Volume 16, Issue 1 (2014)                   JAST 2014, 16(1): 203-215 | Back to browse issues page

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Zhang D L, Gao A L, Li Y G, Su Y R, He S B, Li S P. Molecular Characterization and Phylogenetic Analysis of Novel α-gliadin Genes from Triticum dicoccoides L.. JAST. 16 (1) :203-215
URL: http://jast.modares.ac.ir/article-23-1729-en.html
1- College of Life Science, Henan University, Kaifeng, 475001, Henan, People's Republic of China.
Abstract:   (5303 Views)
The quality traits of Triticum dicoccoides KU-13441 (Triticum turgidum L. var. dicoccoides (AABB, 2n= 4x= 28)) were analyzed by mixograph, and the results showed that T. dicoccoides KU-13441 had desirable qualities in gluten strength and flour stirring tolerance. Subsequently, seventeen novel full-ORF α-gliadin genes and thirty-five pseudogenes were cloned and sequenced from T. dicoccoides KU-13441. Among the 17 novel full-ORF α-gliadin genes, the putative proteins of Gli2-TD-44 and Gli2-TD-46 contained an extra cysteine residue, located in the first nonrepetitive region and N-terminal repetitive domain, respectively, rather than in the second nonrepetitive region like other α-gliadins. Prokaryotic expression analysis and western-blotting indicated that these two α-gliadin genes could be successfully expressed under the control of T7 promoter. According to the varying numbers of 4 typical coeliac disease toxic peptides and glutamine residues in the two ployglutamine domains among the 17 α-gliadins, Gli2-TD-39, Gli2-TD-46 and Gli2-TD-47 genes were assigned to sub-genome B and other 14 genes were assigned to sub-genome A. Phylogenetic analysis including two S-genome species, Aegilops longissima (Sl) and Aegilops speltoides (S) revealed that the α-gliadin sequences of the B genome in T. dicoccoides had closer genetic relationship with those from Ae. speltoides. This implies that Ae. speltoides might participate in the origin of wheat B genome.
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Received: 2012/12/28 | Accepted: 2013/07/28 | Published: 2014/01/1

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