Volume 21, Issue 5 (2019)                   JAST 2019, 21(5): 1265-1276 | Back to browse issues page

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1- Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Islamic Republic of Iran.
2- Iranian Biological Resource Center (IBRC), (ACECR), Karaj, Islamic Republic of Iran.
3- Department of Horticulture, Biotechnology and Molecular Genetics, College of Agriculture and Natural Resources, Karaj Islamic Azad University, Alborz, Islamic Republic of Iran.
4- Department of Biology, University of Guilan, Rasht, Islamic Republic of Iran.
Abstract:   (1680 Views)
Artemisia annua L. is an important medicinal plant used as an original source of artemisinin for treating malaria. Although there is a wide distribution of A. annua in Hyrcanian Areas (Mazandaran, Guilan, and Golestan Provinces in N Iran), no considerable effort has been made for diversity assessment. In this study, morphological, phytochemical, and molecular characterization of A. annua accessions in this area were assessed using 4 quantitative characteristics (height, fresh weight, dry weight, and trichome density), artemisinin content, and 15 ISSR primers. Using these traits, a high level of morphological, phytochemical and molecular diversity was revealed among A. annua accessions in the provinces (populations) of Hyrcanian Areas. At inter-population level, the highest value of artemisinin was observed in Mazandaran Province. Moreover, significant correlation between artemisinin content and trichome density was observed that could be useful for indirect selection of artemisinin yield in different accessions of A. annua. In addition, fifteen ISSR primers generated a total number of 222 amplified bands, consisting of 177 and 45 polymorphic and monomorphic loci across the 60 accessions, respectively. These results indicate that ISSR-PCR is a reliable tool for fingerprinting A. annua at the intra-population level. Our results altogether are valid contributions for gene bank management and Artemisia annua breeding programs.
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Article Type: Research Paper | Subject: Agricultural Economics/Agriculture Marketing and Supply Chains
Received: 2016/11/3 | Published: 2019/09/15