Fruit Research institute, Čačak, Republic of SerbiaDepartment of Pomology and Fruit Breeding , sradicevic@institut-cacak.org
Abstract: (96 Views)
The primary objective of the research was to reveal effect of cryotreatment on pollen reproductive ability in vivo by monitoring pollen tubes growth in sweet cherry (Prunus avium L.) pistils. The influence of cryotreatment was considered from the context of its interaction to polleniser genotype, and possibility for usage in vivo parameters for assessment of pollen fertilization ability after cryopreservation. Pistils of sweet cherry cultivar ‘Regina’ were pollinated with both cryotreated and non-cryotreated pollen of ‘Kordia’ and ‘Summit’. Quantitative parameters of pollen tube growth in vivo (pollen tube number in the certain pistil parts, pollen tube dynamics and fertilization percentage) were determined using fluorescence microscopy method, and obtained data were analyzed together with those obtained for in vitro pollen performance and fruit set. The research have indicated that: i) reproductive ability of cryotreated sweet cherry pollen is verifiable analyzing in vivo parameters, which should be combined with in vitro test; ii) semi- or fully-compatible female recipients, whose sporophytes are supportive for male gametophytes should be used for the assessment; iii) the key parameters for in vivo testing are pollen tube number in the upper part of the style and in the ovary, as well as fertilization percentage, i.e. the percentage of pistils with penetration of the longest pollen tube in the nucellus of the ovary on the 10th day after pollination. Therefore, in vivo testing of the pollen reproductive ability after cryopreservation can give the answers about pollen quality with valuable credibility, in maximum two weeks after thawing and rehydration.
Article Type: Original Research |
Subject:
Plant Breeding and Genetics
Received: 2023/10/4 | Accepted: 2024/01/11 | Published: 2024/03/31
Received: 2023/10/4 | Accepted: 2024/01/11 | Published: 2024/03/31
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