Lucas Wachholz1 
, Cleison de Souza1 , Clauber Polese1 , Jomara Broch1 , André Sanches de Avila1 , Vaneila Daniele Lenhart Savaris1 , Nilton Rohloff Junior1 , Tânia Luiza Köhler1 , Jansller Luiz Genova2 
, Ricardo Vianna Nunes * 3
, Cleison de Souza1 , Clauber Polese1 , Jomara Broch1 , André Sanches de Avila1 , Vaneila Daniele Lenhart Savaris1 , Nilton Rohloff Junior1 , Tânia Luiza Köhler1 , Jansller Luiz Genova2 , Ricardo Vianna Nunes * 3
1- Animal Science Department, Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, PR 85960-000, Brazil
2- Animal Science Department, Universidade Federal de Viçosa, MG 85960-000, Brazil
3- Animal Science Department, Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, PR 85960-000, Brazil ,nunesrv@hotmail.com
2- Animal Science Department, Universidade Federal de Viçosa, MG 85960-000, Brazil
3- Animal Science Department, Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, PR 85960-000, Brazil ,
Abstract: (329 Views)
Sample storage and fasting times leads to some changes of blood metabolite in broilers. Therefore, a study was conducted with the aim to assess the influence of storage and fasting times in serum and plasma fractions on glucose, total cholesterol, triacylglycerols, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma-glutamyl transferase (GGT) concentrations in broilers. A total of 70 male broiler chickens fasted at 7 times (0, 2, 4, 6, 8, 10, and 12 h) to collect blood fractions (serum and plasma) stored at -20 °C for 0, 30, and 60 days. Glucose and GGT were affected by fasting times×blood fraction. Serum glucose concentration decreased linearly (≅ 2.48 mg dL-1), whereas total cholesterol and plasma GGT increased linearly (≅ 0.92 mg dL-1 and ≅ 0.19 IU L-1, respectively) with fasting time. There was a quadratic effect on plasma glucose and serum GGT (maximum at 3.95 h and minimum at 5.22 h of fasting, respectively), and triacylglycerol (minimum at 8.75 h of fasting) and ALT concentrations (maximum at 8.45 h of fasting). Glucose, total cholesterol, AST, ALT, GGT concentrations were higher in serum, while triacylglycerols were higher in plasma. Glucose concentration had the lowest values at 30 days, while ALT was higher on day 0. However, GGT concentrations were lower on days 0 and 30. Samples of plasma for glucose, ALT, and GGT stored at -20 °C for long periods should be avoided. In addition, serum samples and 6 h fasting are recommended for the assessment of blood biochemical metabolites in broilers.
Article Type: Original Research |
Subject:
Insect Physiology
Received: 2023/05/20 | Accepted: 2024/01/1
Received: 2023/05/20 | Accepted: 2024/01/1
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