Effect of Preslaughter Shackling on Stress, Meat Quality Traits, and Glycolytic Potential in Broilers

Authors
E. Dereli Fidan 1
S. Ünübol Aypak 2
M. K. Turkyilmaz 1
A. Nazligul 1
S. Karaarslan 1
1 Department of Animal Science, Faculty of Veterinary Medicine, Adnan Menderes University, Turkey.
2 Department of Biochemistry, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin-09016, Turkey.
Abstract
The aim of this study was to investigate the effects of preslaughter shackling durations on some blood parameters, breast and thigh meat quality traits, and muscle metabolites in broilers. The effects of shackling were determined in a group of Ross 308 broilers (240 birds) aged 42 days. Four shackling treatments were used in experimental tests: shackling of broilers for 10 (Group G10; as control), 30 (G30), 60 (G60), and 120 seconds (G120). Results showed that corticosterone (CORT) level (2314.79 pg ml-1) at 120 seconds shackling group increased (P< 0.01). Results indicated that kinase (CK) activity was the highest (2265.69 U I-1) in the 120 seconds shackling group while it was the lowest (1970.64 U I-1) in 10 s group according to the shackling duration (P< 0.05). The breast meat redness value increased due to increase in shackling duration (P< 0.05). It was revealed that shackling duration had decreased breast muscle glycogen level in all treatment groups (P< 0.001). Conversely, breast lactate level increased according to increase in shackling duration (P< 0.05). It was revealed that there was a negative relationship (r= -0.466) between breast meat ultimate pH and cooking loss (CL) value in male broilers (P< 0.01). These results indicated that the preslaughter shackling procedure might be a considerably stressful procedure for broilers, particularly exceeding 60 s. This study suggested that broilers could be at disadvantage due to more struggle during long duration shackling and accelerated postmortem glycolysis, which is detrimental to the quality of breast meat.

Keywords

1. Anadon, H. L. S. 2002. Biological, Nnutritional, and Processing Factors Affecting Breast Meat Quality of Broilers. PhD Thesis, Virginia Polytechnic Institute and State University, Blacksburg, Virginia.
2. Bedanova, I., Voslarova, E., Chloupek, P., Pistekova, V., Suchy, P., Blahova, J., Dobsikova, R. and Vecerek, V. 2007. Stress in Broilers Resulting from Shackling. Poult. Sci., 86: 1065-1069.
3. Bee, G., Biolley, C., Guex, G., Herzog, W., Lonergan, S. M. and Huff-Lonergan, E. 2006. Effects of Available Dietary Carbohydrate and Preslaughter Treatment on Glycolytic Potential, Protein Degradation, and Quality Traits of Pig Muscles. J. Anim. Sci., 84: 191-203.
4. Berri, C., Debut, M., Sante-Lhoutellier, V., Arnould, C., Boutten, B., Sellier, N., Baezaa, E., Jehl, N., Jego, Y., Duclos, M. J. and Le Bihan-Duval, E. 2005. Variations in Chicken Breast Meat Quality: Implications of Struggle and Muscle Glycogen Content at Death. Br. Poult. Sci., 46: 572-579.
5. Berri, C., Le Bihan-Duval, E., Debut, M., Sante-Lhoutellier, V., Baeza, E., Gigaud, V., Jego, Y. and Duclos, M. J. 2007. Consequence of Muscle Hypertrophy on Characteristics of Pectoralis Major Muscle and Breast Meat Quality of Broiler Chickens. J. Anim. Sci., 85: 2005-2011.
6. Berri, C., Wacrenier, N., Millet, N. and Le Bihan-Duval, E. 2001. Effect of Selection for Improved Body Composition on Muscle and Meat Characteristics of Broilers from Experimental and Commercial Lines. Poult. Sci., 80: 833-838.
7. Debut, M., Berri, C., Arnould, C., Guemene, D., Sante-Lhoutellier, V., Sellier, N., Baeza, E., Jehl, N., Jego, Y., Beaumont, C. and Le Bihan-Duval, E. 2005. Behavioral and Physiological Responses of Three Chicken Breeds Stress Pre-slaughter Shackling and Acute Heat Stress. Br. Poult. Sci., 46: 527-535.
8. Debut, M., Berri, C., Baeza, E., Sellier, N., Arnould, C., Guemene, D., Jehl, N., Boutten, B., Jego, Y., Beaumont, C. and Le Bihan-Duval, E. 2003. Variation of Chicken Technological Meat Quality in Relation to Genotype and Pre-slaughter Stress Conditions. Poult. Sci., 82: 1829-1838.
9. Duncan, D. B. 1955. Multiple Range and Multiple F Tests. Biometrics, 11: 1-42.
10. Hartschuh, J. K., Novakofski, J. and McKeith, F. K. 2002. Practical Aspects of the Glycolytic Potential Assay. Proc. 55th Annual Reciprocal Meat Conference, American Meat Science Association, Michigan, USA, PP. 39-42.
11. Honikel, K. O. 1998. Reference Methods for the Assessment of Physical Characteristics of Meat. Meat Sci., 49: 447-457.
12. Kannan G., Heath J. L., Wabeck C. J. and Mench J. A. 1997. Shackling of Broilers: Effects on Stress Responses and Breast Meat Quality. Br. Poult. Sci., 38: 323–332.
13. Lopez, K. P., Schilling, M. W. and Corzo, A. 2011. Broiler Genetic Strain and Sex Effects on Meat Characteristics. Poult. Sci., 90: 1105-1111.
14. Mitchell, M. A., Kettlewell, P. J., Maxwell and M. H. 1992. Indicators of Physiological Stress in Broiler Chickens during Road Transportation. Anim. Welfare., 1: 91-103.
15. Monin, G. and Sellier, P. 1985. Pork of Low Technological Quality with a Normal Rate of pH Fall in the Intermediate Post Mortem Period: The Case of the Hampshire Breed. Meat Sci., 13: 49-63.
16. Nijdam, E., Delezie, E., Larnbooij, E., Nabuurs, M. J. A., Decuypere, E. and Stegeman, J. A. 2005. Comparison of Bruises and Mortality, Stress Parameters, and Meat Quality in Manually and Mechanically Caught Broilers. Poult. Sci., 84: 467-474.
17. Salakova, A., Strakova, E., Valkova, V., Buchtova, H. and Steinhauserova, I. 2009. Quality Indicators of Chicken Broiler Raw and Cooked Meat Depending on Their Sex. Acta Vet. Brno., 78: 497-504.
18. Schneider, B. L., Renema, R. A., Betti, M., Carney, V. L. and Zuidhof, M. J. 2012. Effect of Holding Temperature, Shackling, Sex, and Age on Broiler Breast Meat Quality. Poult. Sci., 91: 468-477.
19. Schneider, B. L. 2009 On-farm and Ante Mortem Factors Affecting Broiler Quality. MSc. Thesis, University of Alberta, Edmonton.
20. Statistical Packages for the Social Sciences. 2006. SPSS Base 15.0 User’s Guide. SPSS, Chicago, IL.
21. Winnicka, A. 1997. Reference Values of Basic Laboratory Tests in Veterinary Medicine. 1st Edition, SGGW Publishers, Warszawa, 56 PP.
22. Yetişir, R., Karakaya, M., İlhan, F., Yılmaz, M. T. and Özalp, B. 2008. Effects of Different Lighting Programs and Sex on Some Broiler Meat Quality Properties Affecting Consumer Preference. Hayvansal Üretim., 49: 20-28.
23. Zar, J. H. 1999. Biostatistical Analysis. 4th Edition, Prentice Hall, NJ, 662 PP.
24. Zhang, L., Yue, H. Y., Zhang, H. J., Xu, L., Wu, S. G., Yan, H. J., Gong, Y. S. and Qi, G. H. 2009. Transport Stress in Broilers. I. Blood Metabolism, Glycolytic Potential, and Meat Quality. Poult. Sci., 88: 2033-2041.
Journal of Agricultural Science and Technology
Volume 17, Issue 5
September and October 2015
Pages 1141-1150