Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

---

Thymus is a medicinal plant which contains one of the world's top ten essential oils, exhibiting antibacterial, antioxidative, food preservative and mammalian age-delaying properties. This work was aimed at identifying between-species variations requiring for selecting appropriate parents for hybridization. Six Iranian endemic Thymus accessions belonging to Thymus daënensis, T. eriocalyx and T. migricus were studied. Root tips were examined for karyological studies and fresh young leaves of the standard reference (Parsley, Petroselinum crispum, 2C DNA= 4.45 pg) and the Thymus samples stained with propidium iodide (PI) for flow cytometric (FCM) measurements. Two ploidy levels (diploid and tetraploid) and 3 chromosome numbers (30, 56, 60) were recognized. The latter chromosome number is being reported for the first time on T. daënensis accession. FCM measurements showed that 2C DNA contents varied from 1.02 to 2.42 pg, verifying more than 2-fold variations and showing a genome size range of 499 to 1182 Mbp, correspondingly. The mean amount of 2C DNA/chromosome and mean of monoploid genome size were not proportional to ploidy. 2C-values were correlated with, and linearly regressed upon somatic metaphase, considering either total chromosome volume (TCV) or total chromatin length (X).

Keywords: Chromosome, DNA C-value, Genome size, Medicinal plant, Thymus daënensis, T. eriocalyx, T. migricus

Full-Text [PDF 508 kb]   (5695 Downloads)    

Add your comments about this article : Your username or Email:

---