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1- Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan , r.saberi@vru.ac.ir
2- Department of Genetics and Plant Production, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan
3- Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan
4- Pistachio safety research center, Rafsanjan University of Medical Science, Rafsanjan
Abstract:   (237 Views)
The enzymes play a crucial role in the plant-pathogen interaction and is very important to manage plant diseases. Take-all disease (Gaeumannomyces graminis var. tritici) is a disease of the crown and root rot in wheat and so far, has not been identified the resistance mechanism of this disease.  In this study, 8 bread wheat genotypes were cultured, and the changes were evaluated enzymes of peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase enzymes, total protein in 0, 4, 7, 9, and 12 days after inoculation. The results of this study showed that different genotypes have different reactions to the take-all disease. Based on the average disease intensity, genotypes were divided into three groups: resistant, moderately resistant and susceptible. The results showed that the level of polyphenol oxidase and phenylalanine ammonia-lyase, and total protein increased in the resistant and moderately resistant groups. Cluster analysis by K-means was performed to produce three clusters and genotypes of the second (resistant) and third (moderately resistant) clusters have higher polyphenol oxidase and phenylalanine ammonia-lyase activity, and total protein higher than the first cluster. Multivariate analysis indicated that peroxidase enzyme might influence the resistance indirectly. The results have clarified the role of polyphenol oxidase enzymes and total protein in the development of resistance to take-all disease.
Article Type: Original Research | Subject: Plant Protection/Biological control
Received: 2020/06/11 | Accepted: 2020/07/20

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