Tarbiat Modares University Journal of Agricultural Science and Technology 1680-7073 Articles in Press 2025 09 16 Polymorphisms of the self-incompatibility locus of sweet cherry (Prunus avium L.) species from the VNIISPK bioresource collection 24109 EN Aleksandra Guliaeva Russian Research Institute of Fruit Crop Breeding (VNIISPK) Elena Bezlepkina Russian Research Institute of Fruit Crop Breeding (VNIISPK) Journal Article 2025 09 16 Self-incompatibility is a common evolutionary mechanism of flowering plants. Sweet cherry (Prunus avium L.) has a gametophytic type of self-incompatibility. Most of sweet cherry cultivars are self-incompatible. Although self-compatible genotypes also occur. This study presents data of S genotype investigation of 39 sweet cherry cultivars from VNIISPK bioresource collection. To identify S genotypes, PCR with consensus (PaConsI and PaConsII) and allele-specific (S1, S2, S3, S4, S5, S6, S7, S9, S10, S12, S13, S14 and S16) primers was done. The S genotype was fully identified for 33 cultivars. 7 S alleles (S1, S3, S4, S5, S6, S9, and S13) were detected in the genomes of investigated sweet cherries. According to the results of allele-specific amplification, ‘Venera’ (S1x/S4), ‘Zolotuhinskaya’ (S4x/S6) and ‘Viskochka’ (S3/S4x) are suspected to have unique or mutant alleles of the gene. The cultivars with fully determined S-genotypes were distributed into nine incompatibility groups (III, VI, VII, IX, XV, XVII, XIX, XXI, and XLV).

https://jast.modares.ac.ir/article_24109_e1d351219a44905eb5f80749d63ce379.pdf