α-gliadin gene sequences and the corresponding proteins were analyzed. Twenty accessions of hexaploids including 1, 14, and 5, respectively representing A, B, and D, with no intact CD-epitopes in α-gliadins, were identified. Twenty-two and 13 conserved motifs in non-repetitive domains NR1 and NR2, respectively, of α-gliadins differentiated all the amino acid sequences encoded by A genome of both diploids and hexaploids. Most of the amino acid sequences encoded by D genome (70 of 75 in hexaploids and 13 of 16 in diploids) could be identified by 22 amino acid motif. Large variations and lesser number of intact CD-epitopes was observed for α-gliadins belonging to B genome. As compared to diploids, repeat length of polyglutamine repetitive domain QII of B genome was lower in hexaploids indicating loss of Q residues during evolution of hexaploid wheat. The information can be used in assigning any α-gliadin sequences onto A, B, and D genomes and identifying wheat accessions with lesser CD-epitopes. The result presented here will be useful for the wheat improvement programs aiming for the management of celiac disease in human beings.
 
Keywords: Conserved motifs, Genome, Triticum aestivum, Wheat improvement.
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Article Type: Original Research | Subject: Bioinformatics
Received: 2018/12/4 | Accepted: 2019/05/25 | Published: 2020/04/22
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