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Showing 2 results for Agrobacterium Rhizogenes

J. Zolala, M. Farsi, H. R. Gordan, M. Mahmoodnia,
Volume 9, Issue 4 (10-2007)
Abstract

Agrobacterium rhizogenes causes hairy root disease in plants. These hairy roots are ge-netically stable and grow rapidly. Transformed hairy roots of Hyoscyamus muticus in-duced by the bacterium can produce tropane alkaloids in trace amounts of intact plant tissues. In this research, in order to compare growth and biosynthetic stability of hairy roots with wild type ones, leaf and nodal segments of the plant were inoculated with the A. rhizogenes strains A4 and LBA9402. When hairy roots appeared, both the wild type and transformed roots were cultured in a liquid B5 medium. The amounts of tropane alka-loids in both the wild type and transformed roots were measured using HPLC. The growth rates of transformed roots were stable and their dry weights were up to four times higher than those of wild type roots. Alkaloid content was the same for both transformed roots and wild type ones. However, when hairy roots underwent an autonomous dediffer-entiation and produced callus, their hyoscyamine content decreased considerably, whereas not only did their scopolamine content, not decrease, but in some cases it in-creased dramatically. A callus producing hairy root clone, produced 2.72 mg/g scopola-mine which is a significantly high record for hairy roots of H. muticus.
Y. Ahmadi Moghadam, Kh. Piri, B. Bahramnejad, T. Ghiyasvand,
Volume 16, Issue 2 (3-2014)
Abstract

An efficient transformation system for the medicinal plant Portulaca oleracea was established using agropine-type Agrobacterium rhizogenes ATCC15834. Hairy roots were obtained directly from cotyledon leaves explants seven days after inoculation with the bacteria. The highest transformation efficiency was obtained from cotyledon leaves explants, and amounted to 53.3% within two weeks. Roots grew rapidly on solid growth regulator free ½ Murashige and Skoog medium and demonstrated characteristics of transformed roots such as fast growth and high lateral branching. Successful and stable transfer of rolB gene was illustrated by PCR using specific primers of the gene. The hairy roots showed an ability to synthesize natural and medicinal product, dopamine. Elicitation of dopamine production in P. oleracea hairy roots was tested using different concentrations of methyl jasmonate (0, 100, 150, 200 μM) and salicylic acid (0, 125, 250, 500 μM), added to the hairy root cultures during the late growth phase. The results showed that the various concentrations of the methyl jasmonate significantly increased the dopamine content, but, at concentration of 100 μM, its impact was the most pronounced. Salicylic acid had no significant influence on dopamine production in hairy roots of P. oleracea.

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