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This study was conducted to investigate the effect of mineral (F) and Mineral-organic Fertilizers (MF) on soil CO₂ flux during winter in grassland and cornfield in Southern Hokkaido, Japan, from May 2013 to April 2014. CO₂ flux was measured by the static chamber method. Soil CO₂ concentration was determined using silicone tubes pipe. The environmental variables, i.e. climate and soil, were also analyzed in this study. Results showed that, in freezing period, CO₂ flux in MF was lower than F plots in grassland (0.1 and 0.4 Mg C ha^-1 period^-1, respectively). However, in melting period, CO₂ flux in F was lower than MF plots (0.01 and 0.1 Mg C ha^-1 period^-1, respectively).  CO₂ flux in F and MF plot was similar in cornfield in freezing (0.5 Mg C ha^-1 period^-1) and melting (0.01 Mg C ha^-1 period^-1). These results were opposite to the annual CO₂ flux where MF was higher than F plot in both grassland (10.9 and 8.5 Mg C ha^-1 period^-1, respectively) and cornfield (8.7 and 6.2 Mg C ha^-1 period^-1, respectively). Soil CO₂ concentration during winter was relatively higher in grassland than cornfield. Soil NH₄-N and Water Extractable Organic Carbon (WEOC) showed a positive correlation with soil CO₂ concentration   (r²= 0.39 and r²= 0.19, respectively). On the other hand, a negative correlation was observed between soil CO₂ concentration and soil NO₃-N content (r²= -0.39).
 

Keywords: Carbon dioxide emission, Green-house gasses, Land use, Manure, Plant nutrient.

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