Volume 6, Issue 3 (2004)                   JAST 2004, 6(3): 129-137 | Back to browse issues page

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Mapping the Gene for Aroma in Rice (Oryza sativa L.) by Bulk Segregant Analysis via RAPD Markers. JAST. 6 (3) :129-137
URL: http://jast.modares.ac.ir/article-23-2196-en.html
Abstract:   (5678 Views)
The sensory qualities of food such as aroma, taste, and texture are the most important criteria for distinguishing Basmati type rices from non-Basmati types. To map the gene(s) controlling aroma, bulked segregant analysis (BSA) using Random Amplified Polymorphic DNA (RAPD) markers was applied in an F2/F3 population of Basmati 370 (aromatic) and IR36 (non-aromatic). DNA samples from homozygous aromatic and homozygous non-aromatic plants identified on the basis of progeny tests were bulked and used for BSA. A total of 550 random primers were used and the primers, AG8 and AN1, produced polymorphism between aromatic and non-aromatic types. Association of AG8-AR, AN1-AR1, and AN1-AR2 with a gene for aroma was determined by surveying F2 individuals. The three RAPD markers AG8-AR, AN1-AR1, and AN1-AR2 were found to be linked to the gene for aroma with a distance of 6.9, 8.9 and 16.4 cM, respectively. Southern analysis with AG8-AR as a probe with 125 F2 individuals confirmed linkage between AG8-AR and the gene for aroma. AG8-AR was mapped on chromosome 8 flanked by two tightly linked markers, RZ617 and RG978, at 2.1 and 1.7 cM distances, respectively, indicating that this gene in Basmati 370 is located on chromosome 8.
Full-Text [PDF 286 kb]   (9273 Downloads)    
Subject: Plant Breeding
Received: 2010/02/8 | Accepted: 2010/02/8 | Published: 2010/02/8

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