Volume 21, Issue 5 (2019)                   JAST 2019, 21(5): 1287-1299 | Back to browse issues page

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Goldasteh M, Mehregan I, Naghavi M R, Nejadsattari T. Molecular Characterization of Low Molecular Weight Glutenin (LMW) Genes in Triticeae Species with D Genome. JAST. 2019; 21 (5) :1287-1299
URL: http://jast.modares.ac.ir/article-23-21596-en.html
1- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran. , goldasteh1390@gmail.com
2- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
3- Department of Agronomy and Plant Breeding, University of Tehran, Karaj, Islamic Republic of Iran.
Abstract:   (1380 Views)
Low Molecular Weight Glutenin Subunits (LMW-GS), encoded by GLU-3 loci located on the short arm of homologous chromosomes of wheat, play an important role in the bread making quality. Some of the most important gens for quality are located on the D genome, which are interesting in wheat breeding programs. In addition to the bread wheat (Triticum​​​​​​​ aestivum), some species of Aegilops including Ae. cylindrica, Ae. tauschii, Ae. crassa, Ae. juvenalis, and Ae. vavilovi carry D genome. In this study, the phylogenetic relationship among Aegilops species with D genome and bread wheat has been studied based on the sequence of low molecular weight glutenins loci. The results indicated a great diversity for these loci. Presence of several numbers of common protein bands among species suggested a close relationship and high genetic flow among species. Three primers for the LMW-GS proteins were able to reveal the relationship between the species. The results showed a close relationship among bread wheat (T. astivum) and Ae. tauschii species. Ae. crassa species is more distant from bread wheat. Also, the results indicated a close relationship between the Ae. cylindrica, Ae. juvenalis, and Ae. vavilovi. A great diversity of LMW-GS in wild relatives and close relationship between these species and wheat suggest them as a potential source of genes for wheat breeding programs.
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Article Type: Original Research | Subject: Bioinformatics
Received: 2018/06/1 | Accepted: 2018/11/4 | Published: 2019/09/15

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