Tarbiat Modares University Journal of Agricultural Science and Technology 1680-7073 18 3 2016 05 01 Molecular Identification and Characterization of Phosphate Solubilizing Pseudomonas sp. Isolated from Rhizosphere of Mash Bean (Vigna Mungo L.) for Growth Promotion in Wheat 775 788 15893 EN I. Ahmed Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Center, Islamabad-45500, Pakistan. R. Hayat Department of Soil Science and SWC, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan. M. Iqbal Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Center, Islamabad-45500, Pakistan. N. Bibi Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Center, Islamabad-45500, Pakistan. Department of Soil Science and SWC, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan. N. Khalid Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Journal Article Bio-inoculants have potential role in plant growth promotion. The present study evaluated the potential of <em>Pseudomonas</em> strains as bio-inoculants in wheat on the basis of plant growth promotion and physiological characterization. The 16S rRNA gene sequencing and phylogenetic analysis revealed that four isolated strains belonged to genus <em>Pseudomonas</em>. These strains were positive for phosphorus solubilization and indole acetic acid production, whereas only two strains were positive candidate for their nitrogen fixing ability as determined by presence or absence of <em>nifH</em> gene through amplification from polymerase chain reaction. The pot experiment showed that the integrated use of <em>Pseudomonas</em> strains as co-inoculant and 50% applied mineral fertilizers enhanced the maximum wheat growth and development from 58 to 140% for different shoot and root growth parameters. The strain NCCP-45 and NCCP-237 were closely related to<em> Pseudomonas beteli</em> and<em> Pseudomonas lini, </em>respectively. These isolated strains can be used to increase crop productivity by using as a bio-fertilizer inoculum. https://jast.modares.ac.ir/article_15893_e0a30be9b1b30f4d56a2f1e1e760e536.pdf