Volume 6, Issue 1 (2004)
JAST 2004, 6(1): 57-62 |
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Extraction and separation of Antioxidative Compounds
from Salvia leriifolia Leaves. JAST 2004; 6 (1) :57-62
URL: http://jast.modares.ac.ir/article-23-1485-en.html
URL: http://jast.modares.ac.ir/article-23-1485-en.html
Abstract: (5120 Views)
In this research, methanolic extraction of Salvia leriifolia leaves produced a higher yield and antioxidative activity than other organic solvents (ethanol, acetone, chloroform, n-hexane). The methanolic extract was reserved about 24 hours in the refrigerator and its precipitates were then separated. The extract was separated into 12 fractions by thin-layer chromatography (TLC). The highest yields were found in fractions with Rf values of 0.29, 0.54, 0.11 and 0.38 at 16.24%, 12.48%, 8.81% and 7.60%, respectively. All fractions and also whole methanolic extract and precipitates of methanolic extract had more anti-oxidative activity than the control based on the thiocyanate method. Whole methanolic ex-tract, precipitates of methanolic extract and most separated fractions showed more anti-oxidative activity than –tocopherol. The fraction with Rf value of 0.29 at 16.24% yield and 85.61% antioxidative activity of synthetic antioxidant BHT based on thiocyanat method was chosen as the fraction with the highest yield and the highest antioxidative ac-tivity.
Keywords: Antioxidant activity, Salvia
Subject:
Horticultural Science
Received: 2010/02/8 | Accepted: 2010/02/8 | Published: 2010/02/8
Received: 2010/02/8 | Accepted: 2010/02/8 | Published: 2010/02/8
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