Volume 21, Issue 2 (2019)                   JAST 2019, 21(2): 475-485 | Back to browse issues page

‎ 20.1001.1.16807073.2019.21.2.2.3

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Abdelkhalek A, Sanan-Mishra N. Differential Expression Profiles of Tomato miRNAs Induced by Tobacco Mosaic Virus. JAST 2019; 21 (2) :475-485
URL: http://jast.modares.ac.ir/article-23-13577-en.html
Differential Expression Profiles of Tomato miRNAs Induced by Tobacco Mosaic Virus
A. Abdelkhalek * 1, N. Sanan-Mishra2
1- Plant Protection and Bimolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, 21934, Alexandria, Egypt.
2- Plant RNAi Biology Group, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
Abstract:   (3166 Views)
Plant microRNAs (miRNAs) play important roles in plant development and responses to biotic and abiotic stress. Recently, there is clear evidence that miRNAs are involved in host-virus interactions. By using stem-loop RT-PCR, an expression levels change of thirteen miRNA belonging to six miRNA families targeting leaf development and morphogenesis were analyzed upon Tobacco Mosaic Virus (TMV)-tomato infection. Compared to mock plants, significant changes in relative expression levels of nine miRNAs were observed. The miR319c-5p showed the highest statistically significant increase in accumulation at 15 days post-inoculation. At all time points tested, miR159, miR164a-3p, miR164a-5p, miR166c-5p and miR319c-5p were up-regulated while miR160, miR319a, miR319b, miR319c-3p were down-regulated in most cases. Our data could provide new insights into the role of miRNAs in tomato-TMV interaction and in developing efficient strategies for improving tomato resistance against viral infection.
 
Keywords: Host-virus interaction, Stem-loop, RT-PCR, TMV, Viral infection.
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Article Type: Research Paper | Subject: Agricultural Economics/Agriculture Marketing and Supply Chains
Received: 2017/02/19 | Accepted: 2018/08/28 | Published: 2019/03/2
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