Volume 16, Issue 89 (7-2019)
Abstract
Doogh is one of the most used fermented dairy drinks in Iran, whose durability and taste in the food industry is very important. The aim of this study was to investigate the antimicrobial effect of hydroalcoholic extract of salvia (Salvia officinalis), basil (Ocimum basilicum) and cinnamon on the durability of dough at ambient temperature.
Hydroalcoholic extracts of salvia, basil and cinnamon were extracted by maceration method. The antimicrobial effects of the extract of these three plants were determined by microdilution on yeast of Saccharomyces cerevisiae by determining minimum microbial inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), and then pH, acidity and antimicrobial effects of them in dough at ambient temperature over four weeks Checked out.
Based on the results of this study, the minimum inhibitory concentration of these extracts was between 6.25 and 12.5 mg/ml. Among studied extracts, salvia (Salvia officinalis), with a concentration of 0.62 mg/ml significantly decreased the yeast population of Saccharomyces cerevisiae in dough during four weeks (p<0.05). But basil (Ocimum basilicum) extract has only control effect and cinnamon extract has no antimicrobial effect.
Hydroalcoholic extract of salvia (Salvia officinalis), basil (Ocimum basilicum) and cinnamon can be used as an antifungal and antimicrobial agent as a natural preservative in dough and has an important role in consumer health.
S. M. B Hashemi, Sh. Ghorashi, F. Hadizadeh, Z. Zarei, M. Yazdani, M. Noormohammadi,
Volume 19, Issue 7 (12-2017)
Abstract
In this study, the effect of three amplitudes of Ultrasound-Assisted Solvent Extraction (UASE) (0, 20 and 40%; 100W, 30 kHz; the 0% treatment serving as control) on kinetics, thermodynamic, rosmarinic acid content, total phenolics, antioxidant activity, and antimicrobial activity of Ocimum basilicum L. (basil) extract at different temperatures (25, 35 and 45°C) was evaluated. Increases in ultrasound amplitude and temperature increased yields and biological activities of extracts. The highest rosmarinic acid content, total phenolics, antioxidant and antimicrobial activity were obtained for samples treated with UASE at 40% amplitude and 45°C. The kinetics of extraction were evaluated based on a second order mechanism. Increases of amplitude and temperature significantly increased saturated extraction Capacity (Cs), initial extraction rate (h), and rate constant of extraction (k). The thermodynamic aspects of the extraction process showed that samples treated with UASE at 40% amplitude had higher activation Energy (Ea), frequency factor (A), enthalpy (ΔH++) and entropy (ΔS++) than control. UASE at 40% amplitude and control did not significantly differ in thermodynamic parameters. Results also showed very good linear relationships with high correlation coefficients between Ea and ΔH++ and, A and ΔS++. Therefore, ultrasound can affect thermodynamic aspects and kinetics of extraction of basil extract and improve its biological activity.