Tarbiat Modares University Journal of Agricultural Science and Technology 1680-7073 24 6 2022 11 01 Effective Biological Control of Carnation Fusarium Wilt Using a New Combination of Trichoderma Mutant Isolates کنترل زیستی موثر پژمردگی فوزاریوم میخک با استفاده از ترکیب جدید جدایه های جهش یافته تریکودرما 1501 1517 16718 10.52547/jast.24.6.1501 EN F. Zaker Tavallaie Department of Plant Production, Shirvan Agriculture Faculty, University of Bojnord, Bojnord, Islamic Republic of Iran. S. Shahbazi 2 Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Alborz, Islamic Republic of Iran. Z. Daroodi Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Islamic Republic of Iran. Journal Article <span>Fusarium</span><span> infection in carnation is the most important limiting factor for carnation production. We isolated 38 fungal isolates from infected carnation plants collected from Mahallat, Iran, and 15 Fusarium species were identified morphologically. Isolates of </span><span>J14, k5, and k72 </span><span>were the most pathogenic isolates in pathogenicity test on White Liberty cultivar. Molecular identification of </span><span>J14</span><span>, k5, and k72 isolates was done based on Elongation Factor 1-alpha (EF1-α) gene. To investigate the effect of Trichoderma and some of its mutants on reducing the Fusarium infection, six wild type isolates were examined. The species of T. harzianum, T. virense, and T. ghanens showed more inhabitation potential </span><span>and were chosen for induced mutation via gamma irradiation at 250 Gy.</span> <span>The number of </span><span>270 mutants were screened morphologically and 60 mutants were screened using dual culture against J14, k5, and k72. </span><span>Morphological and molecular identification of </span><span>J14, k5, and k72 </span><span>isolates recognized them as </span><span>F. oxysporum. </span><span>Three mutants, i.e. </span><span>ThM7(67.17%), TgM1 (59.45%), and TvM17(57.55%) </span><span>showed the highest efficacy and were selected.</span> <span>Evaluation test of efficacy</span><span> in greenhouse by mixture of T. harzianum, T. virense and T. ghanens (TW) and </span><span>mixture</span><span> of mutant isolates ThM7, TgM1, and TvM17 (TM) showed that biological method had higher ability to control Fusarium infection on </span><span>carnation plants in greenhouse condition, and mutation had no adverse effects</span><span> on plants. The results of this experiment proved that the use of mutation in the Trichoderma genome with the use of gamma radiation could be an effective way to achieve isolates with better performance in this bio-control agent.</span> https://jast.modares.ac.ir/article_16718_ead97089aae476d362a942d978947c32.pdf