1- PhD in Agricultural Biotechnology, University campus 2, University of Guilan and Rice Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.
2- Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran ,r.shirzadian@gmail.com
2- Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran ,
Abstract: (116 Views)
Salt stress is a serious environmental threat reducing crop yield. Hence, developing any breeding plan requires an understanding of the basic physiology and cell molecular genetic regulation under salinity stress. In this study, we evaluated the effectiveness of gene expression changes on ion homeostasis comprising salt overly sensitive (SOS1) and vacuolar Na+/H+ antiporter (NHX1) along with ion content measurement and proline content in the rice mutants at Rice Research Institute of Iran in 2018-2019. To survey these realities, tolerant mutant genotypes (em4hs290 and em4hs84) along with Hashemi parent cultivar, IR28 (sensitive), and FL478 (tolerant) seedlings were treated with 100 mM NaCl. Based on the results of growth indices, the seedling length of Hashemi cultivar and IR28 decreased considerably about 44.7%, and 44.2% reduction to that of the control, and the leaves progressively yellowed. Results showed that proline content and K+ and K+/Na+ ratio increased about ~2–3-fold higher in the tolerant genotypes than in the susceptible ones. Also, the overall amount of the OsNHX1 and SOS1 expression increased in tolerant genotypes compared to the susceptible ones. Accordingly, the compatible solute accumulation significantly advanced resulting in improvement of ionic homeostasis and probably suppresses the stress. Moreover, the variable pattern of gene expression in the two salt-tolerant mutants (em4hs290 and em4hs84) and Hashemi parent showed that the induced mutation could increase the salt-tolerant in mutant genotypes through ionic and osmotic homeostasis. Generally, these tolerant mutant genotypes could be applied to develop salt-tolerant varieties in rice breeding programs which can bring on production sustainability.
Article Type: Original Research |
Subject:
Plant Breeding and Genetics
Received: 2022/08/11 | Accepted: 2024/01/1
Received: 2022/08/11 | Accepted: 2024/01/1
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