Rosa Aghaei Meybodi1 
, Mahmood Nafisi1 , Ahmad Ghasemi2 , Reza Davoodi1 , Vahid Morshedi * 3, Ebrahim Sotoude1 , Ghasem Rashidian4
, Mahmood Nafisi1 , Ahmad Ghasemi2 , Reza Davoodi1 , Vahid Morshedi * 3, Ebrahim Sotoude1 , Ghasem Rashidian4
1- Department of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, Islamic Republic of Iran.
2- Persian Gulf Research Institute, Persian Gulf University, Bushehr, Islamic Republic of Iran.
3- Persian Gulf Research Institute, Persian Gulf University, Bushehr, Islamic Republic of Iran. ,v.morshedi@gmail.com
4- Jihoceská univerzita v Ceských Budejovicích, Fakulta rybárství a ochrany vod, Jihoceské výzkumné centrum akvakultury a biodiverzity hydrocenóz, Ústav akvakultury a ochrany vod, Na Sádkách 1780, 370 05 Ceské Budejovice.
2- Persian Gulf Research Institute, Persian Gulf University, Bushehr, Islamic Republic of Iran.
3- Persian Gulf Research Institute, Persian Gulf University, Bushehr, Islamic Republic of Iran. ,
4- Jihoceská univerzita v Ceských Budejovicích, Fakulta rybárství a ochrany vod, Jihoceské výzkumné centrum akvakultury a biodiverzity hydrocenóz, Ústav akvakultury a ochrany vod, Na Sádkách 1780, 370 05 Ceské Budejovice.
Abstract: (510 Views)
This study evaluated the interactive impacts of water temperature and feeding rate on digestive enzymes, intestine histology, growth and stress-related genes, and cultivable intestinal microbiota of Asian seabass (Lates calcarifer). For this purpose, 180 fish (85.0±3.0 g) were reared at three different temperatures (20, 27, and 33°C) and two feeding rates (apparent satiation and 2.5% of biomass) with three replications for 6 weeks. The results revealed no significant differences among different treatments regarding the activity of digestive enzymes (P˃ 0.05) of fish reared under different temperatures and feeding rates. The length, width, and thickness of intestinal villi were unaffected by different temperatures and feeding rates (P˃ 0.05). In addition, no variations were found in the total aerobic bacterial count of fish gut from different experimental groups (P˃ 0.05). At the molecular level, IGF-I and HSP70 coding genes were found to be highly expressed in experimental treatments (P< 0.05). To conclude, the present study showed that temperatures between 27 to 33°C were more optimal for Asian seabass, and the different temperatures and feeding rates did not affect the digestive enzymes, intestine histology, and gut microbiota of juvenile Asian seabass after 6 weeks.
Keywords: Asian seabass, Digestive enzymes, Feeding rate, Gene expression, Gut microbiota, Temperature.
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