Effect of Sowing Date and Glycinebetaine on Seed Yield, Oil Content, and Fatty Acids in Rapeseed Cultivars

Document Type : Original Research

Authors
N. Safdari-Monfared 1
G. Noor-Mohammadi 1
A. H. Shirani Rad 2
E. Majidi Hervan 1
1 Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
2 Seed and Plant Improvement Institute (SPII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Islamic Republic of Iran.
Abstract
This study primarily aimed to identify and suggest appropriate rapeseed cultivars and their optimum sowing time. A factorial split-plot experiment was conducted in a complete randomized block design with three replications during two years (2014-2016). The study was carried out using six rapeseed cultivars, namely, Elvis, HL2012, L155, KR2, HW113 and Danob, three sowing dates (October 7, 17, and 27), and two concentrations of glycinebetaine (0 as control and 0.2%). The sowing dates and the GlycineBetaine (GB) were allotted to main plots and the six cultivars were allotted to subplots. The HL2012 cultivar had the highest seed yield (4,584 kg ha-1), seed oil content (44.6%) and seed oil yield (2,060 kg ha-1), which were achieved optimally in the first sowing date. Application of GB increased the average seed yield from 4,089 to 4,419 (kg ha-1), seed oil from 44 to 44.4% and seed oil yield from 1,818 to 1,976 (kg/ha). The amounts of proline and soluble carbohydrates in the plants increased from the first to the third sowing dates. The early sowing date and the application of GB had positive effects on the quantity and quality of rapeseed oil. The results of cluster analysis showed that three of the six cultivars could be suitable for cultivation in Karaj, Iran.

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Agboma, P. C., Sinclair, T. R., Jokinen, K., Peltonen-Sainio, P. and Pehu, E. 1997. An evaluation of the effect of exogenous glycinebetaine on the growth and yield of soybean: timing of application, watering regimes and cultivars. Field crop res, 54(1): 51-64.
Asghari, B. A. H., Heravan, E. M., Alizadeh, B., Abad, H. H. S. and Madani, H. 2018. Oil content, seed yield and morphological changes of canola cultivars in response to different sowing dates. Crop Res, 53: 38-44.
Ashraf, M. and Foolad, M. R. 2007. Role of glycinebetaine and proline in improving plant abiotic stress resistance. Environ Exp Bot, 59: 206-216.
Bates, L. S., Waldren, R. P. and Teare, I. D. 1973. Rapid determination of free proline for water-stress studies. Plant soil, 39(1): 205-207.
Bitarafan, Z. and Shirani Rad, A. H. 2012. Water stress effect on spring rapeseed cultivars yield and yield components in winter planting. Int J Phys Sci, 7(19): 2755-2767.
Dawood, M. G. and Sadak, M. S. 2014. Physiological role of glycinebetaine in alleviating the deleterious effects of drought stress on canola plants (Brassica napus L.). Middle East J Agric Res, 3(4): 943-954.
Fathi, G., Siadat, S. A. and Hemaiaty, S. S. 2003. Effect of sowing date on yield and yield components of three oilseed rape varieties. Acta agron hung, 51(3): 249-255.
Ghorbani, A., Zarrinkamar, F. and Fallah, A. 2009. The effect of cold stress on the morphologic and physiologic characters of two rice varieties in seedling stage. J Crop Breed, 3: 50-66.
Hampton, J. G., Boelt, B., Rolston, M. P. and Chastain, T. 2013. Effects of elevated CO2 and temperature on seed quality. JAgric Sci, 151: 154-162.
Hashm, J.J. and Mahmood, B.J. 2016. Effect of sowing dates, rates and their interactions on yield and quality of rapeseed (Brassica napus l.) genotypes. MSc thesis, Univ. of Salahaddin, Erbil, College of Agriculture, Dept of Field Crops.
Hussain, M., Farooq, M., Jabran, K., Rehman. H. and Akram, M. 2008. Exogenous glycinebetaine application improves yield under water-limited conditions in hybrid sunflower. Arch Agron Soil Sci, 54: 557-567.
Irigoyen J.J., Emerich, D.W. and Sanchez-Diaz, M. 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plant, 84: 55-60.
Jahanbakh, S., Godehkahriz, S., Karimzadeh, G., Rastgar-Jazii, F., Mahfoozi, S. and Hosseini-Salekdeh, G. 2009. Influence of vernalization on some physiological characteristics and cold tolerance in two susceptible and tolerant cultivars of bread wheat. Electron J Crop Prod, 2(3): 85-106.
Javadian, N., Karimzadeh, G., Mahfoozi, S. and Ghanati, F. 2010. Cold-induced changes of enzymes, proline, carbohydrates and chlorophyll in wheat. Russian J Plant Physiol, 57(4): 540-547.
Koç, E., İşlek, C. and Üstün, A. S., 2010. Effect of cold on protein, proline, phenolic compounds and chlorophyll content of two pepper (Capsicum annuum L.) varieties. Gazi Uni J Sci, 23(1): 1-6.
Krzymahski, J., Petka, T., Ratajska, I., Byczynska, B. and Krotka, K., 1991. Development of low glucosinolate white mustard {Sinapis alba syn. Brassica hirta). Proceedings of 8th International Rapeseed Congress, Saskatoon (Canada) pp. 1545-1548.
Kumar, S., Singh, R. B. and Shakywar, R. C. 2016. Integrated Management of Downy Mildew of Yellow Sarson. Int J Bio-Resourc Stress Manag, 7(1).
Kumar, A., Singh, P., Gangwar, C. S., Kumar, A., Sharma, S. K., Srivastava, A., & Yadav, A. K., 2018. Maximization of growth, seed yield and quality by adjusting date of sowing and nutrient level in mustard.(Brassica juncea Czern & Coss). J Pharmacogn Phytochem, 7(1): 938-940.
Loof, B. 1960. The agronomy and present position of oil seed crops in Scandinavia. Field Crop Abs, 13: 1-7.
Lopez, C. M. L., Takahashi, H. and Yamazaki, S. 2002. Plant water relations of kidney bean plants treated with NaCl and foliarly applied glycinebetaine. J Agron Crop Sci, 188: 73-80.
Ma, Y. H., Ma, F. W., Zhang, J.K., Li, M.J., Wang, Y.H. and Liang, D. 2008. Effects of high temperature on activities and gene expression of enzymes involved in ascorbate-glutathione cycle in apple leaves. Plant Sci, 175: 761-766.
Mendham, N. J., Shipway P. A. and Scott. R. K. 1981. The effects of seed size, autumn nitrogen and population density on the rapeseed to delay sowing oilseed rape (B. napus). J Agric Sci Camb, 96: 417-28.
Moieni-Korbekandi, Z., Karimzadeh, G. and Sharifi, M. 2014. Cold-induced changes of proline, malondialdehyde and chlorophyll in spring canola cultivars. J Plant Physiol Breeding, 4: 1-11.
Park, E. J., Jeknic, Z. and Chen, T. H. 2006. Exogenous application of glycinebetaine increases chilling tolerance in tomato plants. Plant cell physiol, 47(6): 706-714.
Paul, M. J., Driscoll, S. P. and Lawler, D. W. 1992. Sink-regulation of photosynthesis in relation to temperature in sunflower and rape. J Exp Bot, 43: 147-153.
Porra, R. J., Thompson, W.A. and Kriedemann. P.E. 1989. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectrometry. Biochim Biophys Acta (BBA) Bioenerg, 975: 384-394.
Qureshi, K. M., Chughtai, S., Qureshi, U.S. and Abbasi, N. A. 2013. Impact of exogenous application of salt and growth regulators on growth and yield of strawberry. Pak J Bot, 45: 1179-1186.
Rad, A. H. S., Bitarafan, Z., Rahmani, F., Taherkhani, T., Aghdam, A. M. and Nasresfahani, S. 2015. Effects of planting date on spring rapeseed (Brassica napus L.) cultivars under different irrigation regimes. Turk J Field Crops, 19(2): 153-157.
Ratajczak, K., Sulewska, H. and Szymańska, G. 2017. New winter oilseed rape varieties–seed quality and morphological traits depending on sowing date and rate. Plant Production Science, 20(3): 262-272.
Rashid, M., Hampton, J. G., Trethewey, J. A. K. and Rolston, M. P. 2017. Effect of sowing date on forage rape seed quality. Agron NZ, 47: 55-64.
Rashid, U. and Farooq, A. 2008. Production of biodiesel through optimized alkaline-catalyzed transesterification of rapeseed oil. Fuel, 87: 265-273.
Raza, S. H., Athar, H. R., Ashraf, M. and Hameed, A. 2007. Glycinebetaine-induced modulation of antioxidant enzymes activities and ion accumulation in two wheat cultivars differing in salt tolerance. Environ Exp Bot, 60: 368-376.
Robertson, M. J., Holland, J. F. and Bambach, R. 2004. Response of canola and Indian mustard to sowing date in the grain belt of north-eastern Australia. Aust J Exp Agr, 44(1): 43-52.
Rouhaninia, M., Gerigourian, V., Motalebi-Azar, A. R. and Dezhampour, J. 2006. Investigating the rate of damage and differences in proline level in flower buds of some commercial apricot cultivars in different phenological stages. J Hort Sci Technol, 8(2): 103-112.
Schaewen, A., Stitt, M., Schmidt, R., Sonnewald, U. and Willmitzer, L. 1990. Expression of yeast-derived invertase in the cell wall of tobacco and Arabidopsis plants leads to accumulation of carbohydrate and inhibition of photosynthesis and strongly influences growth and phenotype of transgenic tobacco plants. EMBO J, 9: 3033-3044.
Sharafi, Y., Majidi, M. M., Jafarzadeh, M. and Mirlohi, A. 2015. Multivariate analysis of genetic variation in winter rapeseed (Brassica napus L.) cultivars. J Agr Sci Tech, 17(5): 1319-1331.
Shweta, M. and Agrawal, S. B. 2006. Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L. Braz J Plant Physiol, 18(2): 307-314.
Siadat, S. A. and Hemayati, S. S. 2009. Effect of sowing date on yield and yield components of three oilseed rape varieties. Acta agron hung, 7: 31-35.
Steel, R. G. D. and Torrie. J. H. 1980. Principles and procedures of statistics: A biometrical approach. 2nd ed. Mc Graw-Hill, New York. p. 20-90.
Stitt, M., Schaewen, A. and Willmitzer, L. 1990. Sink regulation of photosynthetic metabolism in transgenic tobacco plants expressing yeast invertase in their cell wall involves a decrease of the calvin cycle enzymes and an increase of glycolytic enzymes. Planta, 183: 40-50.
Taize, L. and Zeiger, E. 2006. Plant Physiology, 4th ed.; Sinauer Associates, Inc.:Sunderland, MA,USA.
Taylor, A. J. and Smith, C. J. 1992. Effect of sowing date and seeding rate on yield and yield components of irrigated canola (B. napus) grown on a red-brown earth eastern Australia. Aust J Agric Res, 43(7): 1629-41.
Thurling, N. 1974. Morphophysiological determinants of yield in rapeseed. Aust J Agric Res, 25: 711-721.
Trethewey, J. A. K. 2012. Crop management strategies to improve forage rape seed yield. P AG SOC NZ, 42: 111-117.
Turhan, H., GÜL, M. K., Egesel, C. Ö. and Kahriman, F. 2011. Effect of sowing time on grain yield, oil content, and fatty acids in rapeseed (Brassica napus subsp. oleifera). Turk J Agri Forest, 35(3): 225-234.
Uzun, B., Zengin, U., Furat, S. and Akdesir. O. 2009. Sowing date effects on growth, flowering, seed yield and oil content of canola cultivars. Asian Journal of Chemistry, 21: 1957-1965.
Whitefield, D. M. 1992. Effect of temperature and ageing on CO2 exchange of pods of oilseed rape. Field Crop Res, 28(4): 271-280.
Whitfield, D. M. 1992. Effects of temperature and ageing on CO2 exchange of pods of oilseed rape (Brassica napus). Field Crop Res, 28(4): 271-280.
Xia, J. and Wishart. D. S. 2016. Using MetaboAnalyst 3.0 for comprehensive metabolomics data analysis current protocols in bioinformatics. Curr Protoc Bioinformatics, 55: 14-10.
Yang, M., Shi, L., Xu, F.S., J. Lu, W. and Wang, Y.H. 2009. Effects of B, Mo, Zn and their interactions on seed yield of rapeseed (Brassica napus L.). Pedosphere, 19(1): 53-59.
Yau, S. K. 2007. Winter versus spring sowing of rain-fed safflower in a semi-arid, high-elevation Mediterranean environment. Eur J Agron, 26: 249-256.
 
Journal of Agricultural Science and Technology
Volume 21, Issue 6
September and October 2019
Pages 1495-1506