Volume 17, Issue 5 (2015)                   JAST 2015, 17(5): 1267-1277 | Back to browse issues page

‎ 20.1001.1.16807073.2015.17.5.1.0

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Gholamzadeh khoei S, Abdollahi Mandoulakani B, Bernousi I. Genetic Diversity in Iranian Melon Populations and Hybrids Assessed by IRAP and REMAP Markers. JAST 2015; 17 (5) :1267-1277
URL: http://jast.modares.ac.ir/article-23-139-en.html
Genetic Diversity in Iranian Melon Populations and Hybrids Assessed by IRAP and REMAP Markers
S. Gholamzadeh khoei1 , B. Abdollahi Mandoulakani 1, I. Bernousi1
1- Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Islamic Republic of Iran.
Abstract:   (6069 Views)
Retrotransposons (RTNs) constitute informative molecular markers for plant species because of their ability to integrate into a multitude of loci throughout the genome and thereby generate insertional polymorphisms between individuals. In the present study, RTN-based molecular markers, IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism), were applied to study RTN integration events and genetic diversity in 100 melon genotypes (88 genotypes from 11 populations, three inbred lines, and 9 hybrids). A total of 94 and 262 loci were amplified using 5 IRAP and 15 REMAP primers, respectively. The percentage of polymorphic loci (PPL) in populations ranged from 39% (Zivari Shahrood) to 48% (Shadegani E). The Mantel test between IRAP and REMAP cophenetic matrices evidenced no significant correlation (r= 0.29). IRAP+REMAP-based cluster analysis using UPGMA algorithm and Dice similarity coefficient depicted 6 groups among 100 melon genotypes. AMOVA revealed the higher level of genetic variation within populations (67%) compared to among populations (33%). The mean Fst values of all groups, except for group VI, were more than 0.20, demonstrating differentiation among the populations and genetic structure of the studied melon collection. 
Keywords: Cucumis melo, Genetic variability, Reme1, Retrotransposon
Full-Text [PDF 517 kb]   (8457 Downloads)    
Article Type: Research Paper | Subject: Horticultural Science
Received: 2014/05/17 | Accepted: 2014/11/23 | Published: 2015/09/1
Add your comments about this article : Your username or Email:
CAPTCHA
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.