Tarbiat Modares University Journal of Agricultural Science and Technology 1680-7073 17 6 2015 11 01 In vitro Propagation of Caprifig and Female Fig Varieties (Ficus carica L.) from Shoot-tips 1597 1608 15779 EN Ch. Bayoudh Regional Research Centre for Horticulture and Organic Agriculture (CRRHAB), B. P. 57, 4042 Chott-Mariem, Sousse, Tunisia. R. Labidi Research Unit on Agro-biodiversity, High Agronomic Institute, IRESA-University of Sousse, 4042 Chott-Mariem, Tunisia. A. Majdoub Regional Research Centre for Horticulture and Organic Agriculture (CRRHAB), B. P. 57, 4042 Chott-Mariem, Sousse, Tunisia. M. Mars Regional Research Centre for Horticulture and Organic Agriculture (CRRHAB), B. P. 57, 4042 Chott-Mariem, Sousse, Tunisia. Journal Article Fig trees are threatened by the attack of Fig Mosaic Disease (FMD) on leaves and fruits caused by viruses of several genera. Shoot-tip culture is a convenient method for viral sanitation. For this purpose, a reliable protocol for rapid <em>in vitro</em> propagation was developed with shoot-tips of three major Tunisian local fig (<em>Ficus carica</em> L.) varieties Zidi (ZDI), Soltani (SNI), Bither Abiadh (BA) and one rare and recalcitrant caprifig Assafri (ASF). For each <em>in vitro</em> step, four Murashige and Skoog (MS) media with different combinations of plant regulators were used. The best initiation of shoot-tips with sizes 0.5, 1 and 1.5 mm was obtained on medium M₃ containing 0.2 mg L^-1 Benzyle Amino Purine (BAP), 0.1 mg L^-1 1-NaphthaleneAcetic Acid (NAA) and 0.1 mg L^-1 Gibberellic acid (GA₃). The variety (SNI) showed the highest shoot-tip initiation potentialities for the establishment step with 100% of explant development rate. The shoot multiplication and plantlet development were provided by medium M₆ with 0.5 mg L^-1BAP and 0.1 mg L^-1 NAA. The highest average of leaf number increase (92 leaves per plant) and proliferation rate (16.91 branches per plant) were reached on M₆. The best rooting rate (83.34%) was favored by medium M₁₁ with half-strength MS and 1 mg L^-1 Indole-3-Butyric Acid (IBA). <em>Ex vitro</em> rooting of fig plantlets was successfully performed on moist peat with success rate of 90%. The acclimatized fig vitroplants showed high establishment rates (92.1%) and rapid growth on substrates S₁ composed by peat without symptoms of virus diseases or morphological abnormalities. https://jast.modares.ac.ir/article_15779_080f43a3371b1354a238d626779f4175.pdf