Volume 17, Issue 5 (2015)
JAST 2015, 17(5): 1303-1317 |
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Pouresmael M, Mozafari J, Khavari-Nejad R A, Najafi F, Moradi F. Identification of Possible Mechanisms of Chickpea (Cicer arietinum L.) Drought Tolerance Using cDNA-AFLP. JAST 2015; 17 (5) :1303-1317
URL: http://jast.modares.ac.ir/article-23-11469-en.html
URL: http://jast.modares.ac.ir/article-23-11469-en.html
1- Department of Plant Physiology, Faculty of Biological Sciences, Kharazmi University, 15719-14911, Tehran, Islamic Republic of Iran.
2- National Plant Gene Bank, Seed and Plant Improvement Institute, Mahdasht Avenue, Karaj, Islamic Republic of Iran.
3- Department of Molecular Physiology, Agriculture Biotechnology Research Institute, Karaj, Islamic Republic of Iran.
2- National Plant Gene Bank, Seed and Plant Improvement Institute, Mahdasht Avenue, Karaj, Islamic Republic of Iran.
3- Department of Molecular Physiology, Agriculture Biotechnology Research Institute, Karaj, Islamic Republic of Iran.
Abstract: (6394 Views)
Drought sensitivity is considered as a major concern for chickpea (C. arietinum) seed production. Determination of drought adaptation mechanisms is an essential constituent of this crop breeding programs. With this purpose, the present research was conducted to distinguish the molecular basis of chickpea drought tolerance using cDNA-AFLP approach. The expression profile was compared between drought tolerant (ICCV2 and FLIP9855C) and susceptible lines (ILC3279) of chickpea under three drought treatments including well-watered, intermediate, and severe stress; where soil water content was kept at 85–90%, 55–60%, and 25–30% of Field capacity, respectively. Totally, 295 transcript-derived fragments (TDFs) were visualized. Among the differentially expressed TDFs, 72 TDFs were sequenced. Sequenced cDNAs were categorized in different functional groups involved in macromolecules metabolism, cellular transport, signal transduction, transcriptional regulation, cell division and energy production. Based on the results, ribosomal protein S8, mitochondrial chaperone, proteases, hydrolases, UDP -glucuronic acid decarboylase, 2-hydroxyisoflavanone dehydratase, NADPH dehydrogenase, chloride channels, calmodulin, ABC transporter, histone deacetylase and factors involved in chloroplast division were among genes that were affected by drought stress. Similarity search in data base showed that cell wall elasticity, isoflavonoids, maintenance of structure and function of proteins through increase in expression of mitochondrial chaperones, programed cell death, and remobilization of storage material from leaves to seeds were among mechanisms that distinguished differences between drought tolerant and drought susceptible lines.
Keywords: Drought Stress, cDNA- amplified fragments length polymorphism, Transcript derived fragments (TDFs), Gene expression
Article Type: Research Paper |
Subject:
Plant Breeding
Received: 2014/01/22 | Accepted: 2014/09/21 | Published: 2015/09/1
Received: 2014/01/22 | Accepted: 2014/09/21 | Published: 2015/09/1
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