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1- Sari Agricultural Sciences and Natural Resources University (SANRU)
2- Research Institute of Biotic Technologies of Medicinal and Aromatic Plants, Sari Agricultural Sciences and Natural Resources University (SANRU), , mehdihadadi@gmail.com
Abstract:   (106 Views)
Background of blackberry cultivars and domestication in wild species is unclear in the south coast of Caspian Sea as an origin of diversity. Genetic diversity of 45 genotypes of thornless and thorny blackberries from the collection of (SANRU) located in south of Caspian Sea were studied by Inter Simple Sequence Repeat (ISSR) molecular markers and Jaccardchr('39')s similarity coefficient used to plot the cluster diagram according to the unweighted pair-group method with arithmetic averages (UPGMA) algorithm. Results showed 10 ISSR primers amplified 345 fragments of which 344 were polymorphic. The average numbers of bands were 34.5 for primers. Based on the PCA results, genotypes were classified in three groups. Some wild genotypes located closed to commercial thorny cultivars. Which showed the native and local people in south of Caspian Sea identified and selected superior genotypes and took step in domestication of wild genotypes, based on their experience and background. Cluster analysis divided the genotypes to six groups. Imported genotypes located in the same group but were separated in sub-groups according to maturity time which include early, mid and late ripen cultivars. These genetical traits separated them and confirmed the morphology marker result which named them as Silvan, Marion and Tupy for thorny cultivars. The result indicated that genepool of thornless blackberry is not limited to chimera type (as first generation of thornlessness) and it probably includes the two further steps in evolution, and even include some new and evolved type of native thornlessness.
Article Type: Original Research | Subject: Pomology
Received: 2019/09/18 | Accepted: 2020/07/20

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