Application of Classification Tree Method to Determine Factors Affecting Somatic Cell Count in Holstein Cows

Authors
1 Department of Animal Science, Faculty of Agricultural Science, Payame Noor University, Tehran, Islamic Republic of Iran.
2 Department of Animal Science, College of Agriculture, Isfahan University of Technology, Isfahan, Islamic Republic of Iran.
3 Department of Animal Science, Faculty of Agricultural Science, Lorestan University, Khorramabad, 68159 Lorestan, Islamic Republic of Iran.
4 Department of Biotechnology and Animal Genetics, Faculty of Biology and Animal Breeding, UTP University of Science and Technology in Bydgoszcz, Poland.
Abstract
In the current study, the effect of phenotypic factors on Somatic Cell Count (SCC) was evaluated by using classification tree technique. The current study used a total of 1,972,031 test day records of SCC in parity 1 to 4 collected from 1,281 Iranian Holstein-Friesian cows’ herds through 2004–2013. The SCC records were converted to binary trait, defined as 1: If SCC≤ 200,000, and 2: Otherwise. The CART (Classification And Regression Tree) algorithm for classification trees, with GINI index and Entropy function as the division criteria, was used to develop the tree. Statistical analysis was performed using ‘rpart’ package in R software. The constructed tree had 12 leaves and it was 6 levels deep. The results of classification tree procedure for ranking of importance of the variables responsible for the variation in SCC were, respectively, parity, test-day milk production, year of calving, season of calving, and days in milk (stage of lactation). Based on the obtained classification tree, different combination of variables associated with SCC could be identified. According to the classification tree, the lowest amount of SCC was expected in the group of cows that were in the 1st or 2nd parity; their test-day milk production was > 30 kg; they were calved from year 2004 to 2013; and their calving season was autumn or winter.

Keywords

Subjects


1. Banos, G. and Shook, G. E. 1990. Genotype by Environment Interaction and Genetic Correlations among Parities for Somatic Cell Count and Milk Yield. J. Dairy Sci., 73: 2563–2573.
2. Beck, H. S., Wise, W. S. and Dodd F. H. 1992. Cost Benefit Analysis of Bovine Mastitis in the UK. J. Dairy Res., 59: 449–460.
3. Boettcher, P. J., Hansen, L. B. and Ernst, C. A. 1992. Genetic Evaluation of Holstein Bulls for Somatic Cells in Milk of Daughters. J. Dairy Sci., 75: 1127–1137.
4. Breiman, L., Friedman, J., Stone, C. J. and Olshen, R. A. 1984. Classification and Regression Trees. CRC Press, Chapman and Hall, New York, USA.
5. Cengiz, M., Kaynar, O., Cannazik, O., Ileriturk, M., Cengiz, S. and Hayirli, A. 2015. Sampling Factors Causing Variability in Milk Constituents in Early Lactation Cows. Vet. Med-Czech, 60: 6–15.
6. De Vliegher, S., Barkema, H. W., Stryhn, H., Opsomer, G. and de Kruif, A. 2004. Impact of Early Lactation Somatic Cell Count in Heifers on Somatic Cell Counts over the First Lactation. J. Dairy Sci., 87: 3672–3682.
7. Detilleux, J., Leroy, P. and Volckaert, D. 1997. Alternative Use of Somatic Cell Counts in Genetic Selection for Mastitis Resistance, Interbull Bull., 15: 34–44.
8. Faraji-Arugh, H., Aslami-Nejhad, A. A. and Rokuyi, M. 2012. The Genetic and Phenotypic Trend and Environmental Factor Affecting Somatic Cell Score in Cattle Iranian Holstein, Iran. J. Anim. Sci. Res., 3: 459–454. (in Farsi)
9. Green, M. J., Bradley, A. J., Newton, H. and Browne, W. J. 2006. Seasonal Variation of Bulk Milk Somatic Cell Counts in UK Dairy Herds: Investigations of the Summer Rise. Prev. Vet. Med., 74: 293–308.
10. Hagnestam, C., Emanuelson, U. and Berglund, B. 2007. Yield Losses Associated with Clinical Mastitis Occurring in Different Weeks of Lactation. J. Dairy Sci., 90: 2260–2270.
11. Harmon, R. J. 1994. Physiology of Mastitis and Factors Affecting Somatic Cell Counts. J. Dairy Sci., 77: 2103–2112.
12. Koc, A. and Kizilkaya, K. 2009. Some Factors Influencing Milk Somatic Cell Count of Holstein Friesian and Brown Swiss Cows under the Mediterranean Climatic Conditions. Arch. Tierz., 52: 124–133.
13. Koivula, M., Mäntysaari, E. A., Negussie, E. and Serenius, T. 2005. Genetic and Phenotypic Relationships among Milk Yield and Somatic Cell Count before and after Clinical Mastitis. J. Dairy Sci., 88: 827–833.
14. Looper, M. 2012. Reducing Somatic Cell Count in Dairy Cattle. Division of Agriculture Research and Extension, University of Arkansas System, FSA4002, 2012, Available at: http://www.uaex.edu/publications /pdf /fsa-4002.pdf
15. Ma, Y., Ryan, C., Barbano, D. M., Galton, D. M., Rudan, M. A. and Boor, K. J. 2000. Effects of Somatic Cell Count on Quality and Shelf-Life of Pasteurized Fluid Milk. J. Dairy Sci., 83: 264–274.
16. Monardes, H. G., Kennedy, B. W. and Moxley, J. E. 1983. Heritabilities of Measures of Somatic Cell Count per Lactation. J. Dairy Sci., 66: 1707–1713.
17. Norman, H. D., Lombard, J. E., Wright, J. R., Kopral, C. A., Rodriguez, J. M. and Miller, R. H. 2011. Consequence of Alternative Standards for Bulk Tank Somatic Cell Count of Dairy Herds in the United States. J. Dairy Sci., 94: 6243–6256.
18. Ødegård, J., Heringstad, B. and Klemetsdal, G. 2004. Short Communication: Bivariate Genetic Analysis of Clinical Mastitis and Somatic Cell Count in Norwegian Dairy Cattle. J. Dairy Sci., 87: 3515–3517.
19. Ødegard, J., Klemetsdal, G. and Heringstad, B. 2003. Variance Components and Genetic Trend for Somatic Cell Count in Norwegian Cattle. Livest. Prod. Sci., 79: 135–144.
20. Østergaard, S., Chagunda, M. G. G., Friggens, N. C., Bennedsgaard, T. W. and Klaas, I. C. 2005. A Stochastic Model Simulating Pathogen-Specific Mastitis Control in a Dairy Herd. J. Dairy Sci., 88: 4243–4257.
21. Pagnacco, G., Miglior, F., Zhang, W. C., Dekkers, J. C. M. and Burnside E. B. 1994. Genetic Evaluation for Somatic Cell Count and Relationship with Inbreeding in Canadian Holstein. In Proceedings of the World Congress on Genetics Applied to Livestock Production, 7–12 August, Guelph, Canada, 93 PP.
22. Piwczyński, D. and Sitkowska, B. 2012. Statistical Modelling of Somatic Cell Counts Using the Classification Tree Technique, Arch. Tierz., 55: 332–345.
23. Sadeghi-Sefidmazgi, A., Moradi-Shahrbabak, M., Nejati-Javaremi, A., Miraei-Ashtiani, S. R. and Amer, P. R. 2011. Estimation of Economic Values and Financial Losses Associated with Clinical Mastitis and Somatic Cell Score in Holstein Dairy Cattle. Animal, 5: 33–42.
24. Sargeant, J. M., Schukken, Y. H. and Leslie, K. E. 1998. Ontario Bulk Milk Somatic Cell Count Reduction Program: Progress and Outlook. J. Dairy Sci., 81: 1545–1554.
25. Schukken, Y. H., Leslie, K. E., Weersink, A. and Martin, S. W. 1992. Ontario Bulk Milk Somatic Cell Count Reduction Program. 1. Impact on Somatic Cell Counts and Milk Quality. J. Dairy Sci., 75: 3352–3358.
26. Sharma, N., Singh, N. K. and Bhadwal, M. S. 2011. Relationship of Somatic Cell Count and Mastitis: An Overview. Asian Austral. J Anim., 24: 429–438.
27. Singh, M. and Ludri, R. S. 2001. Influence of Stages of Lactation, Parity and Season on Somatic Cell Counts in Cows. Asian Austral. J Anim., 14: 1775–1780.
28. Skrzypek, R., Wojtowski, J. and Fahr, R. D. 2004. Factors Affecting Somatic Cell Count in Cow Bulk Tank Milk: A Case Study from Poland. J. Vet. Med. A, 51: 127–131.
29. Sordillo, L. M., Shafer-Weaver, K. and DeRosa, D. 1997. Immunobiology of the Mammary Dland. J. Dairy Sci., 80: 1851–1865.
30. Tančin, V. 2013. Somatic Cell Counts in Milk of Dairy Cows under Practical Conditions. Slovak J. Anim. Sci., 46: 31–34.
31. Vallimont, J. E., Dechow, C. D., Sattler, C. G. and Clay, J. S. 2009. Heritability Estimates Associated with Alternative Definitions of Mastitis and Correlations with Somatic Cell Score and Yield. J. Dairy Sci., 92: 3402–3410.
32. Yamazaki, T., Hagiya, K., Takeda, H., Sasaki, O., Yamaguchi, S., Sogabe, M., Saito, Y., Nakagawa, S., Togashi, K., Suzuki, K. and Nagamine, Y. 2013. Genetic Correlations between Milk Production Traits and Somatic Cell Scores on Test Day within and across First and Second Lactations in Holstein Cows. Livest. Sci., 152: 120–126.