1- University of Tabriz , a.hatami0818@gmail.com
2- University of Tabriz/ Ege University
3- University of Tabriz
2- University of Tabriz/ Ege University
3- University of Tabriz
Abstract: (379 Views)
Entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae are important and effective biocontrol agents against arthropod pests. Compared to chemical insecticides, insect pests do not easily develop resistance against these fungi. In this study, the mortality and phenol-oxidase activity of the Helicoverpa armigera 3rd instar larvae were evaluated after exposure to the B. bassiana and M. anisopliae. The LC50 values for B. bassiana and M. anisopliae were 0.795 ×106, and 5.972 ×107 spore ml-1, respectively. LC30 and LC10 of either entomopathogenic fungi were injected into body of larves, then, 24 and 48 hours after injection, their hemolymph was extracted. After 24 h the highest and lowest phenol-oxidase activity was observed in LC30 of M. anisopliae, and LC10 of B. bassiana, respectively. After 48 h of infection, phenol-oxidase activity increased in all treatments. At the LC30 of M. anisopliae, the highest phenol-oxidase activity was recorded, and other treatments also showed a significant difference compared to the control. Five types of hemocytes including prohemocytes, plasmatocytes, granulocytes, onocytoids, and spherulocytes were identified in the hemolymph of larvae. The highest total hemocytes count (THC) was recorded in LC30 M. anisopliae at 9 h after initial infection. Our results showed that both fungi have the ability to affect phenol-oxidase enzyme activity. These microbial insecticides exhibited high potential for controlling the pest.
Article Type: Original Research |
Subject:
Toxicology and Physiology
Received: 2023/10/28 | Accepted: 2024/01/1
Received: 2023/10/28 | Accepted: 2024/01/1
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