Roya Kazemi1 
, Mehdi Saadati1 , Ali Mokhtasi2 , Majid Pedram1 , Mohammad Reza Atighi1 , Masoud Shams-bakhsh * 
3
, Mehdi Saadati1 , Ali Mokhtasi2 , Majid Pedram1 , Mohammad Reza Atighi1 , Masoud Shams-bakhsh * 3
1- Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2- Department of Agronomy, Faculty of Agriculture, Tarbiat Modares University, PO Box 14115-336, Tehran, Iran
3- Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran ,shamsbakhsh@gmail.com
2- Department of Agronomy, Faculty of Agriculture, Tarbiat Modares University, PO Box 14115-336, Tehran, Iran
3- Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran ,
Abstract: (178 Views)
The beet cyst nematode (BCN) (Heterodera schachtii), and beet curly top virus-severe (BCTV-Svr) (Curtovirus betae) are two important pathogens of sugar beet fields worldwide. Therefore, the reaction of 14 genotypes was separately assessed, using Jolgeh and Sanetta cultivars as susceptible and resistant controls, respectively, in completely randomized design experiments for BCN and BCTV-Svr. Reactions were based on the cyst and egg counts and symptoms severity index. Experiments were performed in the greenhouse of Tarbiat Modares University, Tehran, Iran, and were repeated twice independently. Based on the results of initial experiments, the S1-960090, S1-940324, S1-960294, and S1-960284 genotypes resistant to the BCN were selected for further investigation. Furthermore, the reaction of the four selected genotypes to BCN, BCTV-Svr, and the combination (mixture) of the two pathogens was evaluated by analyzing their growth, physiological, and biochemical characteristics, and virus accumulation. Resistant genotypes showed higher levels of defense-related enzymes such as catalase, guaiacol peroxidase, and polyphenol oxidase, whereas susceptible genotypes exhibited significant reductions in photosynthesis, greenness, and chlorophyll a, b, and carotenoid content compared to non-inoculated and resistant plants. This is the first study conducted to search for dual-resistance sources against two devastating pathogens that frequently occur in the sugar beet-growing regions of Iran. Based on the results of this experiment, genotypes S1-960090 and S1-940324 were identified as resistant to both pathogens and are recommended for breeding purposes.
Article Type: Original Research |
Subject:
Ecology and Biological Control
Received: 2024/04/26 | Accepted: 2024/01/1
Received: 2024/04/26 | Accepted: 2024/01/1
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