Volume 17, Issue 5 (2015)                   JAST 2015, 17(5): 1267-1277 | Back to browse issues page

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1- Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Islamic Republic of Iran.
Abstract:   (6148 Views)
Retrotransposons (RTNs) constitute informative molecular markers for plant species because of their ability to integrate into a multitude of loci throughout the genome and thereby generate insertional polymorphisms between individuals. In the present study, RTN-based molecular markers, IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism), were applied to study RTN integration events and genetic diversity in 100 melon genotypes (88 genotypes from 11 populations, three inbred lines, and 9 hybrids). A total of 94 and 262 loci were amplified using 5 IRAP and 15 REMAP primers, respectively. The percentage of polymorphic loci (PPL) in populations ranged from 39% (Zivari Shahrood) to 48% (Shadegani E). The Mantel test between IRAP and REMAP cophenetic matrices evidenced no significant correlation (r= 0.29). IRAP+REMAP-based cluster analysis using UPGMA algorithm and Dice similarity coefficient depicted 6 groups among 100 melon genotypes. AMOVA revealed the higher level of genetic variation within populations (67%) compared to among populations (33%). The mean Fst values of all groups, except for group VI, were more than 0.20, demonstrating differentiation among the populations and genetic structure of the studied melon collection. 
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Article Type: Research Paper | Subject: Horticultural Science
Received: 2014/05/17 | Accepted: 2014/11/23 | Published: 2015/09/1

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