Tarbiat Modares University Journal of Agricultural Science and Technology 1680-7073 21 4 2019 07 01 Genetic Diversity of Stone Fruit Cultivars Preserved On-Farm in Southern Spain تنوع ژنتیکی کولتیوارهای میوه های هسته دار که در مزارع جنوب اسپانیا نگهداری می شود 943 955 16314 EN P. Rallo Department of Agroforestry Sciences, ETSIA, University of Seville, Carretera de Utrera, km 1, 41013 Seville, Spain. M. R. Rocio Jiménez Department of Agroforestry Sciences, ETSIA, University of Seville, Carretera de Utrera, km 1, 41013 Seville, Spain. L. Casanova Department of Agroforestry Sciences, ETSIA, University of Seville, Carretera de Utrera, km 1, 41013 Seville, Spain. A. Morales-Sillero Department of Agroforestry Sciences, ETSIA, University of Seville, Carretera de Utrera, km 1, 41013 Seville, Spain. M. Paz Suárez Department of Agroforestry Sciences, ETSIA, University of Seville, Carretera de Utrera, km 1, 41013 Seville, Spain. Journal Article Old traditional cultivars are valuable genetic resources for crop improvement, but a great number of them have disappeared in the past century. This study aimed to characterize traditional cultivars of different Prunus species collected in small family orchards in southwestern Spain and to evaluate their genetic diversity and relationships. One hundred and twelve accessions belonging to 36 traditional cultivar denominations were analyzed using eight SSR loci transferable across the genus Prunus. The most useful loci to analyze different Prunus species were UDP96-005, BPPCT-002, UDP98-410 and ps02a12. A total of 152 alleles were observed, and 112 were unique to certain species. Sixty-eight different genotypes were found, revealing the possible existence of homonyms among traditional cultivar names. The clustering analysis was consistent with the taxonomic classification of the different species studied and with the geographical origins of the accessions within each species. The results showed wide genetic variability of traditional cultivars of stone fruits grown in small family orchards, which highlights the need to preserve them using both in-situ and ex-situ strategies. Twenty-eight of these accessions are currently conserved ex-situ at the University of Sevilla, Spain. The use of highly transferable SSRs has been proven as efficient in multi-species surveys performed on-farm.

https://jast.modares.ac.ir/article_16314_3b86e315ae7833fe33a2bcdc954aacee.pdf