Tarbiat Modares University Journal of Agricultural Science and Technology 1680-7073 23 6 2021 11 01 First Nearly Complete Genome Sequence of Onion Yellow Dwarf Virus Infecting Garlic in Iran تعیین اولین ترادف نزدیک به کامل ژنوم ویروس کوتولگی زرد پیاز (Onion yellow dwarf virus) در میزبان سیر از ایران 1355 1366 16607 EN A. Entezari Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Islamic Republic of Iran. M. Mehrvar Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Islamic Republic of Iran. M. Zakiaghl Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Islamic Republic of Iran. Journal Article For the first time, the nearly complete genome sequence of onion yellow dwarf virus (OYDV) was found in Iran from garlic (<em>Allium sativum</em> L.) by deep RNA sequencing. Complete coding sequence of the Iranian isolate of OYDV (MN528769) consists of 10,212 nucleotides (nt), encodes a polyprotein with 3,403 amino acids (aa). Pairwise sequence comparisons showed that IR-Kh2 shares 74.84-97.39% identity at the nt level and 75.67-98% identity at the amino acids level, respectively with other OYDV isolates deposited in the GenBank previously. According to the phylogenetic analysis, the OYDV isolates were divided into two main groups based on the coding sequence of genome and the Iranian OYDV isolate cluster together with the Australian (MS/SW1), Spanish (SG1), Chinese (G78 and G37-2), and Indian (RR1) isolates. Furthermore, a genetic recombination analysis was also performed, in which a putative recombination event was detected in the nuclear inclusion body b (NIb) gene.<span dir="RTL"></span> https://jast.modares.ac.ir/article_16607_b3a5a6df0cf1dfc30882b20f8a493092.pdf