1- Department of Biology, Faculty of Science, University of Tehran, Islamic Rebublic of Iran.
Abstract: (6020 Views)
Leaf and root explants from 10 to 20 day– old seedlings of Plantago lanceolata, were
cultured on MS, MSH, NT and B5 basal media with various concentrations of different
plant growth regulators. The best callus induction was obtained with 0.4 mgl-1 2,4-D in
MSH I with 400 callus index. Callus growth was significantly stimulated (P=0.05)with 0.8
mgl-1 2,4-D and 0.1 mgl-1 Kin in MSH II, with 799 mg fresh weight and 79 mg dry weight.
A higher production of mucilage was obtained in MSH II. The mucilage content of different
media varied from 10.40 to 14.73% dry weight. Perusal of the data reveals significant
differences with regard to the total mucilage content. Callus has nearly 3 times and 1.5
times more mucilage than seeds and leaf and root parts respectively.
Subject:
Plant Breeding Received: 2010/05/16 | Accepted: 2010/05/16 | Published: 2010/05/16