Volume 16, Issue 1 (2014)
JAST 2014, 16(1): 191-202 |
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Sarvestani R, Peyghambary S A, Abbasi A. Isolation and Characterization of DBR2 Gene Promoter from Iranian Artemisia annua. JAST 2014; 16 (1) :191-202
URL: http://jast.modares.ac.ir/article-23-12139-en.html
URL: http://jast.modares.ac.ir/article-23-12139-en.html
1- Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj, Islamic Republic of Iran.
Abstract: (6883 Views)
Artemisia annua is still the only commercial source of Artemisinin. To date, a number of biochemical and molecular studies about Artemisinin’s biosynthetic pathway have been carried out. In metabolic engineering approach, isolation and characterization of promoters leads to an understanding of which cis-acting elements are responsible for the regulation of gene expression. DBR2 is a key enzyme in Artemisinin biosynthetic pathway. In order to allow chromosome walking beyond the 5'-flanking region of DBR2, two specific primers were used in combination with 6 arbitrary primers in TAIL-PCR method. A 696bp upstream of DBR2 start codon was isolated and cloned. The subsequent sequence analysis using bioinformatics softwares revealed that there were several cis-acting elements such as TATA-box, CAAT-box, and MeJA-responsive element, and several W-box and light-responsive elements inside the DBR2 promoter. These results can be helpful in understanding of artemisinin biosynthesis regulation and will facilitate metabolic engineering of the compound.
Received: 2012/09/2 | Accepted: 2013/11/4 | Published: 2014/01/1
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