Tarbiat Modares University Journal of Agricultural Science and Technology 1680-7073 21 1 2019 01 01 Polyphasic Approach Used for Distinguishing Fusarium temperatum from Fusarium subglutinans استفاده ازرویکرد چند فازی برای تشخیصFusarium temperatum از Fusarium subglutinans 221 232 16261 EN J. Levic Maize Research Institute, Zemun Polje, Belgrade-Zemun, Belgrade, Republic of Serbia. F. Munaut Earth and Life Institute, Laboratory of Mycology, Mycology of the Catholic University of Louvain, Catholic University of Louvain, Belgium. J. Scauflaire Earth and Life Institute, Laboratory of Mycology, Mycology of the Catholic University of Louvain, Catholic University of Louvain, Belgium. S. Stankovic Maize Research Institute, Zemun Polje, Belgrade-Zemun, Belgrade, Republic of Serbia. D. Ivanovic Maize Research Institute, Zemun Polje, Belgrade-Zemun, Belgrade, Republic of Serbia. V. Krnjaja Institute for Animal Husbandry, Belgrade-Zemun, Republic of Serbia. Journal Article 1970 01 01 Morphological, biological, and phylogenetic approaches were undertaken for the identification of pathogenic species F. temperatum in the Serbian population of F. subglutinans collected in the 1999-2010 period from Zea mays (3 root, 15 stalk, and 6 seed samples), Sorghum bicolor (two seed samples), Hordeum vulgare (one seed sample) and Taraxacum officinale (one seed sample). Based on interspecies mating compatibility analyses and the maximum parsimony analysis of EF-1α sequences, only two strains, originating from S. bicolor seed (MRIZP 0418 and MRIZP 0552), were identified as F. temperatum, while the remaining 26 single-spore strains were identified as F. subglutinans Group 2. In situ detached barley leaf assay and artificially stalk and ear inoculation of two maize hybrids demonstrated that both F. temperatum and F. subglutinans strains were medium and strong pathogens under laboratory and field conditions, respectively. These are the first data on the F. temperatum as seed-borne pathogens of sorghum, as well as pathogenicity of F. temperatum strains on maize.

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